Identification of residues critical for the cell-membrane-penetrating activity of zebrafish neuroglobin

FEBS Letters

Volumn 584, Issue 11, 2010, Pages 2467-2472

  Watanabe, Seiji ^a   Wakasugi, Keisuke ^a,b  

^a UNIVERSITY OF TOKYO   (Japan)

^b JAPAN SCIENCE AND TECHNOLOGY AGENCY   (Japan)

Author keywords

Cell membrane penetrating protein; Fish globin; Neuroglobin; Protein transduction

Indexed keywords

CELL MEMBRANE PROTEIN; LYSINE; NEUROGLOBIN; PROTEIN M1; UNCLASSIFIED DRUG;

AMPHIBIA; ANIMAL CELL; ARTICLE; BIRD; CELL MEMBRANE TRANSPORT; CONTROLLED STUDY; HUMAN; HUMAN CELL; MAMMAL CELL; MOUSE; NONHUMAN; PRIORITY JOURNAL; PROTEIN ANALYSIS; PROTEIN FUNCTION; PROTEIN TRANSPORT; RAT; SITE DIRECTED MUTAGENESIS; SPECIES CONSERVATION; ZEBRA FISH;

ANIMALS; CELL MEMBRANE; CELLS; CELLULAR STRUCTURES; FISHES; GLOBINS; MUTAGENESIS, SITE-DIRECTED; NERVE TISSUE PROTEINS; ZEBRAFISH;

AMPHIBIA; AVES; DANIO RERIO; MAMMALIA; PISCES; VERTEBRATA;

EID: 77952955984     PISSN: 00145793     EISSN: None     Source Type: Journal    
DOI: 10.1016/j.febslet.2010.04.054     Document Type: Article

References (22)

1. Burmester T., Weich B., Reinhardt S., Hankeln T. A vertebrate globin expressed in the brain. Nature 2000, 407:520-523.
2. Sun Y., Jin K., Mao X.O., Zhu Y., Greenberg D.A. Neuroglobin is up-regulated by and protects neurons from hypoxic-ischemic injury. Proc. Natl. Acad. Sci. USA 2001, 98:15306-15311.
3. Sun Y., Jin K., Peel A., Mao X.O., Xie L., Greenberg D.A. Neuroglobin protects the brain from experimental stroke in vivo. Proc. Natl. Acad. Sci. USA 2003, 100:3497-3500.
4. Wakasugi K., Nakano T., Morishima I. Oxidized human neuroglobin as a heterotrimeric Gα protein guanine nucleotide dissociation inhibitor. J. Biol. Chem. 2003, 278:36505-36512.
5. Wakasugi K., Kitatsuji C., Morishima I. Possible neuroprotective mechanism of human neuroglobin. Ann. N.Y. Acad. Sci. 2005, 1053:220-230.
6. Kitatsuji C., Kurogochi M., Nishimura S., Ishimori K., Wakasugi K. Molecular basis of guanine nucleotide dissociation inhibitor activity of human neuroglobin by chemical cross-linking and mass spectrometry. J. Mol. Biol. 2007, 368:150-160.
7. Watanabe S., Wakasugi K. Neuroprotective function of human neuroglobin is correlated with its guanine nucleotide dissociation inhibitor activity. Biochem. Biophys. Res. Commun. 2008, 369:695-700.
8. Awenius C., Hankeln T., Burmester T. Neuroglobins from the zebrafish Danio rerio and the pufferfish Tetraodon nigroviridis. Biochem. Biophys. Res. Commun. 2001, 287:418-421.
9. Fuchs C., Heib V., Kiger L., Haberkamp M., Roesner A., Schmidt M., Hamdane D., Marden M.C., Hankeln T., Burmester T. Zebrafish reveals different and conserved features of vertebrate neuroglobin gene structure, expression pattern, and ligand binding. J. Biol. Chem. 2004, 279:24116-24122.
10. Wakasugi K., Morishima I. Identification of residues in human neuroglobin crucial for guanine nucleotide dissociation inhibitor activity. Biochemistry 2005, 44:2943-2948.
11. Watanabe S., Wakasugi K. Zebrafish neuroglobin is a cell-membrane-penetrating globin. Biochemistry 2008, 47:5266-5270.
12. Go M. Correlation of DNA exonic regions with protein structural units in haemoglobin. Nature 1981, 291:90-92.
13. Schwarze S.R., Ho A., Vocero-Akbani A., Dowdy S.F. In vivo protein transduction: delivery of a biologically active protein into the mouse. Science 1999, 285:1569-1572.
14. Futaki S. Membrane-permeable arginine-rich peptides and the translocation mechanisms. Adv. Drug Deliv. Rev. 2005, 57:547-558.
15. Noguchi H., Bonner-Weir S., Wei F.-Y., Matsushita M., Matsumoto S. BETA2/NeuroD protein can be transduced into cells due to an arginine- and lysine-rich sequence. Diabetes 2005, 54:2859-2866.
16. Chen Y.H., Yang J.T., Martinez H.M. Determination of the secondary structures of proteins by circular dichroism and optical rotatory dispersion. Biochemistry 1972, 11:4120-4131.
17. Mendoza V., Klein D., Ichii H., Ribeiro M.M., Ricordi C., Hankeln T., Burmester T., Pastori R.L. Protection of islets in culture by delivery of oxygen binding neuroglobin via protein transduction. Transplant. Proc. 2005, 37:237-240.
18. Peroni D., Negro A., Bähr M., Dietz G.P.H. Intracellular delivery of neuroglobin using HIV-1 TAT protein transduction domain fails to protect against oxygen and glucose deprivation. Neurosci. Lett. 2007, 421:110-114.
19. Zhou G.Y., Zhou S.N., Lou Z.Y., Zhu C.S., Zheng X.P., Hu X.Q. Translocation and neuroprotective properties of transactivator-of-transcription protein-transduction domain-neuroglobin fusion protein in primary cultured cortical neurons. Biotechnol. Appl. Biochem. 2008, 49:25-33.
20. Dietz G.P.H., Bähr M. Delivery of bioactive molecules into the cell: the Trojan horse approach. Mol. Cell. Neurosci. 2004, 27:85-131.
21. Nakase I., Tadokoro A., Kawabata N., Takeuchi T., Katoh H., Hiramoto K., Negishi M., Nomizu M., Sugiura Y., Futaki S. Interaction of arginine-rich peptides with membrane-associated proteoglycans is crucial for induction of actin organization and macropinocytosis. Biochemistry 2007, 46:492-501.
22. Noguchi H., Ueda M., Matsumoto S., Kobayashi N., Hayashi S. BETA2/NeuroD protein transduction requires cell surface heparan sulfate proteoglycans. Hum. Gene Ther. 2007, 18:10-17.