Transfusion transmitted Chagas disease: Is it really under control?

Acta Tropica

Volumn 115, Issue 1-2, 2010, Pages 28-34

  Wendel, Silvano ^a  

^a HOSPITAL SÍRIO LIBANÊS   (Brazil)

Author keywords

American trypanosomiasis; Blood transfusion; Chagas disease; Leukofiltration; Pathogen reduction; Protozoa; Transfusional risk

Indexed keywords

ACQUIRED IMMUNE DEFICIENCY SYNDROME; DEVELOPING WORLD; DISEASE CONTROL; DISEASE PREVALENCE; DISEASE TRANSMISSION; HUMAN IMMUNODEFICIENCY VIRUS; PROTOZOAN;

ARTICLE; BLOOD DONOR; BLOOD TRANSFUSION; CHAGAS DISEASE; DEVELOPED COUNTRY; DEVELOPING COUNTRY; HUMAN; INFECTION CONTROL; INFECTION RISK; LYMPHOCYTIC INFILTRATION; MIGRATION; PARASITE SERODIAGNOSIS; PARASITE TRANSMISSION; PATIENT SAFETY; RISK REDUCTION; SCREENING TEST;

BLOOD; BLOOD TRANSFUSION; CHAGAS DISEASE; COMMUNICABLE DISEASE CONTROL; DEVELOPED COUNTRIES; EMIGRATION AND IMMIGRATION; HUMANS; MASS SCREENING; QUESTIONNAIRES; SEROLOGIC TESTS;

PROTOZOA;

EID: 77953026238     PISSN: 0001706X     EISSN: None     Source Type: Journal    
DOI: 10.1016/j.actatropica.2009.12.006     Document Type: Article

References (128)

1. Allain J.P., Bianco C., Blajchman M.A., Brecher M.E., Busch M., Leiby D., Lin L., Stramer S. Protecting the blood supply from emerging pathogens: the role of pathogen inactivation. Transfus. Med. Rev. 2005, 19:110-126.
2. Almeida I.C., Ferguson M.A.J., Schenkman S., Travassos L.R. Lytic anti-alpha-galactosyl antibodies from patients with chronic Chagas disease recognise novel O-linked oligosaccharides on mucin-like GPI-anchored glycoproteins of Trypanosoma cruzi. Biochem. J. 1994, 304:793-802.
3. Almeida I.C., Covas D.T., Soussumi L.M.T., Travassos L.R. A highly sensitive and specific chemiluminescent enzyme-linked immunosorbent assay for diagnosis of active Trypanosoma cruzi infection. Transfusion 1997, 37:850-857.
4. Andrade A.L.S.S., Martelli C.M.T., Luquetti A.O., Oliveira O.S., Silva S.A., Zicker F. Serologic screening for Trypanosoma cruzi among blood donors in central Brazil. Bull. Pan. Am. Health Organ. 1992, 26:157-164.
5. Antaz P.R.Z., Medrano-Mercado N., Torrico F., Ugarte-Fernandez R., Gómez F., Oliveira R.C., Chaves A.C.L., Romanha A.J., Araújo-Jorge T.C. Early, intermediate, and late acute stages in Chagas' disease: a study combining anti-galactose IgG, specific serodiagnosis, and polymerase chain reaction analysis. Am. J. Trop. Med. Hyg. 1999, 61:308-314.
6. Araújo A.B., Vianna E.E., Berne M.E. Anti-Trypanosoma cruzi antibody detection in blood donors in the Southern Brazil. Braz. J. Infect. Dis. 2008, 12(December (6)):480-482.
7. Avila H.A., Sigman D.S., Cohen L.M., Millikan R.C., Simpson L. Polymerase chain reaction of Tripanosoma cruzi kinetoplast minicircle DNA isolated from whole blood lysates: diagnosis of chronic Chagas disease. Mol. Biochem. Parasitol. 1991, 48:211-222.
8. Barea L., Cañavate C., Flores M., Rodriguez M., Bueno J.L., Gozales R., Gárate T., Castro E. Seroprevalence of anti-Trypanosoma cruzi in blood donors coming from Chagasós disease endemic countries. Vox Sang. 2004, 87(Suppl. 3). (abstract W 01.03).
9. Bergoglio R.M. Enfermedad de Chagas post-transfusional. Rev. Med. Cordoba 1965, 53:266-271.
10. Bergoglio R.M. Enfermedad de Chagas post-transfusional. Experiencia clínica de 48 casos. Prensa Med. Arg. 1984, 71:49-52.
