Lipid bilayer membrane-triggered presynaptic vesicle assembly

ACS Chemical Neuroscience

Volumn 1, Issue 2, 2010, Pages 86-94

  Gopalakrishnan, Gopakumar ^a,b   Thostrup, Peter ^a   Rouiller, Isabelle ^a   Lucido, Anna Lisa ^a,b   Belkaïd, Wiam ^a,b   Colman, David R ^a,b   Lennox, R Bruce ^a  

^a MCGILL UNIVERSITY   (Canada)

^b MCGILL UNIVERSITY   (Canada)

Author keywords

Lipid membranes; Neurodegenerative diseases; Neurons; Regenerative medicine; Supported bilayers; Synapse

Indexed keywords

ANIMAL EXPERIMENT; ANIMAL TISSUE; ARTICLE; BIOCOMPATIBILITY; BLEACHING; CONFOCAL MICROSCOPY; CONTROLLED STUDY; ELECTRICITY; ELECTRON MICROSCOPY; FLUORESCENCE MICROSCOPY; IMMUNOCYTOCHEMISTRY; IN VITRO STUDY; LIPID BILAYER; LIPID MEMBRANE; NERVE CELL NETWORK; NONHUMAN; RAT; SYNAPSE VESICLE; SYNAPTOGENESIS; TRANSMISSION ELECTRON MICROSCOPY;

LIPID MEMBRANES; NEURODEGENERATIVE DISEASES; NEURONS; REGENERATIVE MEDICINE; SUPPORTED BILAYERS; SYNAPSE;

ANIMALS; COCULTURE TECHNIQUES; CRYOELECTRON MICROSCOPY; HIPPOCAMPUS; IMMUNOHISTOCHEMISTRY; LIPID BILAYERS; LIPIDS; MICROSCOPY, CONFOCAL; MICROSCOPY, ELECTRON, TRANSMISSION; NANOPARTICLES; RATS; RECEPTORS, PRESYNAPTIC; SYNAPTIC VESICLES;

EID: 77952918351     PISSN: None     EISSN: 19487193     Source Type: Journal    
DOI: 10.1021/cn900011n     Document Type: Article

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39. In order to further support our suggested mechanism and to exclude other possible mechanisms, we tested whether any membrane fusion related process plays a role. This is because PEG molecules are known to have fusogenic effects when present in either of the membranes that are undergoing membrane fusion (10a, 24). The results imply that SS-BLM/beads made up of the fusogenic DOPC/DOTAP/DPPE-PEG2000 (25:25:50) mixture (10a) were negative for synaptophysin labeling (Figure SI.6, Supporting Information). This suggests that membrane fusion events may be excluded. Though the presence of DOTAP together with DPPE could arguably lead to membrane fusion, lipid exchange, and endocytosis mediated mechanisms (12c), our control experiments suggest that other types of mechanisms are more likely operative. This is further supported by the presence of intact bilayers on the SS-BLMs after 24 h of incubation with hippocampal neurons (Figure SI.7, Supporting Information). Being supported on a solid support could very well reduce the possibility of fusion/endocytosis of the SS-BLM/beads.
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