A novel pathogen detection system based on high-resolution CE-SSCP using a triblock copolymer matrix

Journal of Separation Science

Volumn 33, Issue 11, 2010, Pages 1639-1643

  Shin, Gi Won ^a   Hwang, Hee Sung ^a   Seo, Sang Woo ^a   Oh, Mi Hwa ^b   Ryu, Chang Y ^c   Salvo, Charles J ^c   Feldman, Shaina ^d   Doh, Junsang ^a   Jung, Gyoo Yeol ^a  

^a Pohang University of Science and Technology (POSTECH)   (South Korea)

^b National Institute of Animal Science   (South Korea)

^c Research Rensselaer Polytechnic Institute   (United States)

^d RENSSELAER POLYTECHNIC INSTITUTE   (United States)

Author keywords

CE SSCP; High resolution; Pathogen detection; Poly(ethyleneoxide) poly(propyleneoxide) poly(ethyleneoxide) triblock copolymer

Indexed keywords

BLOCK COPOLYMERS; COPOLYMERIZATION; DNA; GENES; HYDROPHOBICITY; PATHOGENS; RNA;

16S RIBOSOMAL RNA; CONVENTIONAL POLYMERS; DNA FRAGMENT; DYNAMIC COATING; EXPERIMENTAL CONDITIONS; HIGH RESOLUTION; HYDROPHOBIC MOIETIES; HYDROPHOBIC PPO BLOCKS; LOW RESOLUTION; MATRIX; PATHOGEN DETECTION; PEAK BROADENING; POLYMER MATRICES; TRIBLOCK COPOLYMERS;

PLASTIC PRODUCTS;

COPOLYMER; DNA FRAGMENT; ETHYLENE OXIDE DERIVATIVE; PROPYLENE OXIDE; RNA 16S; BACTERIAL DNA; DNA; MICELLE; POLYMER;

ARTICLE; CAPILLARY ELECTROPHORESIS; CONTROLLED STUDY; DNA CONTENT; DNA SEQUENCE; GENE AMPLIFICATION; MICROORGANISM DETECTION; NONHUMAN; PRIORITY JOURNAL; SINGLE STRAND CONFORMATION POLYMORPHISM; VISCOSITY; BACTERIAL INFECTIONS; CHEMISTRY; DEVICES; GENOMICS; ISOLATION AND PURIFICATION; METABOLISM; MICELLE; POLYMERASE CHAIN REACTION; PROCEDURES; STATISTICAL MODEL;

BACTERIAL INFECTIONS; DNA; DNA, BACTERIAL; ELECTROPHORESIS, CAPILLARY; GENOMICS; MICELLES; MODELS, STATISTICAL; POLYMERASE CHAIN REACTION; POLYMERS; POLYMORPHISM, SINGLE-STRANDED CONFORMATIONAL; RNA, RIBOSOMAL, 16S;

EID: 77953051413     PISSN: 16159306     EISSN: 16159314     Source Type: Journal    
DOI: 10.1002/jssc.200900871     Document Type: Article

References (17)

1. Gillman, L. M., Gunton, J., Turenne, C. Y., Wolfe, J., Kabani, A. M., J. Clin. Microbiol. 2001, 39, 3085-3091.
2. Shin, G. W., Cho, Y. S., Hwang, H. S., Park, J. H., Jung, G. Y., Anal. Biochem. 2008, 383, 31-37.
3. Oh, M. H., Paek, S. H., Shin, G. W., Kim, H. Y., Jung, G. Y., Oh, S., J. Food Prot. 2009, 72, 1262-1266.
4. Shin, G. W., Cho, Y. S., Hwang, H. S., Oh, M. H ., Nam, H. G., Park, J. H., Jung, G. Y., Electrophoresis 2009, 30, 2728-2736.
5. Chu, B., Liang, D., J. Chromatogr. A 2002, 966, 1-13.
6. Albarghouthi, M. N., Barron, A. E., Electrophoresis 2000, 21, 4096-4111.
7. Weber, J., Barbier, V., Pages-Berhouet, S., Caux- Moncoutier, V., Stoppa-Lyonnet, D., Viovy, J. L., Anal. Chem. 2004, 76, 4839-4848.
8. Wu, C., Liu, T., Chu, B., Electrophoresis 1998, 19, 231-241.
9. Malmsten, M., Linse, P., Cosgrove, T., Macromolecules 1992, 25, 2474-2481.
10. Rill, R. L., Liu, Y., Ramey, B. A., Van Winkle, D. H., Locke, B. R., Chromatographia 1999, 49, S65-S71.
11. Menchen, S., Johnson, B., Winnik, M. A., Xu, B., Electrophoresis 1996, 17, 1451-1459.
12. Zhang, J., Gassmann, M., He, W., Wan, F., Chu, B., Lab Chip 2006, 6, 526-533.
13. Kourkine, I. V., Hestekin, C. N., Barron, A. E., Electrophoresis 2002, 23, 1375-1385.
14. Ozawa, S., Sugano, K., Sonehara, T., Fukuzono, S., Ichikawa, A., Fukayama, N., Taylor, M., Miyahara, Y., Irie, T., Anal. Chem. 2004, 76, 6122-6129.
15. Byun, I., Park, J., Park, S., Lee, T., Park, T., Biotechnol. Bioprocess Eng. 2008, 13, 96-101.
16. Ellis, L. A., Taylor, C. F., Taylor, G. R., Hum. Mutat. 2000, 15, 556-564.
17. Zhao, C., Xu, G., Gao, P., Yang, J., Shi, X., Tran, J., J. Sep. Sci. 2005, 28, 513-521