11. Berrizbeitia M., Ndao M., Bubis J., Gottschalk M., Aché A., Lacouture S., Medina M., Ward B.J. Field evaluation of four novel enzyme immunoassays for Chagas' disease in Venezuela blood banks: comparison of assays using fixed-epimastigotes, fixed-trypomastigotes or trypomastigote excreted-secreted antigens from two Trypanosoma cruzi strains. Transfus. Med. 2006, 16(December (6)):419-431.
12. Brisse S., Barnabé C., Tibayrenc M. Identification of six Trypanosoma cruzi phylogenetic lineages by random amplified polymorphic DNA and multilocus enzyme. Int. J. Parasitol. 2000, 30:35-44.
13. Britto C., Cardoso M.A., Ravel C., Santoro A., Borges Pereira J., Coura J.R., Morel C.M., Wincker P. Trypanosoma cruzi: parasite detection and strain discrimination in chronic chagasic patients from Northeastern Brazil using PCR amplification of kinetoplast DNA and nonradioactive hybridization. Exp. Parasitol. 1995, 81:462-471.
14. Buekens P., Almendares O., Carlier Y., Dumonteil E., Eberhard M., Gamboa-Leon R., James M., Padilla N., Wesson D., Xiong X. Mother-to-child transmission of Chagas' disease in North America: why don't we do more?. Matern. Child Health J. 2008, 12:283-286.
15. Cardo L.J., Asher L. Transfusion medicine illustrated. Electron micrographic study of the removal of Trypanosoma cruzi from blood products by leukodepletion filters. Transfusion 2006, 46:1067-1068.
16. Cardo L.J., Salata J., Mendez J., Reddy H., Goodrich R. Pathogen inactivation of Trypanosoma cruzi in plasma and platelet concentrates using riboflavin and ultraviolet light. Transfus. Apher. Sci. 2007, 37(October (2)):131-137. (Epub 2007 October 22).
17. Castro E. Photochemical treatment with methylene blue and light as well as freezing of plasma avoids Trypanosoma cruzi transmission by transfusion. Transfusion 2005, 44(Suppl.):SP241.
18. Castro E. Chagas' disease: lessons from routine donation testing. Transfus. Med. 2009, 19(February (1)):16-23.
19. Castro A.M., Luquetti A.O., Rassi A., Rassi G.G., Chiari E., Galvão L.M.C. Blood culture and polymerase chain reaction for the diagnosis of the chronic phase of human infection with Trypanosoma cruzi. Parasitol. Res. 2002, 88:894-900.
20. Castro E., Gironés N., Bueno J.L., Carrión J., Lin L., Fresno M. The efficacy of photochemical treatment with amotosalen HCl and ultraviolet A (INTERCEPT) for inactivation of Trypanosoma cruzi in pooled buffy-coat platelets. Transfusion 2007, 47:434-441.
21. Centers for Disease Control, Prevention (CDC) Chagas disease after organ transplantation - Los Angeles, California, 2006. MMWR Morb. Mortal. Wkly. Rep. 2006, 55:798-800.
22. Cerisola J.A., Rabinovich A., Alvarez M., Di Corleho C.A., Pruneda J. Enfermedad de Chagas y la transfusion de sangre. Bol. Of. Sanit Panam 1972, 63:203-221.
23. Chang C.D., Cheng K.Y., Jiang L.X., Salbilla V.A., Haller A.S., Yem A.W., Bryant J.D., Kirchhoff L.V., Leiby D.A., Schochetman G., Shah D.O. Evaluation of a prototype Trypanosoma cruzi antibody assay with recombinant antigens on a fully automated chemiluminescence analyzer for blood donor screening. Transfusion 2006, 46:1737-1744. (Erratum in: Transfusion. 2006;46:1852).
24. Cheng K.Y., Chang C.D., Salbilla V.A., Kirchhoff L.V., Leiby D.A., Schochetman G., Shah D.O. Immunoblot assay using recombinant antigens as a supplemental test to confirm the presence of antibodies to Trypanosoma cruzi. Clin. Vaccine Immunol. 2007, 14:355-361.
25. Chiari E. Diagnostic tests for Chagas disease. Chagas Disease (American Trypanosomiasis): its Impact on Transfusion and Clinical Medicine 1992, 153-164. ISBT Brazil, São Paulo. S. Wendel, Z. Brener, M.E. Camargo, A. Rassi (Eds.).
