In vivo acetylation identified at lysine 70 of human lens αA- crystallin

Protein Science

Volumn 7, Issue 6, 1998, Pages 1451-1457

  Lin, Peiping P ^a,b   Barry, Richard C ^a   Smith, David L ^a   Smith, Jean B ^a  

^a UNIVERSITY OF NEBRASKA LINCOLN   (United States)

^b MDS Inc   (Canada)

Author keywords

Acetylation; Ion exchange; Lens crystallins; Mass spectrometry

Indexed keywords

ALPHA CRYSTALLIN; CHAPERONE; LENS PROTEIN; LYSINE;

ACETYLATION; ADULT; AGED; ANION EXCHANGE CHROMATOGRAPHY; ARTICLE; CATARACT; CONFORMATIONAL TRANSITION; HIGH PERFORMANCE LIQUID CHROMATOGRAPHY; HUMAN; IN VIVO STUDY; MASS SPECTROMETRY; NORMAL HUMAN; PRIORITY JOURNAL; PROTEIN PROCESSING;

EID: 0031745913     PISSN: 09618368     EISSN: None     Source Type: Journal    
DOI: 10.1002/pro.5560070622     Document Type: Article

References (43)

1. Abraham EC, Cherian M, Smith JB. 1994. Site selectivity in the glycation of αA-and αB-crystallins by glucose. Biochem Biophys Res Commun 201:1451-1456.
2. Ajiboye R, Harding JJ. 1989. The non-enzymic glycosylation of bovine lens proteins by glucosamine and its inhibition by aspirin, ibuprofen and glutathione. Exp Eye Res 49:31-41.
3. Bensch KG, Fleming JE, Lohmann W. 1985. The role of ascorbic acid in senile cataract. Proc Natl Acad Sci USA 82:7193-7196.
4. Beswick HT, Harding JJ. 1984. Conformational changes induced in bovine lens α-crystallin by carbamylation. Biochem J 223:221-227.
5. Beswick HT, Harding JJ. 1987. High-molecular-weight crystallin aggregate formation resulting from non-enzymic carbamylation of lens crystallins: Relevance to cataract formation. Exp Eye Res 45:569-578.
6. Biemann K. 1990. Sequencing of peptides by tandem mass spectrometry and high-energy collision-induced dissociation. In: McCloskey JA, ed. Methods in enzymology. San Diego, CA: Academic Press. pp 455-479.
7. Blakytny R, Harding JJ. 1992. Prevention of cataract in diabetic rats by aspirin, paracetamol (acetaminophen) and ibuprofen. Exp Eye Res 54:509-518.
8. Crompton M, Rixon KC, Harding JJ. 1985. Aspirin prevents carbamylation of soluble lens proteins and prevents cyanate-induced phase separation opacities in vitro: A possible mechanism by which aspirin could prevent cataract. Exp Eye Res 40:297-311.
9. Das KP, Surewicz WK. 1995. Temperature-induced exposure of hydrophobic surfaces and its effect on the chaperone activity of α-crystallin. FEBS Lett 369:321-325.
10. Datiles MB, Schumer DJ, Zigler JS, Russell P, Anderson L, Garland D. 1992. Two-dimensional gel electrophoretic analysis of human lens proteins. Curr Eye Res 11:6690-677.
11. Dunn AJ, Patrick JS, Thorpe SR, Baynes JW. 1989. Oxidation of glycated proteins: Age-dependent accumulation of Ne-(carboxymethyl)lysine in lens proteins. Biochemistry 28:9464-9468.
12. Dyer DG, Blackledge JA, Thorpe SR, Baynes JW. 1991. Formation of pentosidine during nonenzymatic browning of proteins by glucose. J Biol Chem 266:11654-11660.
13. Garland DL, Duglas-Tabor Y, Jimenez-Asensio J, Datiles MB, Magno B. 1996. The nucleus of the human lens: Demonstration of a highly characteristic protein pattern by two-dimensional electrophoresis and introduction of a new method of lens dissection. Exp Eye Res 62:285-291.
14. Groenen PJTA, van Dongen MJP, Voorter CEM, Bloemendal H, de Jong WW. 1993. Age-dependent deamidation of αB-crystallin. FEBS Lett 322:69-72.
15. Gu W, Roeder RG. 1997. Activation of p53 sequence-specific DNA binding by acetylation of the p53 C-terminal domain. Cell 90:595-606.
16. Harding JJ. 1972. Conformational changes in human lens proteins in cataract. Biochem J 129:97-100.
17. Harding JJ. 1973. Disulphide cross-linked protein of high molecular weight in human cataractous lens. Exp Eye Res 17:377-383.
18. Harding JJ, Egerton M, Harding RS. 1989. Protection against cataract by aspirin, paracetamol and ibuprofen. Acta Ophthalmol 67:518-524.
19. Harding JJ, Rixon KC. 1980. Carbamylation of lens proteins: A possible factor in cataractogenesis in some tropical countries. Exp Eye Res 31:567-571.
