Use of a nested polymerase chain reaction (N-PCR) to detect Trypanosoma cruzi in blood samples from chronic chagasic patients and patients with doubtful serologies

Diagnostic Microbiology and Infectious Disease

Volumn 43, Issue 1, 2002, Pages 39-43

  Marcon, Gláucia Elisete Barbosa ^a   Andrade, Paula Durante ^a   De Albuquerque, Dulcinéia Martins ^a   Da Wanderley, Jamiro Silva ^a   De Almeida, Eros Antonio ^a   Guariento, Maria Elena ^a   Costa, Sandra Cecília Botelho ^a  

^a UNIVERSITY OF CAMPINAS   (Brazil)

Author keywords

[No Author keywords available]

Indexed keywords

DNA FRAGMENT;

ARTICLE; BLOOD SAMPLING; CHAGAS DISEASE; CHRONIC DISEASE; CLINICAL TRIAL; CONTROLLED CLINICAL TRIAL; CONTROLLED STUDY; DIAGNOSTIC VALUE; DNA DETERMINATION; FEMALE; HUMAN; MAJOR CLINICAL STUDY; MALE; PARASITE IDENTIFICATION; POLYMERASE CHAIN REACTION; PRIORITY JOURNAL; SERODIAGNOSIS; SEROLOGY; STANDARD; TRYPANOSOMA CRUZI; XENODIAGNOSIS;

ANIMALS; BASE SEQUENCE; CHAGAS DISEASE; CHRONIC DISEASE; FEMALE; HUMANS; MALE; POLYMERASE CHAIN REACTION; SENSITIVITY AND SPECIFICITY; SEROLOGIC TESTS; TRYPANOSOMA CRUZI;

EID: 0036277186     PISSN: 07328893     EISSN: None     Source Type: Journal    
DOI: 10.1016/S0732-8893(02)00366-8     Document Type: Article

References (24)

1. Detection of Trypanosoma cruzi in blood specimens of Chronic chagasic Patients by PCR amplification of kinetoplast minicircle DNA: Comparison with serology and xenodiagnosis
2. PCR amplification of T. Cruzi kinetoplast minicircle DNA isolated from whole blood lysates: Diagnosis of chronic Chagas' disease
3. PCR detection: New insights into the diagnosis of Chronic Chagas Disease
4. PCR detection of Trypanosoma cruzi in human blood samples as a tool for diagnosis and treatment evaluation
5. Chagas disease diagnosis using PCR, Hemoculture and Serologic Methods
6. Chagas' Disease diagnosis: Comparative analyses of parasitolologic, molecular and serologic methods
7. Trypanosoma cruzi: Optimization of PCR for detection in human blood
8. Comparison of the PCR with two classic parasitological methods for the diagnosis of Chagas disease in an endemic region of northeastern Brazil
9. PCR detection of Trypanosoma cruzi, African trypanosomes, and Leishmanias species
10. Comparison of PCR and microscopic methods for detecting Trypanosoma cruzi
11. Detection of T. Cruzi by DNA amplification using the PCR
12. Postmortem diagnosis of autochthonous acute chagasic myocarditis by PCR amplification of a species-specific DNA sequence of Trypanosoma cruzi
13. Epidemiology of chagas disease
14. Trypanosoma cruzi e a doenca de chagas
15. An improved, PCR-based strategy for the detection of Trypanosoma cruzi in human blood samples
16. Treatment of congenital Chagas disease diagnosed and followed up by the PCR
17. PCR-based detection of T. Cruzi DNA in serum
18. PCR-based detection in dried feces of Triatoma infestans
19. Enzymatic amplification of β-globin genomic sequences and restriction site analysis for diagnosis of sickle cell anemia
20. Use of the PCR for detecting Trypanosoma cruzi in triatomine vectors
21. Sensitive detection and schizodeme classification of T. Cruzi by amplification of kinetoplast minicircle DNA sequences: Use in diagnosis of Chagas disease
22. Chagas' Disease
23. Use of simplified polymerase chain reaction procedure to detect T. Cruzi in blood samples from chronic chagasic patients in a rural endemic area