MEK1 and MEK2 isoforms regulate distinct functions in pancreatic cancer cells

Oncology Reports

Volumn 24, Issue 1, 2010, Pages 251-255

  Zhou, Lei ^a   Tan, Xiaodong ^a   Kamohara, Hidenobu ^b   Wang, Wei ^a   Wang, Baosheng ^a   Liu, Jingang ^a   Egami, Hiroshi ^c   Baba, Hideo ^b   Dai, Xianwei ^a  

^a CHINA MEDICAL UNIVERSITY   (China)

^b KUMAMOTO UNIVERSITY   (Japan)

^c Ugatake Hospital   (Japan)

Author keywords

Function regulation; MEK2; Mitogen activated protein kinase kinase 1; Pancreatic cancer; RNAi

Indexed keywords

ISOPROTEIN; MESSENGER RNA; MITOGEN ACTIVATED PROTEIN KINASE KINASE 1; MITOGEN ACTIVATED PROTEIN KINASE KINASE 2; SHORT HAIRPIN RNA; ISOENZYME; SMALL INTERFERING RNA;

ANIMAL CELL; ARTICLE; CANCER CELL; CANCER INVASION; CELL CYCLE ARREST; CELL CYCLE G0 PHASE; CELL CYCLE G1 PHASE; CELL FUNCTION; CELL LINE; CELL PROLIFERATION; CELL STRUCTURE; IN VITRO STUDY; MITOSIS; NONHUMAN; NUCLEOTIDE SEQUENCE; PANCREAS CANCER; PRIORITY JOURNAL; ANIMAL; ANTAGONISTS AND INHIBITORS; DRUG EFFECTS; ENZYMOLOGY; GENE EXPRESSION REGULATION; GENE SILENCING; GENETICS; HAMSTER; MESOCRICETUS; METABOLISM; PANCREAS CARCINOMA; PANCREAS TUMOR; PATHOLOGY; PHYSIOLOGY; TUMOR CELL LINE; TUMOR INVASION;

ANIMALS; CARCINOMA, PANCREATIC DUCTAL; CELL LINE, TUMOR; CELL PROLIFERATION; CRICETINAE; GENE EXPRESSION REGULATION, ENZYMOLOGIC; GENE EXPRESSION REGULATION, NEOPLASTIC; GENE KNOCKDOWN TECHNIQUES; ISOENZYMES; MAP KINASE KINASE 1; MAP KINASE KINASE 2; MESOCRICETUS; NEOPLASM INVASIVENESS; PANCREATIC NEOPLASMS; RNA, SMALL INTERFERING;

EID: 77952982440     PISSN: 1021335X     EISSN: 17912431     Source Type: Journal    
DOI: 10.3892/or_00000853     Document Type: Article

References (21)

1. Egami H, Takiyama Y, Cano M, Houser WH and Pour PM: Establishment of hamster pancreatic ductal carcinoma cell line (PC-1) producing blood group-related antigens. Carcinogenesis 10: 861-869, 1989. (Pubitemid 19123432)
2. Egami H, Tomioka T, Tempero M, Kay D and Pour PM: Development of intrapancreatic transplantable model of pancreatic duct adenocarcinoma in Syrian golden hamsters. Am J Pathol 138: 557-561, 1991.
3. Pour PM, Egami H and Takiyama Y: Patterns of growth and metastases of induced pancreatic cancer in relation to the prognosis and its clinical implications. Gastroenterology 100: 529-536, 1991.
4. Ishikawa S, Egami H, Kurizaki T, et al: Analysis of the factor in related to the signal transduction pathway of invasion and metastasis in pancreatic cancer. J Exp Clin Cancer Res 22: 299-306, 2003. (Pubitemid 36734189)
5. Tan X, Egami H, Kamohara H, et al: Involvement of the mitogen-activated protein kinase kinase 2 in the induction of cell dissociation in pancreatic cancer. Int J Oncol 24: 65-73, 2004.
6. Robinson MJ and Cobb MH: Mitogen-activated protein kinase pathway. Curr Opin Cell Biol 9: 180-186, 2003.
7. Cobb MH: MAP kinase pathways. Prog Biophys Mol Biol 71: 479-500, 1999.
8. Kolch W: Meaningful relationships: the regulation of the Ras/Raf/MEK/ERK pathway by protein interactions. Biochem J 351: 289-305, 2000.
9. Bryan AB and Blenis J: Molecular mechanisms mediating mammalian mitogen-activated protein kinase (MAPK) kinase (MEK)-MAPK cell survival signals. Cell Growth Differ 12: 397-408, 2000.
10. Schaeffer HJ and Weber MJ: Mitogen-activated protein kinases: specific messages from ubiquitous messengers. Mol Cell Biol 19: 2435-2444, 1999.
11. Eblen ST, Slack JK, Weber MJ and Catling AD: Rac-PAK signaling stimulates extracellular signal-regulated kinase (ERK) activation by regulating formation of MEK1-ERK complexes. Mol Cell Biol 22: 6023-6033, 2002.
12. Wu X, Noh SJ, Zhou G, Dixon JE and Guan KL: Selective activation of MEK1 but not MEK2 by A-Raf from epidermal growth factor stimulated Hela cells. J Biol Chem 271: 3265-3271, 1996. (Pubitemid 26055630)
13. Xu S, Khoo S, Dang A, et al: Differential regulation of mitogen-activated protein/ERK kinase (MEK)1 and MEK2 and activation by a Ras-independent mechanism. Mol Endocrinol 11: 1618-1625, 1997. (Pubitemid 27435410)
14. Liu L, Zhang Q, Zhang Y, Wang S and Ding Y: Lentivirus-mediated silencing of Tiam I gene influences multiple functions of a human colorectal cancer cell line. Neoplasia 8: 917-924, 2006. (Pubitemid 44706241)
15. Tan X, Egami H, Ishikawa S, et al: Involvement of matrix metalloproteinase-7 in invasion-metastasis through induction of cell dissociation in pancreatic cancer. Int J Oncol 26: 1283-1289, 2005.
16. Giroux S, Tremblay M, Bernard D, et al: Embryonic death of Mek1-deficient mice reveals a role for this kinase in angiogenesis in the labyrinthine region of the placenta. Curr Biol 9: 369-372, 1999. (Pubitemid 29194878)
17. Belanger, LF, Roy S, Tremblay M, et al: Mek2 is dispensable for mouse growth and development. Mol Cell Biol 23: 4778-4787, 2003. (Pubitemid 36793325)
18. Skarpen E, Flinder LI, Rosseland CM, et al: MEK1 and MEK2 regulate distinct functions by sorting ERK2 to different intracellular compartments. FASEB J 22: 466-476, 2008. (Pubitemid 351225583)
19. Tan X, Egami H, Ishikawa S, et al: Relationship between the expression of extracellular signal-regulated kinase 1/2 and the dissociation of pancreatic cancer cells: Involvement of ERK1/2 in the dissociation status of cancer cells. Int J Oncol 24: 815-820, 2004.
20. Favata MF, Hriuchi KY, Manos EJ, et al: Identification of a novel inhibitor of mitogen-activated protein kinase kinase. J Biol Chem 273: 18623-18632, 2002.
21. Silva J, Chang K, Hannon GJ and Rivas FV: RNA-interference-based functional genomics in mammalian cells: reverse genetics coming of age. Oncogene 23: 8401-8409, 2004. (Pubitemid 39540722)