Short hairpin RNAs specific to human cytomegalovirus terminase subunit pUL89 prevent viral maturation

Antiviral Therapy

Volumn 15, Issue 3, 2010, Pages 391-400

  Thoma, Corina ^a   Bogner, Elke ^a  

^a CHARITÉ UNIVERSITÄTSMEDIZIN BERLIN   (Germany)

Author keywords

[No Author keywords available]

Indexed keywords

HUMAN CYTOMEGALOVIRUS TERMINASE SUBUNIT PUL89; RETROVIRUS VECTOR; SHORT HAIRPIN RNA; UNCLASSIFIED DRUG; VIRUS DNA; VIRUS ENZYME; DEOXYRIBONUCLEASE; SMALL INTERFERING RNA; TERMINASE; UL89 PROTEIN, CYTOMEGALOVIRUS; VIRUS PROTEIN; VIRUS RNA;

ARTICLE; CONTROLLED STUDY; ELECTRON MICROSCOPY; ENZYME SUBUNIT; GENETIC TRANSDUCTION; HUMAN; HUMAN CELL; HUMAN CYTOMEGALOVIRUS; IMMUNOCOMPROMISED PATIENT; NONHUMAN; PRIORITY JOURNAL; PROTEIN EXPRESSION; RNA INTERFERENCE; VIROGENESIS; VIRUS CELL INTERACTION; VIRUS INHIBITION; VIRUS PARTICLE; VIRUS PLAQUE; CELL LINE; CYTOMEGALOVIRUS; DRUG EFFECTS; FIBROBLAST; GENETICS; GROWTH, DEVELOPMENT AND AGING; METABOLISM; VIROLOGY;

CELL LINE; CYTOMEGALOVIRUS; ENDODEOXYRIBONUCLEASES; FIBROBLASTS; HUMANS; RNA INTERFERENCE; RNA, SMALL INTERFERING; RNA, VIRAL; VIRAL PROTEINS;

EID: 77952919201     PISSN: 13596535     EISSN: None     Source Type: Journal    
DOI: 10.3851/IMP1536     Document Type: Article

References (38)

