Cell contraction forces in scaffolds with varying pore size and cell density

Biomaterials

Volumn 31, Issue 18, 2010, Pages 4835-4845

  Corin, Karolina A ^a   Gibson, Lorna J ^a  

^a Massachusetts Institute of Technology Cambridge   (United States)

Author keywords

Cell density; Collagen GAG scaffolds; Contraction; Pore size

Indexed keywords

CELL CONTRACTION; CELL DENSITY; CELL FORCES; CONTRACTILE BEHAVIOR; CONTRACTILE FORCE; CONTRACTION; ELASTOMERIC FOAMS; FINITE ELEMENT ANALYSIS; INDIVIDUAL CELLS; NORMAL HUMAN DERMAL FIBROBLASTS; NORMAL TISSUE; NUMERICAL MODELS; OPEN-CELL; RELATIVE DENSITY; SCAR FORMATION; SCAR TISSUES; SOLID SUBSTRATES; TISSUE ENGINEERING SCAFFOLD; WOUND HEALING;

BIOMATERIALS; CELL CULTURE; COLLAGEN; FIBROBLASTS; FINITE ELEMENT METHOD; PHYSIOLOGICAL MODELS; PORE SIZE; STRAIN; STRUTS; TISSUE; TISSUE ENGINEERING;

SCAFFOLDS;

COLLAGEN; GLYCOSAMINOGLYCAN; TISSUE SCAFFOLD;

ARTICLE; CELL DENSITY; CONTRACTION STRESS; CONTROLLED STUDY; FINITE ELEMENT ANALYSIS; HUMAN; HUMAN CELL; MATHEMATICAL MODEL; POROSITY; PRIORITY JOURNAL; RELATIVE DENSITY; SCAR FORMATION; SKIN FIBROBLAST; STRESS STRAIN RELATIONSHIP; TISSUE ENGINEERING; TISSUE REGENERATION; WOUND HEALING;

CELL COUNT; CELL PROLIFERATION; CELL SURVIVAL; COLLAGEN; DERMIS; FIBROBLASTS; HUMANS; MODELS, BIOLOGICAL; POROSITY; TISSUE ENGINEERING; TISSUE SCAFFOLDS;

EID: 77951975692     PISSN: 01429612     EISSN: None     Source Type: Journal    
DOI: 10.1016/j.biomaterials.2010.01.149     Document Type: Article

References (42)

