Expression and purification of recombinant human α1-proteinase inhibitor and its single amino acid substituted variants in Escherichia coli for enhanced stability and biological activity

Journal of Biotechnology

Volumn 147, Issue 1, 2010, Pages 64-72

  Agarwal, Saurabh ^a   Jha, Shweta ^a   Sanyal, Indraneel ^a   Amla, D V ^a  

^a CSIR NATIONAL BOTANICAL RESEARCH INSTITUTE   (India)

Author keywords

E. coli expression; MBP fusion protein; Modified synthetic gene; Recombinant 1 proteinase inhibitor; Site specific mutation; Thermostability

Indexed keywords

E. COLI; FUSION PROTEINS; PROTEINASE INHIBITORS; SITE-SPECIFIC; SYNTHETIC GENES;

AFFINITY CHROMATOGRAPHY; AMINO ACIDS; CELL CULTURE; ESCHERICHIA COLI; ORGANIC ACIDS; PROTEINS; PURIFICATION;

GENE EXPRESSION;

ALPHA 1 ANTITRYPSIN; ELASTASE; MALTOSE; RECOMBINANT ALPHA 1 ANTITRYPSIN; RECOMBINANT PROTEIN; TRANSFER RNA; UNCLASSIFIED DRUG;

AFFINITY CHROMATOGRAPHY; AMINO ACID SUBSTITUTION; AMINO TERMINAL SEQUENCE; ARTICLE; BACTERIUM CULTURE; BIOLOGICAL ACTIVITY; CONTROLLED STUDY; DRUG DESIGN; DRUG SYNTHESIS; ENZYME ACTIVITY; ENZYME STABILITY; ESCHERICHIA COLI; GENE EXPRESSION; GENETIC VARIABILITY; MASS SPECTROMETRY; MOLECULAR WEIGHT; NONHUMAN; NUCLEOTIDE SEQUENCE; PRIORITY JOURNAL; PROTEIN AGGREGATION; PROTEIN BINDING; PROTEIN DEGRADATION; PROTEIN EXPRESSION; PROTEIN GLYCOSYLATION; PROTEIN PURIFICATION; PROTEIN STRUCTURE; SOLUBILITY; SWINE; CHEMISTRY; GENE VECTOR; GENETICS; HUMAN; ISOLATION AND PURIFICATION; METABOLISM; MUTATION; PROTEIN TERTIARY STRUCTURE; TEMPERATURE;

ALPHA 1-ANTITRYPSIN; AMINO ACID SUBSTITUTION; ENZYME STABILITY; ESCHERICHIA COLI; GENETIC VECTORS; HUMANS; MUTATION; PROTEIN STRUCTURE, TERTIARY; RECOMBINANT PROTEINS; SPECTROMETRY, MASS, MATRIX-ASSISTED LASER DESORPTION-IONIZATION; TEMPERATURE;

BACTERIA (MICROORGANISMS); ESCHERICHIA COLI;

EID: 77951975720     PISSN: 01681656     EISSN: None     Source Type: Journal    
DOI: 10.1016/j.jbiotec.2010.03.008     Document Type: Article

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