Identification of crystallin modifications in the human lens cortex and nucleus using laser capture microdissection and CyDye labeling

Molecular Vision

Volumn 16, Issue , 2010, Pages 476-494

  Asomugha, C O ^a   Gupta, R ^a   Srivastava, O P ^a  

^a UNIVERSITY OF ALABAMA AT BIRMINGHAM   (United States)

Author keywords

[No Author keywords available]

Indexed keywords

ALPHA CRYSTALLIN; BETA CRYSTALLIN; CRYSTALLIN; FILENSIN; GAMMA CRYSTALLIN; INDOCYANINE GREEN; INTERMEDIATE FILAMENT PROTEIN; PHAKININ; UNCLASSIFIED DRUG; FLUORESCENT DYE;

ACETYLATION; AGED; AGING; ARTICLE; CARBAMOYLATION; CELL MIGRATION; DEAMINATION; ETHYLATION; HUMAN; HUMAN TISSUE; LASER CAPTURE MICRODISSECTION; LENS; LENS NUCLEUS; MASS SPECTROMETRY; METHYLATION; MOLECULAR WEIGHT; OXIDATION; PHOSPHORYLATION; PRIORITY JOURNAL; PROTEIN AGGREGATION; PROTEIN ANALYSIS; PROTEIN ISOLATION; PROTEIN LOCALIZATION; PROTEIN MODIFICATION; PROTEIN PROCESSING; STAINING; TWO DIMENSIONAL GEL ELECTROPHORESIS; CELL NUCLEUS; CHEMISTRY; CYTOLOGY; ELECTROSPRAY MASS SPECTROMETRY; FLUORESCENCE; ISOLATION AND PURIFICATION; LASER; METABOLISM; METHODOLOGY; MICRODISSECTION;

AGED; CELL NUCLEUS; CRYSTALLINS; ELECTROPHORESIS, GEL, TWO-DIMENSIONAL; FLUORESCENCE; FLUORESCENT DYES; HUMANS; LASERS; LENS, CRYSTALLINE; MICRODISSECTION; PROTEIN PROCESSING, POST-TRANSLATIONAL; SPECTROMETRY, MASS, ELECTROSPRAY IONIZATION; SPECTROMETRY, MASS, MATRIX-ASSISTED LASER DESORPTION-IONIZATION; STAINING AND LABELING;

EID: 77952301017     PISSN: None     EISSN: 10900535     Source Type: Journal    
DOI: None     Document Type: Article

References (60)