1. Yannas I.V. Tissue and organ regeneration in adults 2001, Springer-Verlag, New York.
2. Chamberlain L.J., Yannas I.V., Hsu H., Spector M. Connective tissue response to tubular implants for peripheral nerve regeneration: the role of myofibroblasts. J Comp Neurol 2000, 417:415-430.
3. Murray M.M., Martin S.D., Martin T.L., Spector M. Histological changes in the human anterior cruciate ligament after rupture. J Bone Joint Surg Am 2000, 82-A:1387-1397.
4. Desmouliere A., Gabbiani G. The role of the myofibroblast in wound healing and fibrocontractive diseases. The molecular and cellular biology of wound repair 1996, Plenum Press, New York. R.A.F. Clark (Ed.).
5. Hsu W., Spilker M.H., Yannas I.V., Rubin P.A.D. Inhibition of conjuctival scarring and contraction by a porous collagen-glycosaminoglycan implant. Invest Opthalmol Vis Sci 2000, 41:2404-2411.
6. Burton K., Taylor D.L. Traction forces of cytokinesis measured with optically modified elastic substrata. Nature 1997, 385(6615):450-454.
7. Harris A.K., Wild P., Stopak D. Silicone rubber substrata: a new wrinkle in the study of cell locomotion. Science 1980, 208(4440):177-179.
8. Harris A.K., Stopak D., Wild P. Fibroblast traction as a mechanism for collagen morphogenesis. Nature 1981, 290(5803):249-251.
9. Beningo K.A., Wang Y.L. Flexible substrata for the detection of cellular traction forces. Trends Cell Biol 2002, 12(2):79-84.
10. Dembo M., Wang Y.L. Stresses at the cell-to-substrate interface during locomotion of fibroblasts. Biophys J 1999, 76:2307-2316.
11. Lee J., Leonard M., Oliver T., Ishihara A., Jacobson K. Traction forces generated by locomoting keratocytes. J Cell Biol 1994, 127(6,2):1957-1964.
12. Wang N., Naruse K., Stamenovic D., Fredberg J.J., Mijailovich S.M., Tolic-Norrelykke I.M., et al. Mechanical behavior in living cells consistent with the tensegrity model. Proc Natl Acad Sci U S A 2001, 98(14):7765-7770.
13. Wang N., Ostuni E., Whitesides G.M., Ingber D.E. Micropatterning tractional forces in living cells. Cell Motil Cytoskeleton 2002, 52:97-106.
14. Wrobel L.K., Fray T.R., Molloy J.E., Adams J.J., Armitage M.P., Sparrow J.C. Contractility of single human dermal myofibroblasts and fibroblasts. Cell Motil Cytoskeleton 2002, 52:82-90.
15. Balaban N.Q., Schwarz U.S., Riveline D., Goichberg P., Tzur G., Sabanay I., et al. Force and focal adhesion assembly: a close relationship studied using elastic micropatterned substrates. Nat Cell Biol 2001, 3:466-472.
16. Galbraith C.G., Sheetz M.P. A micromachined device provides a new bend on fibroblast traction forces. Proc Natl Acad Sci U S A 1997, 94:9114-9118.
17. Galbraith C.G., Sheetz M.P. Keratocytes pull with similar forces on their dorsal and ventral surfaces. J Cell Biol 1999, 147(6):1313-1323.
18. Lemmon C.A., Sniadecki N.J., Ruiz S.A., Tan J.L., Romer L.H., Chen C.S. Shear force at the cell-matrix interface: enhanced analysis for microfabricated post array detectors. Mech Chem Biosyst 2005, 2(1):1-16.
19. Tan J.L., Tien J., Pirone D.M., Gray D.S., Bhadriraju K., Chen C.S. Cells lying on a bed of microneedles: an approach to isolate mechanical force. Proc Natl Acad Sci U S A 2003, 100(4):1484-1489.
20. Zhao Y., Zhang X. Cellular mechanics study in cardiac myocytes using PDMS pillars array. Sens Actuators A Phys 2006, 125:398-404.
21. Park J., Ryu J., Choi S.K., Seo E., Cha J.M., Ryu S., et al. Real-time measurement of the contractile forces of self-organized cardiomyocytes on hybrid biopolymer microcantilevers. Anal Chem 2005, 77:6571-6580.
22. Sun M., Graham J.S., Hegediis B., Marga F., Zhang Y., Forgacs G., et al. Multiple membrane tethers probed by atomic force microscopy. Biophys J 2005, 89:4320-4329.
23. Yamamoto A., Mishima S., Maruyama N., Sumita M. A new technique for direct measurement of the shear force necessary to detach a cell from a material. Biomaterials 1998, 19(7-9):871-879.
24. Brown R.A., Prajapati R., McGrouther D.A., Yannas I.V., Eastwood M. Tensional homeostasis in dermal fibroblasts: mechanical responses to mechanical loading in three-dimensional substrates. J Cell Physiol 1998, 175:323-332.
25. Eastwood M., Porter R., Khan U., McGrouther G., Brown R. Quantitative analysis of collagen gel contractile forces generated by dermal fibroblasts and the relationship to cell morphology. J Cell Physiol 1996, 166:33-42.
26. Freyman T.M., Yannas I.V., Yokoo R., Gibson L.J. Fibroblast contraction of a collagen-GAG matrix. Biomaterials 2001, 22(21):2883-2891.
27. Freyman T.M., Yannas I.V., Yokoo R., Gibson L.J. Fibroblast contractile force is independent of the stiffness which resists the contraction. Exp Cell Res 2002, 272(2):153-162.
28. Jenkins G., Redwood K.L., Meadows L., Green M.R. Effect of gel re-organization and tensional forces on α2β1 integrin levels in dermal fibroblasts. Eur J Biochem 1999, 263:93-103.
29. Kolodney M.S., Wysolmerski R.B. Isometric contraction by fibroblasts and endothelial cells in tissue culture: a quantitative study. J Cell Biol 1992, 117:73-82.
30. Sethi K.K., Yannas I.V., Mudera V., Eastwood M., McFarland C., Brown R.A. Evidence for sequential utilization of fibronectin, vitronectin, and collagen during fibroblast-mediated collagen contraction. Wound Repair Regen 2002, 10(6):397-408.
31. Talas G., Adams T.S.T., Eastwood M., Rubio G., Brown R.A. Phenytoin reduces the contraction of recessive dystrophic epidermolysis bullosa fibroblast populated collagen gels. Int J Biochem Cell Biol 1997, 29(1):261-270.
32. Harley B.A., Freyman T.M., Wong M.Q., Gibson L.J. A new technique for calculating individual dermal fibroblast contractile forces generated within collagen-GAG scaffolds. Biophys J 2007, 93:2911-2922.
33. Zhu H.X., Knott J.F., Mills N.J. Analysis of the elastic properties of open-cell foams with tetrakaidecahedral cells. J Mech Phys Solids 1997, 45:319-343.
34. Harley B.A., Leung J.H., Silva E.C.C.M., Gibson L.J. Mechanical characterization of collagen-glycoaminoglycan scaffolds. Acta Biomater 2007, 3:463-474.
35. Gibson L.J., Ashby M.F. Cellular solids: structures and properties 1997, Cambridge University Press, New York. 2nd ed.
36. Gong L., Kyriakides S., Jang W.J. Compressive response of open-cell foams: part I: morphology and elastic properties. Int J Solids Struct 2005, 42:1355-1379.
37. Warren W.E., Kraynik A.M. Linear elastic behavior of a low-density kelvin foam with open cells. J Appl Mech 1997, 64:787-794.
38. O'Brien F.J., Harley B.A., Yannas I.V., Gibson L.J. The effect of pore size on cell adhesion in collagen-GAG scaffolds. Biomaterials 2005, 26:433-441.
39. O'Brien F.J., Harley B.A., Yannas I.V., Gibson L.J. Influence of freezing rate on pore structure in freeze-dried collagen-GAG scaffolds. Biomaterials 2004, 25(6):1077-1086.
40. Harley B.A. Cell-matrix interactions: collagen-GAG scaffold fabrication, characterization, and measurement of cell migratory and contractile behavior via confocal microscopy 2006, [D. Sci], Mechanical Engineering, Massachusetts Institute of Technology, Cambridge.
41. Freyman T.M., Yannas I.V., Pek Y.S., Yokoo R., Gibson L.J. Micromechanics of fibroblast contraction of a collagen-GAG matrix. Exp Cell Res 2001, 269:140-153.
42. Kanungo BP, Gibson LJ. Density-property relationships in collagen-glycosaminoglycan scaffolds. Submitted to Acta Biomater; 2009.