A novel method of protein extraction from yeast using ionic liquid solution

Talanta

Volumn 81, Issue 4-5, 2010, Pages 1861-1864

  Ge, Liya ^a   Wang, Xiao Tao ^b   Tan, Swee Ngin ^a   Tsai, Heng Hang ^c   Yong, Jean W H ^a   Hua, Lin ^c  

^a NANYANG TECHNOLOGICAL UNIVERSITY   (Singapore)

^b UNIVERSITY COLLEGE DUBLIN   (Ireland)

^c GENOME INSTITUTE OF SINGAPORE   (Singapore)

Author keywords

2 DE; Ionic liquid; Protein extraction; SDS PAGE; Western blotting; Yeast

Indexed keywords

ELECTROPHORESIS; IONIC LIQUIDS; PROTEINS; SODIUM DODECYL SULFATE; SULFUR COMPOUNDS; YEAST;

2-DE; 2-DIMENSIONAL GEL ELECTROPHORESIS; BIOLOGICAL FUNCTIONS; EXTRACTION EFFICIENCIES; PROTEIN EXTRACTION; SDS PAGE; SODIUM DODECYL SULFATE-POLYACRYLAMIDE GEL ELECTROPHORESIS; WESTERN BLOTTING;

EXTRACTION;

FUNGAL PROTEIN; ION; IONIC LIQUID; WATER;

CHEMICAL ANALYSIS; CHEMISTRY; FUNGUS; ISOLATION AND PURIFICATION; MECHANICAL STRESS; POLYACRYLAMIDE GEL ELECTROPHORESIS; PROCEDURES; TWO DIMENSIONAL GEL ELECTROPHORESIS; WESTERN BLOTTING;

BLOTTING, WESTERN; CHEMISTRY TECHNIQUES, ANALYTICAL; ELECTROPHORESIS, GEL, TWO-DIMENSIONAL; ELECTROPHORESIS, POLYACRYLAMIDE GEL; FUNGAL PROTEINS; FUNGI; IONIC LIQUIDS; IONS; STRESS, MECHANICAL; WATER;

EID: 77952106011     PISSN: 00399140     EISSN: None     Source Type: Journal    
DOI: 10.1016/j.talanta.2010.02.034     Document Type: Article

References (17)

1. Wohlschlegel J.A., and Yates J.R. Nature 425 (2003) 671-672
2. Ghaemmaghami S., Huh W.K., Bower K., Howson R.W., Belle A., et al. Nature 425 (2003) 737-741
3. Ganeva V., and Galutzov B. FEMS Microbiol. Lett. 174 (1999) 279-284
4. Stowers C.C., and Boczko E.M. Yeast 24 (2007) 533-541
5. Kushnirov V.V. Yeast 16 (2000) 857-860
6. Matsuo Y., Asakawa K., Toda T., and Katayama S. Biosci. Biotechnol. Biochem. 70 (2006) 1992-1994
7. Earle M.J., and Seddon K.R. Pure Appl. Chem. 72 (2000) 1391-1398
8. Cull S.G., Holbrey J.D., Vargas-Mora V., Seddon K.R., and Lye G.J. Biotechnol. Bioeng. 692 (2000) 227-233
9. Huddleston J.G., Willauer H.D., Swatloski R.P., Visser A.E., and Rogers R.D. Chem. Commun. 16 (1998) 1765-1766
10. Howarth J. Tetrahedron Lett. 41 (2000) 6627-6629
11. Seddon K.R. J. Chem. Technol. Biotechnol. 68 (1997) 351-356
12. Hussey C.L. Pure Appl. Chem. 60 (1988) 1763-1772
13. Cheng D.-H., Chen X.-W., Shu Y., and Wang J.-H. Talanta 75 (2008) 1270-1278
14. Lerouxel O., Cavalier D.M., Liepman A.H., and Keegstra K. Curr. Opin. Plant Biol. 9 (2006) 621-630
15. Kadokawa J.I., Murakami M.A., and Kaneko Y. Carbohydr. Res. 343 (2008) 769-772
16. Fukaya Y., Sugimoto A., and Ohno H. Biomacromolecules 7 (2006) 3295-3297
17. Mrsa V., Seidl T., Gentzsch M., and Tanner W. Yeast 13 (1997) 1145-1154