Fractional polymerization of a suspended planar bilayer creates a fluid, highly stable membrane for ion channel recordings

Journal of the American Chemical Society

Volumn 132, Issue 20, 2010, Pages 7086-7093

  Heitz, Benjamin A ^a   Jones, Ian W ^a   Hall Jr , Henry K ^a   Aspinwall, Craig A ^a   Saavedra, S Scott ^a  

^a UNIVERSITY OF ARIZONA   (United States)

Author keywords

[No Author keywords available]

Indexed keywords

ALAMETHICIN; BINARY COMPOSITION; HIGH-THROUGHPUT; ION CHANNEL; ION-CHANNEL RECORDING; LIPID MEMBRANES; LIPID POLYMERIZATION; LONG TERM STABILITY; MEMBRANE FLUIDITY; PHOSPHATIDYLCHOLINE; PLANAR BILAYERS; PLANAR LIPID MEMBRANES; STABLE MEMBRANES;

BIOASSAY; BIOCHIPS; BIOSENSORS; CHEMICAL STABILITY; FLUIDITY; IONS; MECHANICAL STABILITY; MEMBRANES; PHOSPHOLIPIDS; POLYMERIZATION;

POLYMERS;

ALAMETHICIN; BIS DIENOYL PHOSPHATIDYLCHOLINE; DIPHYTANOYL PHOSPHATIDYLCHOLINE; ION CHANNEL; PHOSPHOLIPID; UNCLASSIFIED DRUG;

ARTICLE; BIOCHIP; BIOSENSOR; LIPID BILAYER; MEMBRANE FLUIDITY; MEMBRANE STABILIZATION; PHOSPHOLIPID MEMBRANE; POLYMERIZATION;

ALAMETHICIN; ION CHANNELS; LIPID BILAYERS; MEMBRANE FLUIDITY; MEMBRANES, ARTIFICIAL; PHOSPHATIDYLCHOLINES; POLYMERS; SUSPENSIONS; TIME FACTORS;

EID: 77952558918     PISSN: 00027863     EISSN: 15205126     Source Type: Journal    
DOI: 10.1021/ja100245d     Document Type: Article

References (62)