26. Chocair P.R., Sabbaga E., Amato Net V., Shiroma M., Goes G.M. Transplante de rim: nova modalidade de transmissão da doença de Chagas. Rev. Med. Trop. Sao Paulo 1981, 23:282-1282.
27. Cimo P.L., Luper W.E., Scouros M.A. Transfusion-associated Chagas' disease in Texas: report of a case. Tex. Med. 1993, 89:48-50.
28. Clark A.K., Kovtunovych G., Kandikar S., Lal S., Stryker G.A. Cloning and expression analysis of two novel paraflagellar rod domain genes found in Trypanosoma cruzi. Parasitol. Res. 2005, 96:312-320.
29. Comeau P. Canadian Blood Services to screen for Chagas disease. CMAJ 2007, 177:242.
30. Corash L. Inactivation of infectious pathogens in labile blood components: meeting the challenge. Transfus. Clin. Biol. 2001, 8:138-145.
31. Costa G.C.V., Teixeira M.G.M., Borges-Pereira J., Castro J.A.F., Coura J.R., Vanderborght B.O.M., Stroops E., Zrein M., Peralta J.M., Oelemann W.M.R. A recombinant antigen and peptide line immunoassay (LIA) as an alternative diagnostic test for Chagas' disease. Mem. Inst. Oswaldo Cruz 1997, 92(Suppl. 1):267.
32. Coura J.R. Chagas disease: what is known and what is needed-a background article. Mem. Inst. Oswaldo Cruz 2007, 102(Suppl. 1):113-122.
33. Coura J.R., Nogueira E.S., Silva J.R. Índices de transmissão da doença de Chagas por transfusão de sangue de doadores na fase crônica da doença. O. Hosp. 1966, 69:115-122.
34. Cura, E., Wendel, S. (Eds.), 1994. Manual de Procedimientos de Control de Calidad para los Laboratorios de Serologia de los Bancos de Sangre.PAHO/HPC/HPT/94.21, Washington, DC, USA.
35. De Paula E.V., Goncales N.S., Xueref S., Addas-Carvalho M., Gilli S.C., Angerami R.N., Goncales F.L. Prevalence of transfusion-transmitted Chagas disease among multitransfused patients in Brazil. BMC Infect. Dis. 2008, 8:5.
36. Dias J.C. Southern Cone Initiative for the elimination of domestic populations of Triatoma infestans and the interruption of transfusional Chagas disease. Historical aspects, present situation, and perspectives. Mem. Inst. Oswaldo Cruz 2007, 102(Suppl. 1):11-18.
37. Dias J.C.P., Silveira A.C., Schofield C.J. The impact of Chagas Disease control in Latin America-a review. Mem. Inst. Oswaldo Cruz 2002, 97:603-612.
38. Docampo R., Moreno S.N.J. The metabolism and mode of action of gentian violet. Drug Metab. Rev. 1990, 22:161-178.
39. Fabron Junior A., Bordin J.O., Moraes-Souza H., FreyMuller E., Lages-Silva E. Removal of Trypanosoma cruzi by white cell-reduction filters: an electronmicroscopic study. Rev. Soc. Bras. Med. Trop. 1999, 32:223-227.
40. Farrugia A. The mantra of blood safety: time for a new tune?. Vox Sang. 2004, 86:1-7.
41. Ferraz A.S., Figueiredo J.F.C. Transmission of Chagas' disease through transplanted kidney: occurrence of the acute form of the disease in two recipients from the same donor. Rev. Inst. Med. Trop. Sao Paulo 1993, 35:461-463.
42. Flores-Chávez M., Fernández B., Puente S., Torres P., Rodríguez M., Monedero C., Cruz I., Gárate T., Cañavate C. Transfusional Chagas disease: parasitological and serological monitoring of an infected recipient and blood donor. Clin. Infect. Dis. 2008, 46(March 1 (5)):e44-e47.
43. Fragata Filho A.A., Correia Ede B., Borges Filho R., Vasconcelos Mde O., Janczuk D., Martins C.S. Sequence of unusual Chagas infection transmissions in the same family: mother by blood transfusion and child congenitally, with a treatment-resistant strain of Trypanosoma cruzi. Rev. Soc. Bras. Med. Trop. 2008, 41:73-75.
