Viral receptors and vector purification: New approaches for generating clinical-grade reagents

Nature Medicine

Volumn 5, Issue 5, 1999, Pages 587-558

  Summerford, Candace ^a   Samulski, Richard Jude ^a  

^a UNIVERSITY OF NORTH CAROLINA SCHOOL OF MEDICINE   (United States)

Author keywords

[No Author keywords available]

Indexed keywords

CESIUM CHLORIDE; HEPARIN; IODIXANOL; MONOCLONAL ANTIBODY; MONOCLONAL ANTIBODY A20; REAGENT; UNCLASSIFIED DRUG; VIRUS RECEPTOR;

ADENO ASSOCIATED VIRUS; CENTRIFUGATION; CULTURE MEDIUM; EXPRESSION VECTOR; HIGH PERFORMANCE LIQUID CHROMATOGRAPHY; IMMUNOAFFINITY CHROMATOGRAPHY; PRIORITY JOURNAL; SHORT SURVEY; VIRUS PURIFICATION; VIRUS RECOMBINANT;

EID: 0032917854     PISSN: 10788956     EISSN: None     Source Type: Journal    
DOI: 10.1038/8470     Document Type: Short Survey

References (11)

1. Friedmann, T. in The Development of Human Gene Therapy (Cold Spring Harbor Laboratory Press, Cold Spring Harbor, New York, 1999).
2. Williamsburg BioProcessing Foundation Meeting, Viral Vectors and Vaccines, (The Williamsburg Bioprocessing Foundation, Norfolk, Virginia, 1998.).
3. Summerford, C. & Samulski, R.J. Membrane-associated heparan sulfate proteoglycan is a receptor for adeno-associated virus type 2 vinons. J. Virol. 72, 1438-1445 (1998).
4. Wistuba, A., Kern, A., Weger, S., Grimm, D. & Kleinschmidt, J.A. Subcellular compartmentalization of adeno-associated virus type 2 assembly. J. Virol. 71, 1341-1352 (1997).
5. Grimm, D., Kern, A., Rittner, K. & Kleinschmidt, J.A. Novel tools for production and purification of recombinant adeno-associated virus vectors. Hum. Gene Ther. 9, 2745-2760 (1998).
6. Zolotukhin, S. et al. Recombinant adeno-associated virus purification using novel methods improves infectious titer and yield. Gene Ther. (in the press).
7. Clark, K.R., Liu, X., McGrath, J.P. & Johnson, P.R. Highly purified recombinant adeno-associated virus vectors are biologically active and free of detectable helper and wild type viruses. Hum. Gene Ther. (in the press).
8. Xiao, X., Li, J. & Samulski, R.J. Production of high titer recombinant adeno-associated virus vectors in the absence of helper adenovirus. J. Virol. 72, 2224-2232 (1998).
9. Clark, K.R., Voulgaropoulou, F., Fraley D.M., & Johnson, P.R. Cell lines for the production of infectious recombinant adeno-associated viruses. Hum. Gene Ther. 6, 1329-1341 (1995).
10. Summerford, C., Bartlett, J.S. & Samulski, R.J. αVβ5 integrin: A co-receptor for adeno-associated virus type 2 infection. Nature Med. 5, 78-81 (1999).
11. Qing, K. et al. Human fibroblast growth factor receptor 1 is a co-receptor for infection by adeno-associated virus 2. Nature Med. 5, 71-77 (1999).