A fluorescence lifetime-based assay for serine and threonine kinases that is suitable for high-throughput screening

Analytical Biochemistry

Volumn 402, Issue 1, 2010, Pages 54-64

  Paterson, Michael J ^a   Dunsmore, Colin J ^b   Hurteaux, Reynald ^c   Maltman, Beatrice A ^b   Cotton, Graham J ^b   Gray, Alexander ^a  

^a UNIVERSITY OF DUNDEE   (United Kingdom)

^b Almac Sciences   (United Kingdom)

^c Edinburgh Instruments   (United Kingdom)

Author keywords

Fluorescence lifetime; High throughput screening; Protein kinase

Indexed keywords

AMINO ACIDS; ENZYMES; IRON COMPOUNDS; PEPTIDES; PHOSPHORYLATION; RADIOMETRY;

FLUORESCENCE INTENSITY MEASUREMENT; FLUORESCENCE LIFETIMES; FLUORESCENT DYES; GOLD STANDARDS; HIGH THROUGHPUT SCREENING; LONG LIFETIME; PROTEIN KINASE; THREONINE KINASE;

FLUORESCENCE;

FERRIC ION; FLUORESCENT DYE; IRON CHELATE; PROTEIN SERINE THREONINE KINASE;

ARTICLE; CONTROLLED STUDY; ENZYME SUBSTRATE; FLUORESCENCE ANALYSIS; FLUORESCENCE LIFETIME BASED ASSAY; HIGH THROUGHPUT SCREENING; PRIORITY JOURNAL; PROTEIN PHOSPHORYLATION; RADIOMETRY;

EID: 77952743004     PISSN: 00032697     EISSN: 10960309     Source Type: Journal    
DOI: 10.1016/j.ab.2010.03.012     Document Type: Article

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