44. Freitas J.L.P., Amato V., Sonntag R., Biancalana A., Nussenszweig V., Barreto J.G. Primeiras verificacões de transmissão acidental da molestia de Chagas ao homem por transfusão de sangue. Rev. Paul. Med. 1952, 40:36-40.
45. Furuchó C.R., Umezawa E.S., Almeida I., Freitas V.L., Bezerra R., Nunes E.V., Sanches M.C., Guastini C.M., Teixeira A.R., Shikanai-Yasuda M.A. Inconclusive results in conventional serological screening for Chagas' disease in blood banks: evaluation of cellular and humoral response. Trop. Med. Int. Health 2008, 13:1527-1533.
46. Galvão L.M.C., Chiari E., Macedo A.M., Luquetti A.O., Silva S.A., Andrade A.L.S.S. PCR assay for monitoring Trypanosoma cruzi parasitemia in childhood after specific chemotherapy. J. Clin. Microbiol. 2003, 41:5066-5070.
47. Garraud O., Andreu G., Elghouzzi M.H., Laperche S., Lefrère J.J. Measures to prevent transfusion-associated protozoal infections in non-endemic countries. Travel Med. Infect. Dis. 2007, 5:110-112.
48. Geiseler P.J., Ito J.I., Tegtemeier B.R., Kerndt P.R., Krance R. Fulminant Chagas' disease in bone marrow transplantation. 27th Intersci. Conf. on Antimicrob. Agents and Chemother. (abstract) 1987, 418.
49. Gironés N., Bueno J.L., Carrión J., Fresno M., Castro E. The efficacy of photochemical treatment with methylene blue and light for the reduction of Trypanosoma cruzi in infected plasma. Vox Sang. 2006, 91:285-291.
50. Gomes M.L., Galvão L.M.C., Macedo A.M., Pena S.D.J., Chiari E. Chagas' disease diagnosis: comparative analysis of parasitologic, molecular and serologic methods. Am. J. Trop. Med. Hyg. 1999, 60:205-210.
51. Grant I.H., Gold J.W., Wittner M., Tanowitz H.B., Nathan C., Mayer K., Reich L., Wollner N., Steinherz L., Ghavimi F. Transfusion-associated acute Chagas disease acquired in the United States. Ann. Intern. Med. 1989, 111:849-851.
52. INE, 2009. Instituto Nacional de Estadística. Migraciones iInternacionales por país. Available at (last access November 2009). http://www.ine.es/jaxi/tabla.do%3Fpath=/t42/p02/l0/%26file=02006.px%26type=pcaxis%26L=0.
53. Kerleguer A., Massard S., Janus G., Joussemet M. Chagas disease: screening tests evaluation in a blood military center, prevalence in the French Army. Pathol. Biol. (Paris) 2007, 55:534-538.
54. Kesper N., Almeida K.A., Stolf M.S., Umezawa E.S. Immunoblot analysis of trypomastigote excreted-secreted antigens as a tool for characterization of T. cruzi strains and isolates. J. Parasitol. 2000, 86:862-867.
55. Kirchhoff L.V., Gam A.A., Gusmão R.A., Goldsmith R.S., Rezende J.M., Rassi A. Increased specificity of serodiagnosis of Chagas' disease by detection of antibody to the 72- and 90-kilodalton glycoproteins of Trypanosoma cruzi. J. Infect. Dis. 1987, 155:561-564.
56. Kirchhoff L.V., Paredes P., Lomeli-Guerrero A., Paredes-Espinoza M., Ron-Guerrero C.S., Delgado-Mejia M., Pena-Munoz J.G. Transfusion-associated Chagas disease (American trypanosomiasis) in Mexico: implications for transfusion medicine in the United States. Transfusion 2006, 46:298-304.
57. Kun H., Moore A., Mascola L., Steurer F., Lawrence G., Kubak B., Radhakrishna S., Leiby D., Herron R., Mone T., Hunter R., Kuehnert M. Transmission of Trypanosoma cruzi by Heart Transplantation. Clin. Infect. Dis. 2009, 48(11):1534-1540. Chagas Disease in Transplant Recipients Investigation Team.
58. Lages-Silva E., Crema E., Ramirez L.E., Macedo A.M., Pena S.D., Chiari E. relationship between Trypanosoma cruzi and human chagasic megaesophagus: blood and tissue parasitism. Am. J. Trop. Med. Hyg. 2001, 65:435-441.