20. Hasan A, Smith JB, Qin W, Smith DL. 1993. The reaction of bovine lens αA-crystallin with aspirin. Exp Eye Res 57:29-35.
21. Horwitz J. 1992. α-Crystallin can function as a molecular chaperone. Proc Natl Acad Sci USA 89:10449-10453.
22. Huby R, Harding JJ. 1988. Non-enzymic glycosylation (glycation) of lens proteins by galactose and protection by aspirin and reduced glutathione. Exp Eye Res 47:53-59.
23. Lampi KJ, Ma Z, Hanson SRA, Azuma M, Shih M, Shearer TR, Smith DL, Smith JB, David LL. 1998. Age-related changes in human lens crystallins identified by two-dimensional electrophoresis and mass spectrometry. Exp Eye Res. In press.
24. Lund AL, Smith JB, Smith DL. 1996. Modifications of the water-insoluble human lens α-crystallins. Exp Eye Res 63:661-672.
25. Ma Z, Hanson SRA, Lampi KJ, David LL, Smith DL, Smith JB. 1998. Age-related changes in human lens crystallins identified by HPLC and mass spectrometry. Exp Eye Res. In press.
26. Miesbauer LR, Zhou X, Yang Z, Yang Z, Sun Y, Smith DL, Smith JB. 1994. Post-translational modifications of the water-soluble human lens crystallins from young adults. J Biol Chem 269:12494-12502.
27. Muchowski PJ, Bassuk JA, Lubsen NH, Clark JI. 1997. Human αB-crystallin: Small heat shock protein and molecular chaperone. J Biol Chem 272:2578-2582.
28. Nagaraj RH, Monnier VM. 1992. Isolation and characterization of a blue fluorophore from human eye lens crystallins: In vitro formation from Maillard reaction with ascorbate and ribose. Biochim Biophys Acta 1116:34-42.
29. Nagaraj RH, Sell DR, Prabhakaram M, Ortwerth BJ, Monnier VM. 1991. High correlation between pentosidine protein crosslinks and pigmentation implicates ascorbate oxidation in human lens senescence and cataractogenesis. Proc Natl Acad Sci USA 88:10257-10261.
30. Ortwerth BJ, Feather MS, Olesen PR. 1988. The precipitation and cross-linking of lens crystallins by ascorbic acid. Exp Eye Res 47:155-168.
31. Ortwerth BJ, Slight SH, Prabhakaram M, Sun Y, Smith JB. 1992. Site-specific glycation of lens crystallins by ascorbic acid. Biochim Biophys Acta 1117:207-215.
32. Prabhakaram M, Ortwerth BJ. 1992. The glycation and cross-linking of isolated lens crystallins by ascorbic acid. Exp Eye Res 55:451-459.
33. Qin W, Smith JB, Smith DL. 1993. Reaction of aspirin with cysteinyl residues of lens γ-crystallins: A mechanism for the proposed anti-cataract effect of aspirin. Biochim Biophys Acta 1181:103-110.
34. Rao GN, Cotlier E. 1988. Aspirin prevents the nonenzymatic glycosylation and carbamylation of the human eye lens crystallins in vitro. Biochem Biophys Res Commun 151:991-996.
35. Rao GN, Lardis MP, Cotlier E. 1985. Acetylation of lens crystallins: A possible mechanism by which aspirin could prevent cataract formation. Biochem Biophys Res Commun 128:1125-1132.
36. Raza K, Harding JJ. 1991. Non-enzymic modification of lens proteins by glucose and fructose: Effects of ibuprofen. Exp Eye Res 52:205-212.
37. Riley ML, Harding JJ. 1993. The reaction of malondialdehyde with lens proteins and the protective effect of aspirin. Biochim Biophys Acta 1158:107-112.
38. Riley ML, Harding JJ. 1995. The reaction of methylglyoxal with human and bovine lens proteins. Biochim Biophys Acta 1270:36-43.
39. Roepstorff P, Fohlman J. 1984. Proposal for a common nomenclature for sequence ions in mass spectra of peptides. Biomed Mass Spectrom 11:601.
40. Smith JB, Liu Y, Smith DL. 1996. Identification of possible regions of chaperone activity in lens α-crystallins. Exp Eye Res 63:125-128.
41. Swamy MS, Abraham EC. 1989. Inhibition of lens crystallin glycation and high molecular weight aggregate formation by aspirin in vitro and in vivo. Invest Ophthalmol Vis Sci 30:1120-1126.
42. Swamy MS, Tsai C, Abraham A, Abraham EC. 1993. Glycation mediated lens crystallin aggregation and cross-linking by various sugars and sugar phosphates in vitro. Exp Eye Res 56:177-185.
43. Takemoto LJ. 1996. Differential phosphorylation of α-A crystallin in human lens of different age. Exp Eye Res 62:499-504.