Purification of porcine reproductive and respiratory syndrome virus from cell culture using ultrafiltration and heparin affinity chromatography

Journal of Chromatography A

Volumn 1217, Issue 21, 2010, Pages 3489-3493

  Hu, Jianzhong ^a   Ni, Yanyan ^b   Dryman, Barbara A ^b   Meng, X J ^b   Zhang, Chenming ^a  

^a Virginia Polytechnic Institute and State University   (United States)

^b CENTER FOR MOLECULAR MEDICINE AND INFECTIOUS DISEASES   (United States)

Author keywords

Heparin affinity chromatography; Membrane ultrafiltration; PRRSV; Virus purification

Indexed keywords

CAUSATIVE AGENTS; DENSITY GRADIENTS; FAST PERFORMANCE LIQUID CHROMATOGRAPHIES; FREEZE/THAW CYCLES; G PROTEIN; HEPARIN AFFINITY; HEPARIN AFFINITY CHROMATOGRAPHY; INFECTIOUS DISEASE; LOW SPEED; MARC-145; MEMBRANE ULTRAFILTRATION; PROCESSING TIME; RETENTATE; ULTRACENTRIFUGATION; VIRAL PARTICLES; VIRUS PURIFICATION;

AFFINITY CHROMATOGRAPHY; CELL CULTURE; CENTRIFUGATION; COLUMN CHROMATOGRAPHY; LIQUID CHROMATOGRAPHY; POLYSACCHARIDES; PROTEINS; ULTRAFILTRATION; VIRUSES;

PURIFICATION;

CELL PROTEIN; HEPARIN; VIRUS PROTEIN;

AFFINITY CHROMATOGRAPHY; ANIMAL CELL; ARTERIVIRUS; ARTICLE; CELL CULTURE; CENTRIFUGATION; CONTROLLED STUDY; CULTURE MEDIUM; DENSITY GRADIENT CENTRIFUGATION; ELUTION; FREEZING; LIQUID CHROMATOGRAPHY; NONHUMAN; POLYACRYLAMIDE GEL ELECTROPHORESIS; PRIORITY JOURNAL; REVERSE TRANSCRIPTION POLYMERASE CHAIN REACTION; SUPERNATANT; THAWING; ULTRAFILTRATION; VIRION; VIRUS PARTICLE; VIRUS PURIFICATION; WESTERN BLOTTING;

ANIMALS; BLOTTING, WESTERN; CELL LINE; CHROMATOGRAPHY, AFFINITY; ELECTROPHORESIS, POLYACRYLAMIDE GEL; FREEZING; HEPARIN; PORCINE REPRODUCTIVE AND RESPIRATORY SYNDROME; PORCINE RESPIRATORY AND REPRODUCTIVE SYNDROME VIRUS; SODIUM CHLORIDE; SWINE; TROMETHAMINE; ULTRAFILTRATION; VIRION; VIRUS CULTIVATION;

PORCINE RESPIRATORY AND REPRODUCTIVE SYNDROME VIRUS; SUIDAE;

EID: 77952548565     PISSN: 00219673     EISSN: None     Source Type: Journal    
DOI: 10.1016/j.chroma.2010.03.023     Document Type: Article

References (37)