59. Leiby D.A., Jensen N.C., Fucci M.C., Stumpf R.J. Prevalence of Trypanosoma cruzi antibodies in a blood donor population at low risk. Transfusion 1997, 37(Suppl. 9S):S297.
60. Leiby D.A., Wendel S., Takaoka D.T., Fachini R.M., Oliveira L.C., Tibbals M.A. Serologic testing for Trypanosoma cruzi: comparison of radioimmunoprecipitation assay with commercially available indirect immunofluorescence assay, indirect hemagglutination assay, and enzyme-linked immunosorbent assay kits. J. Clin. Microbiol. 2000, 38(February (2)):639-642.
61. Leiby D.A., Herron R.M., Garratty G., Herwaldt B.L. Trypanosoma cruzi parasitemia in US blood donors with serologic evidence of infection. J. Infect. Dis. 2008, 198(August 15 (4)):609-613.
62. Lucey C., Weina P.J. Applying the STARD (Standards for Reporting of Diagnostic Accuracy) checklist to the 2007 Transfusion article 'Evaluation of a new enzyme-linked immunosorbent assay for detection of Chagas antibody in US blood donors'. Trans. R. Soc. Trop. Med. Hyg. 2008, 102:155-160.
63. Luquetti A.O. Diagnóstico etiológico da doença de Chagas. Rev. Pat. Trop. 2000, 29(Suppl.):145-149.
64. Marcon G.E.B., Andrade P.D., Albuquerque D.M., Wanderley J.S., Almeida E.A., Guariento M.E., Costa S.C.B. Use of a nested polymerase chain reaction (N-PCR) to detect Trypanosoma cruzi in blood samples from chronic chagasic patients and patients with doubtful serologies. Diagn. Microbiol. Infect. Dis. 2002, 43:39-43.
65. Massad E. The elimination of Chagas' disease from Brazil. Epidemiol. Infect. 2008, 136:1153-1164. (review).
66. Masse M. Universal leukoreduction of cellular and plasma components: process control and performance of the leukoreduction process. Transfus. Clin. Biol. 2001, 8:297-302.
67. Mazza S., Montana A., Benitez C., Juzin E. Transmisión de " Schizotrypanum cruzi" al niño por leche de la madre con enfermedad de Chagas. Publ. MEPRA 1936, 28:41-46.
68. Milei J., Mautner B., Storino R., Sanchez J.A., Ferrans V.S. Does Chagas' disease exist as an undiagnosed form of cardiomyopathy in the United States?. Am. Heart J. 1992, 123:1732-1735. (editorial).
69. Moncayo A. Chagas disease: current epidemiological trends after the interruption of vectorial and transfusional transmission in the southern cone countries. Mem. Inst. Oswaldo Cruz 2003, 98:577-591.
70. Moncayo A., Ortiz Yanine M.I. An update on Chagas disease (human American trypanosomiasis). Ann. Trop. Med. Parasitol. 2006, 100:663-677.
71. Moraes-Souza H., Bordin J.O., Bardossy L., MacPherson D.W., Blajchman M.A. Prevention of transfusion-associated Chagas' disease: efficacy of white cell-reduction filters in removing Trypanosoma cruzi from infected blood. Transfusion 1995, 35:723-736.
72. Muñoz J., Portús M., Corachan M., Fumadó V., Gascon J. Congenital Trypanosoma cruzi infection in a non-endemic area. Trans. R. Soc. Trop. Med. Hyg. 2007, 101:1161-1162.
73. Nickerson P., Orr P., Schoeder M.C., Sekla L., Johnson J. Transfusion associated Trypanosoma cruzi infection in a non endemic area. Ann. Intern. Med. 1989, 111:851-853.
74. Nussenzweig V., Sonntag R., Biancalana A., Freitas J.L.P., Amato Neto V., et al. Ação de corantes tri-fenil-metanicos sobre o Trypanosoma cruzi " in vitro" Emprego da Violeta de Genciana na profilaxia da transmissão da Moléstia de Chagas por transfusão de sangue. O. Hosp. 1953, 44:731-744.
75. O'Brien S.F., Chiavetta J.A., Fan W., Xi G., Yi Q.L., Goldman M., Scalia V., Fearon M.A. Assessment of a travel question to identify donors with risk of Trypanosoma cruzi: operational validity and field testing. Transfusion 2008, 48:755-761.
