A novel multiplex pcr system for the detection of Staphylococcal Enterotoxin B, TSST, NUC and FEM genes of Staphylococcus Aureus in food system

Journal of Food Safety

Volumn 30, Issue 2, 2010, Pages 443-454

  Shylaja, R ^a   Murali, H S ^a   Batra, H V ^a   Bawa, A S ^a  

^a DEFENCE FOOD RESEARCH LABORATORY   (India)

Author keywords

[No Author keywords available]

Indexed keywords

STAPHYLOCOCCUS; STAPHYLOCOCCUS AUREUS;

EID: 77953097286     PISSN: 01496085     EISSN: 17454565     Source Type: Journal    
DOI: 10.1111/j.1745-4565.2010.00218.x     Document Type: Article

References (23)

1. Balaban N, Rasooly A. Staphylococcal enterotoxins. Int. J. Food. Microbiol. 2000, 61:1-10.
2. Banks MC, Kamel NS, Zabriskie JB, Larone DH, Ursea D, Posnett DN. Staphylococcus aureus express unique superantigens depending on the tissue source. J. Infect. Dis. 2003, 187:77-86.
3. Bergdoll MS. Importance of staphylococci that produce nanogram quantities of enterotoxin. Zentrabl. Bakteriol. 1995, 282:1-6.
4. Brakstad OG, Aasbakk LK, Maeland JA. Detection of Staphylococcus aureus by polymerase chain reaction amplification of the nuc gene. J. Clin. Microbiol. 1992, 30:1654-1660.
5. Breuer K, Wittmann M, Bosche B, Kapp A, Werfel T. Severe atopic dermatitis is associated with sensitization to staphylococcal enterotoxin B (SEB). Allergy 2000, 55:551-555.
6. Francois P, Pittet D, Bento M, Pepey B, Vaudaux P. Rapid detection of methicillin-resistant Staphylococcus aureus directly from sterile or non-sterile clinical samples by a new molecular assay. J. Clin. Microbiol. 2003, 41(1):254-260.
7. Greenfield RA, Brown BR, Hutchins JB, Iandolo JJ, Jackson R, Slater LN, Bronze MS. Microbiological, biological, and chemical weapons of warfare and terrorism. Am. J. Med. Sci. 2002, 323:326-340.
8. Hoofar J, Malorny B, Abdulmawjood A, Cook N, Wagner M, Fach P. Practical considerations in design of internal amplification controls for diagnostic PCR assays. J. Clin. Microbiol. 2004, 42:1863-1868.
9. Jarraud S, Peyrat MA, Lim A, Tristan A, Bes M, Mougel C, Etienne J, Vandenesch F, Bonneville M, Lina G. egc, a highly prevalent operon of enterotoxin gene, forms a putative nursery of superantigens in Staphylococcus aureus. J. Immunol. 2001, 166:669-677.
10. Jarraud S, Mougel C, Thioulouse J, Lina G, Meugnier H, Forey F, Nesme X, Etienne J, Vandenesch F. Relationships between Staphylococcus aureus genetic background, virulence factors, agr groups (alleles), and human disease. Infect. Immun. 2002, 70:631-641.
11. Kumar S, Balakrishna K, Batra HV. Detection of Salmonella enterica serovar Typhi (S. typhi) by selective amplification of invA, viaB, fliC-d and prt genes by polymerase chain reaction in multiplex format. Lett. Appl. Microbiol. 2006, 42:149-154.
12. Mcclure J-A, Conly JM, Lau V, Elsayed S, Louie T, Hutchins W, Zhang K. Novel multiplex PCR assay for detection of the staphylococcal virulence marker Panton-Valentine leukocidin genes and simultaneous discrimination of methicillin-susceptible from - resistant staphylococci. J. Clin. Microbiol. 2006, 44:1141-1144.
13. Mehrotra M, Wang G, Johnson WM. Multiplex PCR for detection of genes for Staphylococcus aureus enterotoxins, exfoliative toxins, toxic shock syndrome toxin 1, and methicillin resistance. J. Clin. Microbiol. 2000, 38:1032-1035.
14. Mempel M, Lina G, Hojka M, Schnopp C, Seidl HP, Schafer T, Ring J, Vandenesch F, Abeck D. High prevalence of superantigens associated with the egc locus in Staphylococcus aureus isolates from patients with atopic eczema. Eur. J. Clin. Microbiol. Infect. Dis. 2003, 22:306-309.
15. Misfeldt ML. Microbial " superantigens." Infect. Immun. 1990, 58:2409-2413.
16. Omoe K, Ishikawa M, Shimoda Y, Hu DL, Ueda S, Shinagawa K. Detection of seg, seh, and sei genes in Staphylococcus aureus isolates and determination of the enterotoxin productivities of S. aureus isolates harboring seg, seh, or sei genes. J. Clin. Microbiol. 2002, 40:857-862.
17. Rajagopalan G, Sen MM, Singh M, Murali NS, Nath KA, Iijima K, Kita H, Leontovich AA, Gopinathan U, Patel R. Intranasal exposure to staphylococcal enterotoxin B elicits an acute systemic inflammatory response. Shock 2006, 25:647-656.
18. Sergeev N, Volokhov D, Chizhikov V, Rasooly A. Simultaneous analysis of multiple staphylococcal enterotoxin genes by an oligonucleotide microarray assay. J. Clin. Microbiol. 2004, 42(5):2134-2143.
19. Sharma NK, Rees CED, Dodd CER. Development of a single-reaction multiplex PCR toxin typing assay for Staphylococcus aureus strains. Appl. Environ. Microbiol. 2000, 66(4):1347-1353.
20. Smyth DS, Hartigan PJ, Meaney WJ, Fitzgerald JR, Deobald CF, Bohach GA, Smyth CJ. Superantigen genes encoded by the egc cluster and SaPIbov are predominant among Staphylococcus aureus isolates from cows, goats, sheep, rabbits and poultry. J. Med. Microbiol. 2005, 54:401-411.
21. Theron J, Morar D, Du Preez M, Brözel VS, Venter SN. A sensitive seminested PCR method for the detection of Shigella in spiked environmental water samples. Water Res. 2001, 35:869-874.
22. Ulrich RG, Sidell S, Taylor TJ, Wilhelmsen CL, Franz DR. Textbook of Military Medicine: Medical Aspects of Chemical and Biological Warfare 2001, 621-630. pp., Office of the Surgeon General, Washington, DC
23. Vannuffel P, Gigi J, Ezzedine H, Vandercam B, Delmee M, Wauters G, Gala J. Specific detection of methicillin-resistant Staphylococcus species by multiplex PCR. J. Clin. Microbiol. 1995, 33:2864-2867.