Characterization of an eppin protein complex from human semen and spermatozoa

Biology of Reproduction

Volumn 77, Issue 3, 2007, Pages 476-484

  Wang, Zengjun ^a,b   Widgren, Esther E ^a   Richardson, Richard T ^a   O'Rand, Michael G ^a,c  

^a UNIVERSITY OF NORTH CAROLINA SCHOOL OF MEDICINE   (United States)

^b NANJING MEDICAL UNIVERSITY   (China)

^c UNIVERSITY OF NORTH CAROLINA   (United States)

Author keywords

Clusterin; Contraception; Eppin; Gamete biology; Lactotransferrin; Male reproductive tract; Semenogelin; Seminal plasma; Sertoli cells; Sperm; Spermatozoa; Testis

Indexed keywords

CLUSTERIN; INHIBITOR PROTEIN; LACTOFERRIN; PROTEIN EPPIN; SEMENOGELIN; SEMINAL VESICLE SECRETORY PROTEIN; UNCLASSIFIED DRUG;

ARTICLE; COLUMN CHROMATOGRAPHY; COMPLEX FORMATION; CONTROLLED STUDY; EJACULATION; HUMAN; HUMAN CELL; HUMAN TISSUE; MASS SPECTROMETRY; POLYACRYLAMIDE GEL ELECTROPHORESIS; PRIORITY JOURNAL; PROTEIN ANALYSIS; PROTEIN PROTEIN INTERACTION; SERTOLI CELL; SPERMATOZOON; WESTERN BLOTTING;

BLOTTING, WESTERN; CHROMATOGRAPHY, GEL; CHROMATOGRAPHY, ION EXCHANGE; CLUSTERIN; ELECTROPHORESIS, GEL, TWO-DIMENSIONAL; ELECTROPHORESIS, POLYACRYLAMIDE GEL; HUMANS; IMMUNOHISTOCHEMISTRY; LACTOFERRIN; MALE; PROTEINASE INHIBITORY PROTEINS, SECRETORY; SEMEN; SEMINAL VESICLE SECRETORY PROTEINS; SPECTROMETRY, MASS, MATRIX-ASSISTED LASER DESORPTION-IONIZATION; SPERMATOZOA;

EID: 34548235322     PISSN: 00063363     EISSN: None     Source Type: Journal    
DOI: 10.1095/biolreprod.107.060194     Document Type: Article

References (24)