1. Delaye M, Tardieu A. Short-range order of crystallin proteins accounts for eye lens transparency. Nature 1983; 302:415-417. [PMID: 6835373]
2. Ponce A, Sorensen C, Takemoto L. Role of short-range protein interactions in lens opacifications. Mol Vis 2006; 12:879-884. [PMID: 16917488]
3. Andley UP. Crystallins in the eye: function and pathology. Prog Retin Eye Res 2007; 26:78-98. [PMID: 17166758]
4. Bloemendal H, de Jong W, Jaenicke R, Lubsen NH, Slingsby C, Tardieu A. Ageing and vision: structure, stability and function of lens crystallins. Prog Biophys Mol Biol 2004; 86:407-485. [PMID: 15302206]
5. Harding JJ. Lens. In: Harding, JJ, editor. Biochemistry of the Eye. London: Chapman & Hall; 1997. p. 94-134.
6. Horwitz J. α-Crystallin can function as a molecular chaperone. Proc Natl Acad Sci USA 1992; 89: 10449-10453. [PMID: 1438232]
7. Derham BK, Harding JJ. Effect of aging on the chaperone-likefunction of human α-crystallin assessed by three methods. Biochem J 1997; 328:763-768. [PMID: 9396718]
8. Muchowski PJ, Bassuk JA, Lubsen NH, Clark JI. Human αBcrystallin. J Biol Chem 1997; 272:2578-2582. [PMID: 8999975]
9. Cohen D, Bar-Yosef U, Levy J, Gradstein L, Belfair N, Ofir R, Joshua S, Lifshitz T, Carmi R, Birk OS. Homozygous CRYBB1 deletion mutation underlies autosomal recessive congenital cataract. Invest Ophthalmol Vis Sci 2007; 48:2208-2213. [PMID: 17460281]
10. Ferrini W, Schorderet DF, Othenin-Girard P, Uffer S, Heon E, Munier FL. CRYBA3/A1 gene mutation associated withsuture-sparing autosomal dominant congenital nuclear cataract: a novel phenotype. Invest Ophthalmol Vis Sci 2004; 45:1436-1441. [PMID: 15111599]
11. Lu S, Zhao C, Jiao H, Kere J, Tang X, Zhao F, Zhang X, Zhao K, Larsson C. Two Chinese families with pulverulent congenital cataracts and deltaG91 CRYBA1 mutations. Mol Vis 2007; 13:1154-1160. [PMID: 17653060]
12. Takemoto L, Boyle D. Determination of the in vivo deamidationrate of asparagines-101 from alpha-A crystallin using microdissected sections of the aging human lens. Exp Eye Res 1998; 67:119-120. [PMID: 9702185]
13. Takemoto LJ. Quantitation of asparagines-101 deamidationfrom alpha-A crystallin during again of the human lens. Curr Eye Res 1998; 17:247-250. [PMID: 9543632]
14. Andley UP, Clark JA. Generation of oxidants in the near UV photooxidation of human lens alpha-crystallin. Invest Ophthalmol Vis Sci 1989; 30:706-713. [PMID: 2703311]
15. McDermott M, Chiesa R, Roberts JE, Dillon J. Photooxidation of specific residues of α-crystallin. Biochemistry 1991; 30:8653-8660. [PMID: 1888728]
16. Spector A. The search for a solution to senile cataracts. Procter lecture. Invest Ophthalmol Vis Sci 1984; 25:130-145. [PMID:6321383]
17. Nagaraj RH, Sell DR, Prabhakaram M, Ortwerth BJ, Monnier VM. High correlation between pentosidine protein crosslinksand pigmentation implicates ascorbate oxidation in human lens senescence and cataractogenesis. Proc Natl Acad Sci USA 1991; 88:10257-10261. [PMID: 1946446]
18. Lorand L. Transglutaminase mediated cross-linking of proteins and cell aging: the erythrocyte and lens models, In: Zappia V, editor. Advances in post-translational modifications ofproteins and aging. New York: Plenum Press; 1988. p. 79-94.
19. Lapko VN, Smith DL, Smith JB. In vivo carbamylation and acetylation of water soluble human lens alpha B-crystallinlysine 92. Protein Sci 2001; 10:1130-1136. [PMID: 11369851]
20. Gupta R, Srivastava OP. Deamidation affects structural and functional properties of human αA-crystallin and its oligomerization with αB-crystallin. J Biol Chem 2004; 279:44258-44269. [PMID: 15284238]a
21. Gupta R, Srivastava OP. Effect of deamidation of asparagines 146 on functional and structural properties of human lens αB-crystallin. Invest Ophthalmol Vis Sci 2004; 45:206-214. [PMID: 14691175]b
22. Lampi KJ, Amyx KK, Ahmann P, Steel EA. Deamidation in human lens βB2-crystallin destabilizes the dimmer. Biochemistry 2006; 45:3146-3153. [PMID: 16519509]
