An improved transconjugation protocol for Bacillus megaterium facilitating a direct genetic knockout

Applied Microbiology and Biotechnology

Volumn 86, Issue 6, 2010, Pages 1959-1965

  Richhardt, Janine ^a   Larsen, Michael ^a   Meinhardt, Friedhelm ^a  

^a UNIVERSITY OF MÜNSTER   (Germany)

Author keywords

Bacillus megaterium; Direct gene knockout; SacB counter selection; Suicide vector; Transconjugation

Indexed keywords

BACILLUS MEGATERIUM; BACILLUS SUBTILIS; DONOR CELLS; E. COLI; GENE KNOCKOUT; GENETIC LOCUS; LEVANSUCRASE; MEGATERIUM; PHYSICAL CONTACTS; SMALL SIZE; SPEED-UPS; SUICIDE VECTORS; TRANSCONJUGANTS; TRANSFER EFFICIENCY; TRANSFERRED DNAS; UV SENSITIVITY; WILD TYPES;

BIODEGRADATION; ESCHERICHIA COLI; VECTORS;

BACTERIOLOGY;

BACTERIUM; BIOTECHNOLOGY; GENE; GENETIC ANALYSIS; MUTATION; SPORULATION;

ARTICLE; BACILLUS MEGATERIUM; BACILLUS SUBTILIS; BACTERIAL STRAIN; BIOTECHNOLOGY; DNA TRANSFER; ESCHERICHIA COLI; GENE LOCUS; KNOCKOUT GENE; NONHUMAN; PASTEURIZATION;

BACILLUS MEGATERIUM; BACILLUS SUBTILIS; CONJUGATION, GENETIC; ESCHERICHIA COLI; GENE KNOCKOUT TECHNIQUES; GENES, BACTERIAL; GENETIC VECTORS; HEXOSYLTRANSFERASES; PLASMIDS;

BACILLUS MEGATERIUM; BACILLUS SUBTILIS; ESCHERICHIA COLI;

EID: 77952876663     PISSN: 01757598     EISSN: None     Source Type: Journal    
DOI: 10.1007/s00253-010-2503-9     Document Type: Article

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