76. Otani M.M., Vinelli E., Kirchhoff L.V., del Pozo A.E., Sands A., Vercauteren G., Sabino E.C. WHO comparative evaluation of serologic assays for Chagas disease. Transfusion 2009, 49:1076-1082.
77. Panamerican Health Organization - I, 1992. Reunión de la Comisión Intergubernamental del Cono Sur para la Eliminación de T. infestans y la Interrupción de la Transmision de la Tripanosomiasis Americana Transfusional, OPS, OPS/HCP/HCT/PNSP/92.18.
78. Panamerican Health Organization - Iniciativa del Cono Sur, 1997. VI Reunión Intergubernamental para la eliminación de Triatoma infestans y la interrupción de la tripanosomiasis americana por transfusión. Documento OPS/HPC/HCT 98/102, 83 p.
79. Panamerican Health Organization - Va, 2003. Reunión de la Comisión Intergubernamental (CI) de la Iniciativa Andina de Control de la Transmisión Vectorial y Transfusional de Chagas - Guyaquil, Ecuador, Mayo 2003 - OPS. Available at http://www.paho.org/Spanish/AD/DPC/CD/dch-v-inicandina.htm.
80. Panamerican Health Organization (PAHO), 2009. Elegibilidad para la Donación de Sangre. Recomendaciones para la Educación y la Selección de Donantes Potenciales de Sangre. Available at (last accessed November 2009). http://new.paho.org/hq/index.php%3Foption=com_content%26task=view%26id=1449%26Itemid=1270.
81. Pelletier J.P., Transue S., Snyder E.L. Pathogen inactivation techniques. Best Pract. Res. Clin. Haematol. 2006, 19:205-242.
82. Pereira A. Methylene-blue-photoinactivated plasma and its contribution to blood safety. Transfusion 2004, 44:948-950.
83. Piron M., Fisa R., Casamitjana N., López-Chejade P., Puig L., Vergés M., Gascón J., Gómez i Prat J., Portús M., Sauleda S. Development of a real-time PCR assay for Trypanosoma cruzi detection in blood samples. Acta Trop. 2007, 103:195-200.
84. Piron M., Vergés M., Muñoz J., Casamitjana N., Sanz S., Maymó R.M., Hernández J.M., Puig L., Portús M., Gascón J., Sauleda S. Seroprevalence of Trypanosoma cruzi infection in at-risk blood donors in Catalonia (Spain). Transfusion 2008, 48:1862-1868.
85. Prata A. Clinical and epidemiological aspects of Chagas disease. Lancet Infect. Dis. 2001, 1:92-100.
86. Ramirez L.E., Lages-Silva E., Painetti G.M., Rabello R.M.C., Bordin J.O., Souza H.M. Prevention of transfusion-associated Chagas' disease by sterilization of Trypanosoma cruzi-infected blood with gentian violet, ascorbic acid, and light. Transfusion 1995, 35:226-230.
87. Reed S.G., Badaró R., Lloyd R.M.C. Identification of specific and cross-reactive antigens of Leishmania donovani chagasi by human infection sera. J. Immunol. 1987, 138:1598-1601.
88. Remesar M.C., Gamba C., Colaianni I.F., Puppo M., Sartor P.A., Murphy E.L., Neilands T.B., Ridolfi M.A., Leguizamón M.S., Kuperman S., del Pozo A.E. Estimation of sensitivity and specificity of several Trypanosoma cruzi antibody assays in blood donors in Argentina. Transfusion 2009, 49:2352-2358.
89. Rohwedder R.W. Infección chagásica en doadores de sangre y las probabilidades de transmitirla por medio de la transfusion. Bol. Chile Parasitol. 1969, 24:88-93.
90. Sabino E.C., Salles N., Stoops E., Santos M.L., Saez-Alquezar A. Evaluation of Chagas Innolia assay as a confirmatory test for Chagas disease. Mem. Inst. Oswaldo Cruz 1997, 92(Suppl. 1):266.
91. Sabino E.C., Goncalez T.T., Salles N.A., Silva G.R., Chamone D.F. Trends in the prevalence of Chagas' disease among first-time blood donors in Sao Paulo, Brazil. Transfusion 2003, 43:853-856.
92. Salomone O.A., Basquiera A.L., Sembaj A., Aguerri A.M., reyes M.E., Omelianuk M., Fernández R.A., Enders J., Palma A., Barral J.M., Madoery R.J. Trypanosoma cruzi in persons without serologic evidence of disease, Argentina. Emerg. Infect. Dis. 2003, 9:1558-1562.