1. Mocarski ES, Liu AC, Spaete RR. Structure and variability of the a sequence in the genome of human cytomegalovirus (Towne strain). J Gen Virol 1987; 68:2223-2230.
2. Tamashiro JC, Spector DH. Terminal structure and heterogeneity in human cytomegalovirus strain AD169. J Virol 1986; 59:591-604.
3. Chou J, Roizman B. The isomerization of the herpes simplex virus 1 genome: identification of the cis-acting and recombination sites within the domain of the a sequence. Cell 1985; 41:803-811. (Pubitemid 15243387)
4. Black LW. DNA packaging in dsDNA bacteriophages. In Calendar R (Editor). The bacteriophages. New York: Plenum Press 1988; pp. 321-373.
5. Casjens S, Huang WM. Initiation of sequential packaging of bacteriophage P22 DNA. J Mol Biol 1982; 157:287-298.
6. Bazinet C, Benbasat J, King J, Carazo JM, Carrascosa JL. Purification and organization of the gene 1 portal protein required for phage P22 DNA packaging. Biochemistry 1988; 27:1849-1856.
7. Guasch A, Pous J, Ibarra B, et al. Detailed architecture of a DNA translocating machine: the high-resolution structure of the bacteriophage Φ29 connector particle. J Mol Biol 2002; 315:663-676. (Pubitemid 34729317)
8. Kochan J, Carrascosa JL, Murialdo H. Bacteriophage lambda preconnectors. Purification and structure. J Mol Biol 1984; 174:433-447. (Pubitemid 15011747)
9. Kocsis E, Cerritelli ME, Trus BL, Cheng N, Steven AC. Improved methods for determination of rotational symmetries in macromolecules. Ultramicroscopy 1995; 60:219-228.
10. Bogner E. Human cytomegalovirus terminase as a target for antiviral chemotherapy. Rev Med Virol 2002; 12:115-127. (Pubitemid 34273046)
11. Bogner E, Radsak K, Stinski MF. The gene product of human cytomegalovirus open reading frame UL56 binds the pac motif and has specific nuclease activity. J Virol 1998; 72:2259-2264. (Pubitemid 28100784)
12. Bogner E, Reschke M, Reis B, Mockenhaupt T, Radsak K. Identification of the gene product encoded by oRF UL56 of the human cytomegalovirus genome. Virology 1993; 196:290-293. (Pubitemid 23247216)
13. Hwang J-S, Bogner E. ATPase activity of the terminase subunit pUL56 of human cytomegalovirus. J Biol Chem 2002; 277:6943-6948. (Pubitemid 34953080)
14. Scheffczik H, Savva CGW, Holzenburg A, Kolesnikova L, Bogner E. The terminase subunits pUL56 and pUL89 of human cytomegalovirus are DNA-metabolizing proteins with toroidal structure. Nucleic Acids Res 2002; 30:1695-1703. (Pubitemid 34679746)
15. Scholz B, Rechter S, Drach JC, Townsend LB, Bogner E. Identification of the ATP-binding site in the terminase subunit pUL56 of human cytomegalovirus. Nucleic Acids Res 2003; 31:1426-1433. (Pubitemid 36304904)
16. Thoma C, Borst E, Messerle M, Rieger M, Hwang J-S, Bogner E. Identification of the interaction domain of the small terminase subunit pUL89 with the large subunit pUL56 of human cytomegalovirus. Biochemistry 2006; 45:8855-8863. (Pubitemid 44100681)
17. Hammond SM, Bernstein E, Beach D, Hannon GJ. An RNA-directed nuclease mediates post-transcriptional gene silencing in Drosophila cells. Nature 2000; 404:293-296. (Pubitemid 30163547)
18. Hutvagner G, McLachlan J, Pasquinelli AE, Balint E, Tuschl T, Zamore PD. A cellular function for the RNA-interference enzyme Dicer in the maturation of the let-7 small temporal RNA. Science 2001; 293:834-838. (Pubitemid 32743968)
19. Giesen K, Radsak K, Bogner E. The potential terminase subunit pUL56 of HCMV is translocated into the nucleus by its own NLS and interacts with importin α. J Gen Virol 2000; 81:2231-2244.
20. Burns JC, Friedmann T, Driever W, Burrascano M, Yee JK. Vesicular stomatitis virus G glycoprotein peudotyped retroviral vectors: concentration to very high titer and efficient gene transfer into mammalian and nonmammalian cells. Proc Natl Acad Sci U S A 1993; 90:8033-8037. (Pubitemid 23260849)
21. Gelderblom HR, Hausmann EHS, Özel MA, Pauli G, Koch MA. Fine structure of human immunodeficiency virus (HIV) and immunolocalization of structural proteins. Virology 1987; 156:171-176.
22. Hwang J-S, Kregler o, Schilf R, et al. Identification of acetylated, tetrahalogenated benzimidazole D-ribonucleotides with enhanced activity against human cytomegalovirus. J Virol 2007; 81:11604-11611.
23. Hannon GJ. RNA interference. Nature 2002; 418:244-251.
24. Tripp RA, Tompkins SM. Therapeutic applications of RNAi for silencing virus replication. Methods Mol Biol 2009; 555:43-61.
25. Stegmeier F, Hu G, Richles RJ, Hannon GJ, Elledge SJ. A lentiviral micoRNA-based system for single-copy polymerase II-regulated RNA interference in mammalian cells. Proc Natl Acad Sci U S A 2005; 102:13212-13217. (Pubitemid 41325003)
26. Sui G, Soohoo C, Affar EB, et al. A DNA vector-based RNAi technology to suppress gene expression in mammalian cells. Proc Natl Acad Sci U S A 2002; 99:5515-5520. (Pubitemid 34411580)
27. Yu J-Y, DeRuiter SL, Turner DL. RNA interference by expression of short-interfering RNAs and hairpin RNAs in mammalian cells. Proc Natl Acad Sci U S A 2002; 99:6047-6052. (Pubitemid 34493262)
28. Brummelkamp TR, Bernards R, Agami R. A system for stable expression of short interfering RNAs in mammalian cells. Science 2002; 296:550-553. (Pubitemid 34408678)
29. Dittmer A, Bogner E. Specific short hairpin RNA-mediated inhibiton of viral DNA packaging of human cytomegalovirus. FEBS Lett 2006; 580:6132-6138. (Pubitemid 44666795)
30. McGeoch DJ, Rixon FJ, Davison AJ. Topics in herpesvirus genomics and evolution. Virus Res 2006; 117:90-104.
31. Baines JD, Poon APW, Rovnak J, Roizmann B. The UL15 gene of herpes simplex virus encodes two proteins and is required for cleavage of viral DNA. J Virol 1994; 68:8118-8124.
32. Sheaffer AK, Newcomb WW, Gao M, et al. Herpes simplex virua DNA cleavage packaging proteins associate with the procapsids prior to its maturation. J Virol 2001; 75:687-698. (Pubitemid 32037272)
33. Wills E, Scholtes L, Baines JD. Herpes simplex virus 1 DNA packaging proteins encoded by UL6, UL15, UL17, UL28 and UL33 are located on the external surface of the viral capsid. J Virol 2006; 80:10894-10899.
34. Haasnoot J, Westerhout EM, Berkhout B. RNA interference against viruses: strike and counterstrike. Nat Biotechnol 2007; 25:1435-1443. (Pubitemid 350233142)
35. Boden D, Push o, Lee F, Tucker L, Ramratnam B. Human immunodeficiency virus type 1 escape from RNA interference. J Virol 2003; 77:11531-11535.
36. Westerhout EM, ooms M, Vink M, Das AT, Berkhout B. HIV-1 can escape from RNA interference by evolving an alternative structure in its RNA genome. Nucleic Acids Res 2005; 33:796-804.
37. Liu YP, Gruber J, Haasnoot J, Konstantinova P, Berkhout B. RNAi-mediated inhibition of HIV-1 by targeting partially complementary viral sequences. Nucleic Acids Res 2009; 37:6194-6204.
38. Champier G, Hantz S, Couvreux A, et al. New functional domains of human cytomegalovirus pUL89 predicted by sequence analysis and three-dimensional modelling of the catalytic site DEXDc. Antivir Ther 2007; 12:217-232. (Pubitemid 46569907)