23. Hains PG, Truscott RJW. Post-translational modifications in the nuclear region of young, aged, and cataract human lenses. J Proteome Res 2007; 6:3935-3943. [PMID: 17824632]
24. Ma Z, Hanson SRA, Lampi KJ, David LL, Smith DL, Smith JB. Age-related changes in human lens crystallins identified by HPLC and mass spectrometry. Exp Eye Res 1998; 67:21-30. [PMID: 9702175]
25. Wilmarth PA, Tanner S, Dasari S, Nagalla SR, Riviere MA, Bafna V, Pevzner PA, David LL. Age-related changes in human crystallins determined from comparative analysis of post-translational modifications in young and aged lens: does deamidation contribute to crystalline insolubility? J Proteome Res 2006; 5:2554-2566. [PMID: 17022627]
26. Harrington V, McCall S, Huynh S, Srivastava K, Srivastava OP. Crystallins in water soluble-high molecular weight protein fractions and water insoluble protein fractions in aging and cataractous human lenses. Mol Vis 2004; 10:476-489. [PMID: 15303090]
27. Harrington V, Srivastava OP, Kirk M. Proteomic analysis of water insoluble proteins from normal and cataractous human lenses. Mol Vis 2007; 13:1680-1694. [PMID: 17893670]
28. Srivastava OP, Srivastava K. Existence of deamidated αBcrystallin fragments in normal and cataractous human lenses. Mol Vis 2003; 9:110-118. [PMID: 12707643]
29. Hanson SR, Hasan A, Smith DL, Smith JB. The major in vivo modifications of the human water-insoluble lens crystallins are disulfide bonds, deamidation, methionine oxidation and backbone cleavage. Exp Eye Res 2000; 71:195-207. [PMID: 10930324]
30. Lund AL, Smith JB, Smith DL. Modifications of the waterinsoluble human lens α-crystallins. Exp Eye Res 1996; 63:661-672. [PMID: 9068373]
31. Kim YH, Kapfe DM, Boekhorst J, Lubsen NH, Bachinger HP, Shearer TR, David LL, Feix JB, Lampi KJ. Deamidation, but not truncation decreases the urea stability of a lens structural protein, βB1-crystallin. Biochemistry 2002; 41:14076-14084. [PMID: 12437365]
32. Chaves JM, Srivastava K, Gupta R, Srivastava OP. Structural and functional roles of deamidation and/or truncation of Nor C-termini in human αA-crystallin. Biochemistry 2008; 47:10069-10083. [PMID: 18754677]
33. Banks RE, Dunn MJ, Forbes MA, Stanley A, Pappin D, Naven T, Gough M, Harnden P, Selby PJ. The potential use of laser capture microdissection to selectively obtain distinct populations of cells for proteomic analysis - preliminary findings. Electrophoresis 1999; 20:689-700. [PMID: 10344234]
34. Espina V, Wulfkuhle JD, Calvert VS, VanMeter A, Zhou W, Coukos G, Geho DH, Petricoin EF 3rd, Liotta LA. Lasercapture microdissection. Nat Protoc 2006; 1:586-603. [PMID: 17406286]
35. Kondo T HirohashiSApplication of highly sensitive fluorescent dyes (CyDye DIGE Fluor saturation dyes) to laser microdissection and two-dimensional difference gel electrophoresis (2D-DIGE) for cancer proteomics. Nat Protoc20061294056 17406554
36. Simone NL, Paweletz CP, Charboneau L, Petricoin EF 3rd, Liotta LA. Laser capture microdissection: beyond functional genomics to proteomics. Mol Diagn 2000; 5:301-307. [PMID: 11172494]a
37. Craven RA, Banks RE. Laser capture microdissection and proteomics: possibilities and limitation. Proteomics 2001; 1:1200-1204. [PMID: 11721632]
38. Mouledous L, Hunt S, Harcourt R, Harry J, Williams KL, Gutstein HB. Navigated laser capture microdissection as an alternative to direct histological staining for proteomic analysis of brain samples. Proteomics 2003; 3:610-615. [PMID: 12748941]
39. Simone NL, Bonner RF, Gillespie JW, Emmert-Buck MR,Liotta LA. Laser-capture microdissection: opening the microscopic frontier to molecular analysis. Trends Genet 1998; 14:272-276. [PMID: 9676529]
40. Simone NL, Remaley AT, Charboneau L, Petricoin EF 3rd, Glickman JW, Emmert-Buck MR, Fleisher TA, Liotta LA. Sensitive immunoassay of tissue cell proteins procured by laser capture microdissection. Am J Pathol 2000; 156:445-452. [PMID: 10666374]b
