Reduced virus replication, proinflammatory cytokine production, and delayed macrophage cell death in human PBMCs infected with the newly discovered Bundibugyo ebolavirus relative to Zaire ebolavirus

Virology

Volumn 402, Issue 1, 2010, Pages 203-208

  Gupta, Manisha ^a   Goldsmith, Cynthia S ^a   Metcalfe, Maureen G ^a   Spipopoulou, Christina F ^a   Rollin, Pierre E ^a  

^a CENTERS FOR DISEASE CONTROL AND PREVENTION   (United States)

Author keywords

Apoptosis; Cytokines; Ebola virus; Hemorrhagic fever; Macrophages

Indexed keywords

CYTOKINE; INTERLEUKIN 10; INTERLEUKIN 1BETA; MACROPHAGE INFLAMMATORY PROTEIN 1ALPHA; MONOCYTE CHEMOTACTIC PROTEIN 1; TUMOR NECROSIS FACTOR ALPHA;

ARTICLE; BUNDIBUGYO EBOLAVIRUS; CELL DEATH; CYTOKINE PRODUCTION; EBOLA VIRUS; FLOW CYTOMETRY; HEMORRHAGIC FEVER; HUMAN; HUMAN CELL; IN VITRO STUDY; MACROPHAGE; NONHUMAN; PATHOGENESIS; PERIPHERAL BLOOD MONONUCLEAR CELL; PRIORITY JOURNAL; SPECIES; UGANDA; VIRUS REPLICATION; ZAIRE EBOLAVIRUS; CELL CULTURE; COMPARATIVE STUDY; EBOLA HEMORRHAGIC FEVER; EPIDEMIC; IMMUNOLOGY; ISOLATION AND PURIFICATION; PATHOGENICITY; SECRETION; VIROLOGY;

EBOLA VIRUS; ZAIRE EBOLAVIRUS;

CELL DEATH; CELLS, CULTURED; CYTOKINES; DISEASE OUTBREAKS; EBOLAVIRUS; HEMORRHAGIC FEVER, EBOLA; HUMANS; MACROPHAGES; UGANDA; VIRUS REPLICATION;

EID: 77952574977     PISSN: 00426822     EISSN: 10960341     Source Type: Journal    
DOI: 10.1016/j.virol.2010.03.024     Document Type: Article

References (13)

1. Baize S., Leroy E.M., Georges-Courbot M.C., Capron M., Lansoud-Soukate J., Debre P., Fisher-Hoch S.P., McCormick J.B., Georges A.J. Defective humoral responses and extensive intravascular apoptosis are associated with fatal outcome in Ebola virus-infected patients. Nat. Med. 1999, 5(4):423-426.
2. Geisbert T.W., Hensley L.E., Gibb T.R., Steele K.E., Jaax N.K., Jahrling P.B. Apoptosis induced in vitro and in vivo during infection by Ebola and Marburg viruses. Lab. Invest. 2000, 80(2):171-186.
3. Goldsmith C.S., Whistler T., Rollin P.E., Ksiazek T.G., Rota P.A., Bellini W.J., Daszak P., Wong K.T., Shieh W.J., Zaki S.R. Elucidation of Nipah virus morphogenesis and replication using ultrastructural and molecular approaches. Virus Res. 2003, 92(1):89-98.
4. Gupta M., Greer P., Mahanty S., Shieh W.J., Zaki S.R., Ahmed R., Rollin P.E. CD8-mediated protection against Ebola virus infection is perforin dependent. J. Immunol. 2005, 174(7):4198-4202.
5. Gupta M., Mahanty S., Ahmed R., Rollin P.E. Monocyte-derived human macrophages and peripheral blood mononuclear cells infected with ebola virus secrete MIP-1alpha and TNF-alpha and inhibit poly-IC-induced IFN-alpha in vitro. Virology 2001, 284(1):20-25.
6. Gupta M., Spiropoulou C., Rollin P.E. Ebola virus infection of human PBMCs causes massive death of macrophages, CD4 and CD8 T cell sub-populations in vitro. Virology 2007, 364(1):45-54.
7. Hartman A.L., Bird B.H., Towner J.S., Antoniadou Z.A., Zaki S.R., Nichol S.T. Inhibition of IRF-3 activation by VP35 is critical for the high level of virulence of ebola virus. J. Virol. 2008, 82(6):2699-2704.
8. Maynard C.L., Weaver C.T. Diversity in the contribution of interleukin-10 to T-cell-mediated immune regulation. Immunol. Rev. 2008, 226:219-233.
9. Sanchez A., Geisbert T.W., Feldmann H. Fields Virology 2007, 1409-1448. Lippincott Williams and Wilkins, Philadelphia. D.M. Knipe, P.M. Howley (Eds.).
10. Sanchez A., Lukwiya M., Bausch D., Mahanty S., Sanchez A.J., Wagoner K.D., Rollin P.E. Analysis of human peripheral blood samples from fatal and nonfatal cases of Ebola (Sudan) hemorrhagic fever: cellular responses, virus load, and nitric oxide levels. J. Virol. 2004, 78(19):10370-10377.
11. Towner J.S., Sealy T.K., Khristova M.L., Albarino C.G., Conlan S., Reeder S.A., Quan P.L., Lipkin W.I., Downing R., Tappero J.W., Okware S., Lutwama J., Bakamutumaho B., Kayiwa J., Comer J.A., Rollin P.E., Ksiazek T.G., Nichol S.T. Newly discovered ebola virus associated with hemorrhagic fever outbreak in Uganda. PLoS Pathog. 2008, 4(11):e1000212.
12. Villinger F., Rollin P.E., Brar S.S., Chikkala N.F., Winter J., Sundstrom J.B., Zaki S.R., Swanepoel R., Ansari A.A., Peters C.J. Markedly elevated levels of interferon (IFN)-gamma, IFN-alpha, interleukin (IL)-2, IL-10, and tumor necrosis factor-alpha associated with fatal Ebola virus infection. J. Infect. Dis. 1999, 179(Suppl 1):S188-S191.
13. WHO, A. r. f. Ebola Outbreak contained in Uganda. World Health Organization, Features, 22 February, 2008, www.who.int/features/2008/ebola_outbreak/en/.