1. Richardson RT, Sivashanmugam P, Hall SH, Hamil KG, Moore PA, Ruben SM, French FS, O'Rand MG. Cloning and sequencing of human Eppin: a novel family of protease inhibitors expressed in the epididymis and testis. Gene 2001; 270:93-102.
2. Wang Z, Widgren EE, Sivashanmugam P, O'Rand MG, Richardson RT. Association of Eppin with semenogelin on human spermatozoa. Biol Reprod 2005; 72:1064-1070.
3. Sivashanmugam P, Hall SH, Hamil KG, French FS, O'Rand MG, Richardson RT. Characterization of mouse Eppin and a gene cluster of similar protease inhibitors on mouse chromosome 2. Gene 2003; 312:125-134.
4. Robert M, Gagnon C. Semenogelin I: a coagulum forming, multifunctional seminal vesicle protein. Cell Mol Life Sci 1999; 55:944-960.
5. de Lamirande E, Yoshida K, Yoshiike TM, Iwamoto T, Gagnon C. Semenogelin, the main protein of semen coagulum, inhibits human sperm capacitation by interfering with the superoxide anion generated during this process. J Androl 2001; 22:672-679.
6. O'Rand MG, Widgren EE, Wang Z, Richardson RT. Eppin: an effective target for male contraception. Mol Cell Endocrinol 2006; 250:157-162.
7. Robert M, Gibbs BF, Jacobson E, Gagnon C. Characterization of prostate-specific antigen proteolytic activity on its major physiological substrate, the sperm motility inhibitor precursor/semenogelin I. Biochemistry 1997; 36:3811-3819.
8. Yenugu S, Richardson RT, Sivashanmugam P, Wang Z, O'Rand MG, French FS, Hall SH. Antimicrobial activity of human EPPIN, an androgen regulated sperm bound protein with a whey acidic protein motif. Biol Reprod 2004; 71:1484-1490.
9. O'Rand MG, Widgren EE, Sivashanmugam P, Richardson RT, Hall SH, French FS, VandeVoort CA, Ramachandra SG, Ramesh V, Rao AJ. Reversible immunocontraception in male monkeys immunized with Eppin. Science 2004; 306:1189-1190.
10. Wang Z, Widgren EE, Richardson RT, O'Rand MG. Eppin: a molecular strategy for male contraception. In: Roldan E, Gomendio M (eds.), Spermatology. Society for Reproduction and Fertility Volume 65. Nottingham, UK: Nottingham University Press; in press.
11. Shima JE, McLean DJ, McCarrey JR, Griswold MD. The murine testicular transcriptome: characterizing gene expression in the testis during the progression of spermatogenesis. Biol Reprod 2004; 71:319-330.
12. Mammalian Reproductive Genetics. C2007. University of Washington, Seattle, Washington. World Wide Web (http://mrg.genetics.washington.edu/). (January 2007).
13. Sylvester SR, Skinner MK, Griswold MD. A sulfated glycoprotein synthesized by Sertoli cells and by epididymal cells is a component of the sperm membrane. Biol Reprod 1984; 31:1087-1101.
14. Goodman SA, Young LG. Immunological identification of lactoferrin as a shared antigen on radioiodinated human sperm surface and in radioiodinated human seminal plasma. J Reprod Immunol 1981; 3(2):99-108.
15. Jin YZ, Bannai S, Dacheux F, Dacheux JL, Okamura N. Direct evidence for the secretion of lactoferrin and its binding to sperm in the porcine epididymis. Mol Reprod Dev 1997; 47(4):490-496.
16. Andersen OM, Yeung C-H, Vorum H, Wellner M, Andreassen TK, Erdmann B, Mueller EC, Herz J, Otto A, Cooper TG, Willnow TE. Essential role of the apolipoprotein E receptor-2 in sperm development. J Biol Chem 2003; 278:23989-23995.
17. Milo R, Shen-Orr S, Itzkovitz S, Kashtan N, Chklovskii D, Alon U. Network motifs: simple building blocks of complex networks. Science 2002; 298:824-827.
18. Kim PM, Lu LJ, Xia Y, Gerstein MB. Relating three-dimensional structures to protein networks provides evolutionary insights. Science 2006; 314:1938-1941.
19. Bourgeon F, Evrard B, Brillard-Bourdet M, Colleu D, Jegou B, Pineau C. Involvement of semenogelin-derived peptides in the antibacterial activity of human seminal plasma. Biol Reprod 2004; 70:768-774.
20. Matsuda A, Itoh Y, Koshikawa N, Akizawa T, Yana I, Seiki M. Clusterin, an abundant serum factor, is a possible negative regulator of MT6-MMP/MMP-25 produced by neutrophils. J Biol Chem 2003; 278:36350-36357.
21. Robaire B, Viger RS. Regulation of epididymal epithelial cell function. Biol Reprod 1995; 52(2):226-236.
22. Bailey W, Aronow B, Harmony JAK, Griswold MD. Heat shock-initiated apoptosis is accelerated and removal of damaged cells is delayed in the testis of clusterin/ApoJ knock-out mice. Biol Reprod 2002; 66:1042-1053.
23. Jones SE, Jomary C. Clusterin. Int J Biochem Cell Biol 2002; 34:427-431.
24. Suzuki YA, Shin K, Lonnerdal B. Molecular cloning and functional expression of a human intestinal LTF receptor. Biochemistry 2001; 40:15771-15778.