A sensitive seminested PCR method for the detection of Shigella in spiked environmental water samples

Water Research

Volumn 35, Issue 4, 2001, Pages 869-874

  Theron, J ^a   Morar, D ^a   Du Preez, M ^b   Brozel V S ^a   Venter, S N ^a  

^a UNIVERSITY OF PRETORIA   (South Africa)

^b DSI CSIR Nanotechnology Innovation Centre   (South Africa)

Author keywords

Enrichment; Enteroinvasive E. coli; Ground water; Seminested PCR; Shigella; Surface water

Indexed keywords

CELL CULTURE; ELECTROPHORESIS; ENVIRONMENTAL PROTECTION; ESCHERICHIA COLI;

SEMINESTED POLYMERASE CHAIN REACTION (PCR) METHOD;

BIOLOGICAL WATER TREATMENT;

BACTERIUM LYSATE; DRINKING WATER; GROUND WATER; SURFACE WATER;

ACCURACY; ARTICLE; BACTERIAL GENE; BACTERIAL VIRULENCE; BACTERIUM DETECTION; CONTROLLED STUDY; ESCHERICHIA COLI; NONHUMAN; PLASMID; POLYMERASE CHAIN REACTION; PRIORITY JOURNAL; SEWAGE; SHIGELLA FLEXNERI; WATER CONTAMINATION;

ESCHERICHIA COLI; SHIGELLA; SHIGELLA FLEXNERI;

EID: 0035284518     PISSN: 00431354     EISSN: None     Source Type: Journal    
DOI: 10.1016/S0043-1354(00)00348-1     Document Type: Article

References (20)

1. Polymerase chain reaction-gene probe detection of microorganisms by using filter-concentrated samples
2. Detection of Escherichia coli and Shigella spp. in water using the polymerase chain reaction and gene probes for uid
3. Multi-gene amplification: Simultaneous detection of three virulence genes in diarrhoeal stool
4. Genetic basis of virulence in Shigella species
5. Sequence and molecular characterization of multicopy invasion plasmid antigen gene, ipaH, of Shigella flexneri
6. Use of the polymerase chain reaction and fluorescent-antibody methods for detecting viable and nonculturable Shigella dysenteriae Type 1 in laboratory microcosms
7. Detection of Shigella dysenteriae Type1 and Shigella flexneri in faeces by immunomagnetic isolation and polymerase chain reaction
8. Rapid detection of Shigella dysenteriae and Shigella flexneri in faeces by an immunomagnetic assay with monoclonal antibodies
9. Effectiveness of the Bacteriological Analytical Manual culture method for the recovery of Shigella sonnei from selected foods
10. Detection of Shigella spp. in food with a nested PCR method-sensitivity and performance compared with a conventional culture method
11. Estimating the costs of diarrhoea and epidemic dysentery in KwaZulu-Natal and South Africa
12. Survival of Shigella flexneri on vegetables and detection by polymerase chain reaction
13. Shigellosis from swimming
14. Inhibition of PCR by components of food samples, microbial diagnostic assays and DNA-extraction solutions
15. Facultatively anaerobic gram negative rods. Genus II. Shigella
16. Detection of Shigellae and enteroinvasive Escherichia coli by amplification of the invasion plasmid antigen H DNA sequence in patients with dysentery
17. Sequence variation in two ipaH genes of Shigella flexneri 5 and homology to the LRG-like family of proteins
18. Use of Shigella flexneri ipaC and ipaH gene sequences for the general identification of Shigella spp. and enteroinvasive Escherichia coli
19. Development and testing of invasion-associated DNA probes for detection of Shigella spp. and enteroinvasive Escherichia coli
20. Identification of Shigella species by DNA amplification of different loci of the Shigella virulence plasmid