93. Schlemper B.R. Estudos experimentais de quimioprofilaxia de transmissão da doença de Chagas por transfusão sangüínea. Rev. Patol. Trop. 1978, 7:55-111.
94. Schmunis G.A. Epidemiology of Chagas disease in non-endemic countries: the role of international migration. Mem. Inst. Oswaldo Cruz 2007, 102(Suppl. 1):75-85. (erratum in: Mem Inst Oswaldo Cruz. 2007;102:2p following 1009).
95. Schmunis G.A., Cruz J.R. Safety of the blood supply in Latin America. Clin. Microbiol. Rev. 2005, 18:12-29.
96. Silveira, A.C., 2002. Introdução. In: O controle da doença de Chagas nos países do Cone Sul da América - História de uma Iniciativa Internacional 1991/2001. OPS, pp. 16-41.
97. Silveira-Lacerda E.P., Silva A.G., Junior S.F., Souza M.A., Kesper N., Botelho-Filho A., Umezawa E.S. Chagas' disease: application of TESA-blot in inconclusive sera from a Brazilian blood bank. Vox Sang. 2004, 87:204-207.
98. Souto R.P., Zingales B. Sensitive detection and strain classification of Trypanosoma cruzi by amplification of a ribosomal RNA sequence. Mol. Biochem. Parasitol. 1993, 62:45-52.
99. Souza H.M. The present state of chemoprophylaxis in transfusional Chagas' disease. Rev. Soc. Bras. Med. Trop. 1989, 22:1-3. (editorial).
100. Steele L.S., MacPherson D.W., Kim J., Keystone J.S., Gushulak B.D. The sero-prevalence of antibodies to Trypanosoma cruzi in Latin American refugees and immigrants to Canada. J. Immigr. Minor Health 2007, 9(January (1)):43-47.
101. Tobler L.H., Contestable P., Pitina L., Groth H., Shaffer S., Blackburn G.R., Warren H., Lee S.R., Busch M.P. Evaluation of a new enzyme-linked immunosorbent assay for detection of Chagas antibody in US blood donors. Transfusion 2007, 47(January (1)):90-96. (erratum in: Transfusion. 2007 March;47(3):554. Pitina, Lubor [corrected to Pitina, Lubov]).
102. Umezawa E., Nascimento N.S., Kasper N.J.R., Coura J.R., Borges-Pereira J., Junqueira A.C., Camargo M.E. Immunoblot assay using excreted-secreted antigen of Trypanosoma cruzi in serodiagnosis of congenital, acute, and chronic Chagas disease. J. Clin. Microbiol. 1996, 34:2143-2147.
103. Umezawa E.S., Stolf A.M.S., Corbett C.E.P., Shikanai-Yasuda M.A. Chagas' disease. Lancet 2001, 357:797-799.
104. Van Voorhis W.C., Barrett L.K., Eastman R.T., Alfonso R., Dupuis K. Trypanosoma cruzi inactivation in human platelet concentrates and plasma by a psoralen (amotosalen HCl) and long-wavelength UV. Antimicrob. Agents Chemother. 2003, 47:475-479.
105. Vera-Cruz J.M., Magallón-Gastelum E., Grijalva G., Rincón A.R., Ramos-García C., Armendáriz-Borunda J. Molecular diagnosis of Chagas disease and use of an animal model to study parasite tropism. Parasitol. Res. 2003, 89:480-486.
106. Villalba R., Fornés G., Alvarez M.A., Román J., Rubio V., Fernandez M., Garcia J.M., Viñals M., Torres A. Acute Chagas' disease in a recipient of a bone marrow transplant in Spain: case report. Clin. Infect. Dis. 1992, 14:594-595.
107. Wagner S.J., Skripchenko A., Salata J., Cardo L.J. Photoinactivation of Trypanosoma cruzi in red cell suspensions with thiopyrylium. Transfus. Apher. Sci. 2007, 37(August (1)):23-25. (Epub 2007 August 14).
108. Webert K.E., Cserti C.M., Hannon J., Lin Y., Pavenski K., Pendergrast J.M., Blajchman M.A. Proceedings of a Consensus Conference: pathogen inactivation-making decisions about new technologies. Transfus. Med. Rev. 2008, 22:1-34.
109. Wendel, S., Brener, Z., Camargo, M., Rassi, A. (Eds.), 1992. Chagas disease (American Trypanosomiasis): its impact on transfusion and clinical medicine. ISBT, Brasil'92, SBHH.
