A new ELISA plate based microtiter well assay for mycobacterial topoisomerase I for the direct screening of enzyme inhibitory monoclonal antibody supernatants

Journal of Immunological Methods

Volumn 357, Issue 1-2, 2010, Pages 26-32

  Leelaram, Majety Naga ^a   Suneetha, Nunna ^a   Nagaraja, Valakunja ^a,b   Manjunath, Ramanathapuram ^a  

^a INDIAN INSTITUTE OF SCIENCE   (India)

^b JAWAHARLAL NEHRU CENTRE FOR ADVANCED SCIENTIFIC RESEARCH   (India)

Author keywords

Microtiter plate; Monoclonal antibody; Mycobacteria; Rapid screening; Topoisomerase I

Indexed keywords

DNA; DNA TOPOISOMERASE; MONOCLONAL ANTIBODY; NUCLEASE; BACTERIAL PROTEIN; BACTERIUM ANTIBODY;

AGAR GEL ELECTROPHORESIS; ANTIGEN PURIFICATION; ARTICLE; DRUG SCREENING; ENZYME ACTIVITY; ENZYME LINKED IMMUNOSORBENT ASSAY; ENZYME SUBSTRATE; HYBRIDOMA; MICROTITER PLATE ASSAY; NONHUMAN; PRIORITY JOURNAL; SUPERNATANT; TISSUE CULTURE; VALIDITY; ANIMAL; BAGG ALBINO MOUSE; CHEMISTRY; DRUG ANTAGONISM; ENZYMOLOGY; FEMALE; IMMUNOLOGY; METHODOLOGY; MOUSE; MYCOBACTERIUM;

CORYNEBACTERINEAE;

ANIMALS; ANTIBODIES, BACTERIAL; ANTIBODIES, MONOCLONAL; BACTERIAL PROTEINS; DNA TOPOISOMERASES, TYPE I, BACTERIAL; ENZYME-LINKED IMMUNOSORBENT ASSAY; FEMALE; MICE; MICE, INBRED BALB C; MYCOBACTERIUM;

EID: 77952474626     PISSN: 00221759     EISSN: None     Source Type: Journal    
DOI: 10.1016/j.jim.2010.03.008     Document Type: Article

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