1. Zuckerman F.A., Garcia E.A., Luque I.D., Christopher-Hennings J., Doster A., Brito M., Osorio F. Vet. Microbiol. 2007, 123:69.
2. Neumann E.J., Kliebenstein J.B., Johnson C.D., Mabry J.W., Bush E.J., Seitzinger A.H., Green A.L., Zimmerman J.J. J. Am. Vet. Med. Assoc. 2005, 227:385.
3. Dea S., Gagnon C.A., Mardassi H., Rogan D. Arch. Virol. 2000, 145:659.
4. Allende R., Lewis T.L., Lu X., Rock D.L., Kutish G.F., Ali A., Doster A.R., Osorio F.A. J. Gen. Virol. 1999, 80:307.
5. Pol J.M.A., Wagennaar F., Reus J.E.G. Vet. Microbiol. 1997, 55:203.
6. Mardassi H., Massie B., Dea S. Virology 1996, 221:98.
7. Zheng Q., Chen D., Li P., Bi Z., Cao R., Zhou B., Chen P. Virus Gen. 2007, 35:585.
8. Pirzadeh B., Dea S. J. Gen. Virol. 1998, 79:989.
9. Weiland E., Wieczorek-Krohmer M., Kohl D. Vet. Microbiol. 1999, 66:171.
10. Bautista E.M., Molitor T.W. Arch. Virol. 1997, 144:117.
11. Ali A., Roossinck M.J. J. Virol. Methods 2007, 141:84.
12. Rodrigues T., Carrondo M.J.T., Alves P.M., Cruz P.E. J. Biotechnol. 2007, 127:520.
13. Reiser J. Gene Ther. 2000, 7:910.
14. Michel J., Gingery M., Lavelle L. J. Virol. Methods 2004, 122:195-198.
15. Transfiguracion J., Jaalouk D.E., Ghani K., Galipeau J., Kamen A. Hum. Gene Ther. 2003, 14:1139.
16. Loa C.C., Lin T.L., Wu C.C., Bryan T.A., Thacker H.L., Hooper T. J. Virol. Methods 2002, 104:187.
17. Karger A., Bettin B., Granzow H., Mettenleiter T.C. J. Virol. Methods 1998, 70:219.
18. Trilisky E.I., Lenhoff A.M. J. Chromatogr. A 2007, 1142:2.
19. Segura M.M., Kamen A., Trudel P., Garnier A. Biotechnol. Bioeng. 2005, 90:391.
20. Anderson R., Macdonald I., Corbett T., Whiteway A., Prentice H.G. J. Virol. Methods 2000, 85:23.
21. Summerford C., Samulski R.J. Nat. Med. 1999, 5:587.
22. Optiz L., Salaklang J., Buttner H., Reichl U., Wolff M.W. Vaccine 2007, 25:939.
23. Meng X.J., Paul P.S., Halbur P.G. J. Gen. Virol. 1994, 75:1795.
24. Meulenberg J.J.M., Besten A.P., De Kluyver E.P., Moormann R.J.M., Schaaper W.M.M., Wensvoort G. Virology 1995, 206:155.
25. Wu W., Fang Y., Rowland R.R.R., Lawson S.R., Christopher-Hennings J., Yoon K., Nelson E.A. Virus Res. 2005, 114:177.
26. Kramberger P., Peterka M., Boben J., Maja R., Strancar A. J. Chromatogr. A 2007, 1144:143.
27. Lyddiatt A., O' Sullivan D.A. Curr. Opin. Biotechnol. 1998, 9:177.
28. Vanderheijden N., Delputte P.L., Nauwynck H.J., Pensaert M.B. Adv. Exp. Med. Biol. 2001, 494:683.
29. Delputte P.L., Vanderheijden N., Nauwynck H.J., Pensaert M.B. J. Virol. 2002, 76:4312.
30. Delputte P.L., Nauwynck H.J. Adv. Exp. Med. Biol. 2006, 581:247.
31. Matanin B.M., Huang Y.M., Meng X.J., Zhang C. J. Virol. Methods 2008, 147:127.
32. Gonin P., Mardassi H., Gagnon C.A., Massie B., Dea S. Arch. Virol. 1998, 143:1927.
33. Prickett J.R., Kim W., Simer R., Yoon K., Zimmerman J. J. Swine Health Prod. 2008, 16:86.
34. Lyddiatt A. Curr. Opin. Biotechnol. 2002, 13:95.
35. O'Keeffe R.S., Johnston M.D., Slater N.K.H. Biotechnol. Bioeng. 1999, 62:537.
36. Meulenberg J.J.M. Vet. Res. 2007, 3:11.
37. Principles of Virology: Molecular Biology, Pathogenesis and Control: Virus Attachment to Host Cells 2000, 100. ASM Press, Washington, DC. S.J. Flint, L.W. Enquist, R.M. Krug, V.R. Racaniello, A.M. Skalka (Eds.).