110. Wendel S. Doença de Chagas transfusional. Clínica e terapêutica da Doença de Chagas 1997, 411-427. Editora Fiocruz, Rio de Janeiro, Brasil. J.C.P. Dias, J.R. Coura (Eds.).
111. Wendel S. The protozoal parasites - malaria and Chagas' disease. Blood Safety and Surveillance 2003, 355-398. Marcel Dekker, Inc., New York. J.V. Linden, C. Bianco (Eds.).
112. Wendel, S., 2005. Risco residual da transmissão da infecção por Trypanosoma cruzi por via transfusional no Brasil. PhD Thesis. Faculdade de Medicina da Universidade de São Paulo, Brazil, USP/FM/SBD-20/05.
113. Wendel S., Biagini S. Absence of serological surrogate markers for Trypanosoma cruzi infected blood donors. Vox Sang. 1995, 69:44-49.
114. Wendel S., Gonzaga A.L. Chagas disease and blood transfusion: a new world problem?. Vox Sang. 1993, 64:1-12.
115. Wendel S., Leiby D. Parasitic infections in the blood supply. Assessing and countering the threat. Advances in Transfusion Safety Volume IV, Dev Biol (Basel) 2007, vol. 127:17-41. Karger.
116. Wendel S., Takaoka D.T., Jancsó M.M., Mariano M., Leite K., Chioccola V.L.P., Lourenco A.M., Fragata Filho A.A. Is there a role for leukocyte depletion filters to remove Trypanosoma cruzi infection from donor blood?. Bol. Soc. Bras. Hematol. Hemoter. 1995, 17:25-32.
117. Wendel, S., Yasuda, M.A.S., Silva, G.R., 2005. Residual risk for Trypanosoma cruzi transmission by transfusions in Brazil. Transfusion. Suppl Abstract S50-030J.
118. Wendel, S., Yasuda, M.A.S., Silva, G.R., Leiby, D.A., Umezawa, E., Almeida, I., 2005. Confirmatory serological methods for T. cruzi antibodies screening tests: should they be considered as imperfect gold standards? Transfusion. Suppl Abstract S49-030J.
119. WHO-PAHO, 2008. Estandarización y validación del uso clínico de la reacción en cadena de la polimerase para detección de infección por Trypanosoma cruzi. Buenos Aires, 17-22 Noviembre.
120. Winkler M.A., Brashear R.J., Hall H.J., Schur J.D., Pan A.A. Detection of antibodies to Trypanosoma cruzi among blood donors in the southwestern and western United States. II. Evaluation of a supplemental enzyme immunoassay and radioimmuneprecipitation assay for confirmation of seroreactivity. Transfusion 1995, 35:219-225.
121. World Health Assembly, 1998, Resolution WHA.51.14, Geneva.
122. World Health Organization (WHO), 2002. Chagas Disease - TDR Strategic Direction, February. http://www.who.int/tdr/diseases/chagas/direction.htm.
123. World Health Organization (WHO), 2009. Screening Donated Blood for Transfusion-Transmissible Infections. Recommendations. Available at (last access November 2009). http://www.who.int/bloodsafety/ScreeningTTI.pdf.
124. Young C., Losikoff P., Chawla A., Glasser L., Forman E. Transfusion-acquired Trypanosoma cruzi infection. Transfusion 2007, 47(March (3)):540-544.
125. Zhou X.H., Obuchowski N.A., McClish D.K. Statistical Methods in Diagnostic Medicine 2002, John Wiley & Sons, New York.
126. Zicker F., Martelli C.M.T., Andrade A.L.L.S., Silva S.A. Trends of T. cruzi infection based on data from blood bank screening. Rev. Inst. Med. Trop. Sao Paulo 1990, 39:132-137.
127. Zingales B., Souto R.P., Mangia R.H., Lisboa C.V., Campbell D.A., Coura J.R., Jansen A., Fernandes O. Molecular epidemiology of American trypanosomiasis in Brazil based on dimorphisms of rRNA and mini-exon gene sequences. Int. J. Parasitol. 1998, 28:105-112.
128. Zuna H., Recacoechea M., Bermudez H., Romero A., Castedo J. Transmissión de la enfermedad de Chagas por via transfusional en Santa Cruz de la Sierra, Bolivia. Bol. Inf. CENENTROP 1979, 5:49-56.