Quantitation of asparagine-101 deamidation from alpha-A crystallin during aging of the human lens

Current Eye Research

Volumn 17, Issue 3, 1998, Pages 247-250

  Takemoto, Larry J ^a  

^a KANSAS STATE UNIVERSITY   (United States)

Author keywords

Alpha A crystallin; Asparagine; Deamidation; Human ocular lens

Indexed keywords

ALPHA CRYSTALLIN; ASPARAGINE; SYNTHETIC PEPTIDE;

AGED; AGING; AMIDATION; ARTICLE; CONTROLLED STUDY; DEAMINATION; HUMAN; HUMAN TISSUE; INFANT; LENS; PRIORITY JOURNAL; PROTEIN PURIFICATION; REVERSED PHASE LIQUID CHROMATOGRAPHY;

ADOLESCENT; ADULT; AGED; AGING; ASPARAGINE; CHILD; CHILD, PRESCHOOL; CRYSTALLINS; DEAMINATION; HUMANS; INFANT; LENS, CRYSTALLINE; MIDDLE AGED;

EID: 0031909588     PISSN: 02713683     EISSN: None     Source Type: Journal    
DOI: 10.1076/ceyr.17.3.247.5218     Document Type: Article

References (14)

1. Horwitz, J. ( 1992) α-crystallin can function as a molecular chaperone. Proc. Natl. Acad. Sci. USA, 89, 10449-10453.
2. Boyle, D. and Takemoto, L. (1994) Characterization of the alpha-gamma and alpha-beta complex: evidence for an in vivo functional role of alpha-crystallin as a molecular chaperone. Exp. Eye Res. 58, 9-16.
3. Caspars, G.-J., Pennings, J. and de Jong, W. (1994) A partial cDNA sequence corrects the human αA-crystallin primary structure. Exp. Eye Res. 59, 125-126.
4. Kramps, J., de Jong, W., Wollensak, J. and Hoenders, H. (1978) The polypeptide chains of α-crystallin from old human eye lenses. Biochim. Biophys. Acta, 533, 487-495.
5. Lund, A., Smith, J. and Smith, D. (1996) Modifications of the water-insoluble human lens α-crystallins. Exp. Eye Res. 63, 661-672.
6. Takemoto, L. (1996) Increase in the intramolecular disulfide bonding of alpha-A crystallin during aging of the human lens. Exp. Eye Res. 63, 585-590.
7. Bradford, M. (1976) A rapid and sensitive method for quantitation of microgram quantities of protein utilizing the principle of protein-dye binding. Analy. Biochem. 72, 248-254.
8. Wright, H. (1991) Nonenzymatic deamidation of asparaginyl and glutaminyl residues in proteins. Crit. Rev. Biochem. Mol. Biol. 26, 1-52.
9. Robinson, A., McKerrow, J. and Cary, P. (1970) Controlled deamidation of peptides and proteins: an experimental hazard and possible biological timer. Proc. Natl. Acad. Sci. USA, 66, 753-755.
10. Flatau, G., Lemichez, E., Gauthier, M., Chardin, P., Paris, S., Florentini, C. and Boquet, P. (1997) Toxin-induced activation of the G protein p21 Rho by deamidation of glutamine. Nature, 387, 729-733.
11. Gracy, R., Yuksel, K. and Gomez-Puyou, A. (1994) In Deamidation and Isoaspartate Formation in Peptides and Proteins (Ed. Aswad, D.). Pp. 133-155. CRC Press, Boca Raton, FL, USA.
12. Voorter, C., de Haard-Hoekman, W., van den Oetelaar, P., Bloemendal, H. and de Jong, W. (1988) Spontaneous peptide bond cleavage in aging α-crystallin through a succinimide intermediate. J. Biol. Chem. 263, 19020-19023.
13. Yang, Z., Chamorro, M., Smith, D. and Smith, J. (1994) Identification of the major components of the high molecular weight crystalline from old human lenses. Curr. Eye Res. 13, 415-421.
14. Jedziniak, J., Kinoshita, J., Yates, E., Hocker, L. and Benedek, G. (1973) On the presence and mechanism of formation of heavy molecular weight aggregates in human normal and cataractous lenses. Exp. Eye Res. 15, 185-192.