41. Lawrie LC, Curran S, McLeod HL, Fothergill JE, Murray GI. Application of laser capture microdissection and proteomics in colon cancer. Mol Pathol 2001; 54:253-258. [PMID: 11477141]
42. Grey AC, Schey KL. Distribution of bovine and rabbit lens α-crystallin products by MALDI imaging mass spectrometry. Mol Vis 2008; 14:171-179. [PMID: 18334935]
43. Lampi KJ, Ma Z, Hanson SRA, Azuma M, Shih M, Shearer TR, Smith DL, Smith JB, David LL. Age-related changes in human lens crystallins identified by two-dimensional electrophoresis and mass spectrometry. Exp Eye Res 1998; 67:31-43. [PMID: 9702176]
44. Craven RA, Totty N, Harnden P, Selby PJ, Banks RE. Laser capture microdissection and two-dimensional polyacrylamide gel electrophoresis. Evaluation of tissue preparation and sample limitations. Am J Pathol 2002; 160:815-822. [PMID: 11891180]
45. Colvis C, Garland D. Posttranslational modification of human αA-crystallin: correlation with electrophoretic migration. Arch Biochem Biophys 2002; 397:319-323. [PMID: 11795889]
46. Tannu NS, Hemby SE. Two-dimensional fluorescence difference gel electrophoresis for comparative proteomics profiling. Nat Protoc 2006; 1:1732-1742. [PMID: 17487156]
47. Laemmli UK. Cleavage of structural proteins during the assembly of the head of bacteriophage T4. Nature 1970; 227:680-685. [PMID: 5432063]
48. Maisel H, Goodman M. Analysis of cortical and nuclear lens proteins by a combination of paper and starch gel electrophoresis. Anat Rec 1965; 151:209-215. [PMID: 14278712]
49. Smithies O. Zone electrophoresis in starch gels: group variations in the serum proteins of normal human adults. Biochem J 1955; 61:629-41. [PMID: 13276348]
50. Gupta R, Srivastava K, Srivastava OP. Truncation of motifs III and IV in human lens βA3-crystallin destabilizes the structure. Biochemistry 2006; 45:9964-78. [PMID: 16906755]
51. Harms MJ, Wilmarth PA, Kapfer DM, Steel EA, David LL, Bachinger HP, Lampi KJ. Laser light-scattering evidence for an altered association of βB1-crystallin deamidated in the connecting peptide. Protein Sci 2004; 13:678-686. [PMID: 14978307]
52. Lapko VN, Purkis AG, Smith DL, Smith JB. Deamidation in human γS-crystallin from cataractous lenses is influenced by surface exposure. Biochemistry 2002; 41:8638-8648. [PMID: 12093281]
53. Lapko VN, Smith DL, Smith JB. Methylation and carbamylation of human gamma-crystallins. Protein Sci 2003; 12:1762-1774. [PMID: 12876325]
54. Shearer D, Ens W, Standing K, Valdimarsson G. Posttranslational modifications in lens fiber connexins identified by Off-Line-HPLC MALDI-Quadrupole Time-of-Flight mass spectrometry. Invest Ophthalmol Vis Sci 2008; 49:1553-1562. [PMID: 18385075]
55. Driessen HPC, Ramaekers FCS, Vree Egberts WTM, Dodemont HJ, de Jong WW, Tesser GI, Bloemendal H. The function of Nα-acetylation of the eye-lens crystallins. Eur J Biochem 1983; 136:403-406. [PMID: 6628390]
56. Wilmarth PA, Taube JR, Riviere MA, Duncan MK, David LL. Proteomic and sequence analysis of chicken lens crystallins reveals alternate splicing and translational forms of βB2 and βA2 crystallins. Invest Ophthalmol Vis Sci 2004; 45:2705-2715. [PMID: 15277495]
57. Lin PP, Barry RC, Smith DL, Smith JB. In vivo acetylation identified at lysine 70 of human lens αA-crystallin. Protein Sci 1998; 7:1451-1457. [PMID: 9655350]
58. Bhat SP, Spector A. A simple method to characterize gammacrystallin synthesized in vitro. Exp Eye Res 1984; 39:317-323. [PMID: 6209153]
59. MacCoss MJ, McDonald WH, Saraf A, Sadygov R, Clark JM, Tasto JJ, Gould KL, Wolters D, Washburn M, Weiss A, Clark JI, Yates JR 3rd. Shotgun identification of protein modifications from protein complexes and lens tissue. Proc Natl Acad Sci USA 2002; 99:7900-7905. [PMID: 12060738]
60. Spector A, Chiesa R, Sredy J, Garner W. cAMP-dependent phosphorylation of bovine lens α-crystallin. Proc Natl Acad Sci USA 1985; 82:4712-4716. [PMID: 2991889]