1. Tien, H. T. and Ottova, A. L. J. Membr. Sci. 2001, 189, 83-117
2. Tanaka, M. and Sackmann, E. Nature 2005, 437, 656-663
3. Chan, Y. H. M. and Boxer, S. G. Curr. Opin. Chem. Biol. 2007, 11, 581-587
4. Reimhult, E. and Kumar, K. Trends Biotechnol. 2008, 26, 82-89
5. Janshoff, A. and Steinem, C. Anal. Bioanal. Chem. 2006, 385, 433-451
6. Montal, M. and Mueller, P. Proc. Natl. Acad. Sci. U.S.A 1972, 69, 3561-3566
7. Lauger, P. Angew. Chem., Int. Ed. 1985, 24, 905-923
8. Mueller, P., Wescott, W. C., Rudin, D. O., and Tien, H. T. J. Phys. Chem. 1963, 67, 534
9. Tien, H. T. and Diana, A. L. Chem. Phys. Lipids 1968, 2, 55-101
10. Mueller, P., Rudin, D. O., Tien, H. T., and Wescott, W. C. Nature 1962, 194, 979-980
11. Trojanowicz, M. Fresenius J. Anal. Chem 2001, 371, 246-260
12. Zagnoni, M., Sandison, M. E., and Morgan, H. Biosens. Bioelectron. 2009, 24, 1235-1240
13. Ogier, S. D., Bushby, R. J., Cheng, Y. L., Evans, S. D., Evans, S. W., Jenkins, A. T. A., Knowles, P. F., and Miles, R. E. Langmuir 2000, 16, 5696-5701
14. Cheng, Y. L., Bushby, R. J., Evans, S. D., Knowles, P. F., Miles, R. E., and Ogier, S. D. Langmuir 2001, 17, 1240-1242
15. Schmitt, E. K., Vrouenraets, M., and Steinem, C. Biophys. J. 2006, 91, 2163-2171
16. White, R. J., Ervin, E. N., Yang, T., Chen, X., Daniel, S., Cremer, P. S., and White, H. S. J. Am. Chem. Soc. 2007, 129, 11766-11775
17. Maurer, J. A., White, V. E., Dougherty, D. A., and Nadeau, J. L. Biosens. Bioelectron. 2007, 22, 2577-2584
18. Jeon, T. J., Malmstadt, N., and Schmidt, J. J. J. Am. Chem. Soc. 2006, 128, 42-43
19. Malmstadt, N., Jeon, L. J., and Schmidt, J. J. Adv. Mater. 2008, 20, 84-89
20. Jeon, T. J., Malmstadt, N., Poulos, J. L., and Schmidt, J. J. Biointerphases 2008, 3, FA96-FA100
21. Kang, X. F., Cheley, S., Rice-Ficht, A. C., and Bayley, H. J. Am. Chem. Soc. 2007, 129, 4701-4705
22. Ervin, E. N., White, R. J., and White, H. S. Anal. Chem. 2009, 81, 533-537
23. Shenoy, D. K., Barger, W. R., Singh, A., Panchal, R. G., Misakian, M., Stanford, V. M., and Kasianowicz, J. J. Nano Lett. 2005, 5, 1181-1185
24. Daly, S. M., Heffernan, L. A., Barger, W. R., and Shenoy, D. K. Langmuir 2006, 22, 1215-1222
25. Heitz, B. A., Xu, J. H., Hall, H. K., Aspinwall, C. A., and Saavedra, S. S. J. Am. Chem. Soc. 2009, 131, 6662-6663
26. Sisson, T. M., Lamparski, H. G., Kolchens, S., Elayadi, A., and OBrien, D. F. Macromolecules 1996, 29, 8321-8329
27. Boheim, G., Hanke, W., and Eibl, H. Proc. Natl. Acad. Sci. U.S.A 1980, 77, 3403-3407
28. Huang, H. W. and Wu, Y. Biophys. J. 1991, 60, 1079-1087
29. He, K., Ludtke, S. J., Worcester, D. L., and Huang, H. W. Biophys. J. 1996, 70, 2659-2666
30. Andersen, O. S. Annu. Rev. Physiol. 1984, 46, 531-548
31. Wallace, B. A. Annu. Rev. Biophys. Biophys. Chem. 1990, 19, 127-157
32. Ahl, P. L., Price, R., Smuda, J., Gaber, B. P., and Singh, A. Biochim. Biophys. Acta 1990, 1028, 141-153
33. Gaub, H., Buschl, R., Ringsdorf, H., and Sackmann, E. Chem. Phys. Lipids 1985, 37, 19-43
34. Mueller, A. and OBrien, D. F. Chem. Rev. 2002, 102, 727-757
35. Tyminski, P. N., Latimer, L. H., and OBrien, D. F. J. Am. Chem. Soc. 1985, 107, 7769-7770
36. Borle, F., Michel, H., and Sigrist, H. J. Membr. Sci. 1992, 72, 21-30
37. Tyminski, P. N., Latimer, L. H., and OBrien, D. F. Biochemistry 1988, 27, 2696-2705
38. Dorn, K., Klingbiel, R. T., Specht, D. P., Tyminski, P. N., Ringsdorf, H., and OBrien, D. F. J. Am. Chem. Soc. 1984, 106, 1627-1633
39. Benz, R. and Janko, K. Biochim. Biophys. Acta 1976, 455, 721-738
40. Janas, T. Chem. Phys. Lipids 1995, 77, 89-97
41. Sabotin, I., Lebar, A. M., Miklavcic, D., and Kramar, P. IEEE Trans. Dielectr. Electr. Insul. 2009, 16, 1236-1242
42. Lamparski, H. and OBrien, D. F. Macromolecules 1995, 28, 1786-1794
43. Bioelectrochemistry: Fundamentals, Experimental Techniques and Applications, 1 st ed.; Bartlett, P. N., Ed.; John Wiley & Sons Inc.: West Sussex, England, 2008.
44. Ross, E. E., Rozanski, L. J., Spratt, T., Liu, S. C., OBrien, D. F., and Saavedra, S. S. Langmuir 2003, 19, 1752-1765
45. Ross, E. E., Spratt, T., Liu, S. C., Rozanski, L. J., OBrien, D. F., and Saavedra, S. S. Langmuir 2003, 19, 1766-1774
46. Fox, R. O. and Richards, F. M. Nature 1982, 300, 325-330
47. Miick, S. M., Martinez, G. V., Fiori, W. R., Todd, A. P., and Millhauser, G. L. Nature 1992, 359, 653-655
48. Fringeli, U. P. J. Membr. Biol. 1980, 54, 203-212
49. Sansom, M. S. P. Q. Rev. Biophys. 1993, 26, 365-421
50. Huang, H. W. Biochemistry 2000, 39, 8347-8352
51. Cantor, R. S. Biophys. J. 2002, 82, 2520-2525
52. Bechinger, B. J. Membr. Biol. 1997, 156, 197-211
53. Hall, J. E., Vodyanoy, I., Balasubramanian, T. M., and Marshall, G. R. Biophys. J. 1984, 45, 233-247
54. Cafiso, D. S. Annu. Rev. Biophys. Biomol. Struct. 1994, 23, 141-165
55. He, K., Ludtke, S. J., Heller, W. T., and Huang, H. W. Biophys. J. 1996, 71, 2669-2679
56. The requirement for fluidity was verified by preparing BLMs from dipalmitoyl- sn -glycerol-3-phosphocholine (DPPC) which has a main-phase transition temperature of 41 C, well above the measurement temperature of 22 C. No ion channel activity was observed when these gel-phase BLMs were incubated with 60 ng/mL of alamethicin for 15 min (i.e., the data were very similar to that shown in Figure 3 for poly(bis-DenPC)).
57. Subramaniam, V., DAmbruoso, G. D., Hall, H. K., Jr., Wysocki, R. J., Jr., Brown, M. F., and Saavedra, S. S. Langmuir 2008, 24, 11067-11075
58. Ganchev, D. N., Rijkers, D. T. S., Snel, M. M. E., Killian, J. A., and de Kruijff, B. Biochemistry 2004, 43, 14987-14993
59. Constantinescu, I. and Lafleur, M. Biochim. Biophys. Acta Biomembr. 2004, 1667, 26-37
60. Papo, N. and Shai, Y. Biochemistry 2003, 42, 458-466
61. Oliynyk, V., Jager, M., Heimburg, T., Buckin, V., and Kaatze, U. Biophys. Chem. 2008, 134, 168-177
62. Ionov, R., El-Abed, A., Angelova, A., Goldmann, M., and Peretti, P. Biophys. J. 2000, 78, 3026-3035