PH-dependent self-association of zinc-free insulin characterized by concentration-gradient static light scattering

Biophysical Chemistry

Volumn 148, Issue 1-3, 2010, Pages 28-33

  Attri, Arun K ^a   Fernández, Cristina ^a   Minton, Allen P ^a  

^a NATIONAL INSTITUTE OF DIABETES AND DIGESTIVE AND KIDNEY DISEASES   (United States)

Author keywords

Insulin; Self association equilibria; Static light scattering

Indexed keywords

BOVINE INSULIN; ZINC;

ANALYTIC METHOD; ARTICLE; CALCULATION; CHEMICAL PARAMETERS; CONCENTRATION (PARAMETERS); CONCENTRATION GRADIENT STATIC LIGHT SCATTERING; CONTROLLED STUDY; HOMEOSTASIS; LIGHT SCATTERING; MATHEMATICAL MODEL; MOLECULAR WEIGHT; NONHUMAN; PH; PRIORITY JOURNAL; QUANTITATIVE ANALYSIS; SEDIMENTATION RATE;

ANIMALS; CATTLE; HYDROGEN-ION CONCENTRATION; INSULIN; LIGHT; MODELS, CHEMICAL; MOLECULAR WEIGHT; PROTEIN BINDING; SCATTERING, RADIATION; ZINC;

EID: 77951975291     PISSN: 03014622     EISSN: None     Source Type: Journal    
DOI: 10.1016/j.bpc.2010.02.002     Document Type: Article

References (31)

1. Hefford M.A., Oda G., Kaplan H. Structure-function relationships in the free insulin monomer. Biochem. J. 1986, 237:663-668.
2. Pekar A.H., Frank B.H. Conformation of proinsulin. A comparison of insulin and proinsulin self-association at neutral pH. Biochemistry 1972, 11:4013-4016.
3. Jeffrey P.D., Coates J.H. An equilibrium ultracentrifugation study of the effect of ionic strength on the self-association of bovine insulin. Biochemistry 1966, 5:3820-3824.
4. Mark A.E., Nichol L.W., Jeffrey P.D. The self-association of zinc-free bovine insulin. A single model based on interactions in the crystal that describes the association pattern in solution at pH 2, 7 and 10. Biophys. Chem. 1987, 27:103-117.
5. Jeffrey P.D., Milthorpe B.K., Nichol L.W. Polymerization pattern of insulin at pH 7.0. Biochemistry 1976, 15:4660-4665.
6. Whittingham J.L., Scott D.J., Chance K., Wilson A., Finch J., Brange J., Dodson G.G. Insulin at pH 2: structural analysis of conditions promoting insulin fiber formation. J. Mol. Biol. 2002, 318:479-490.
7. Dathe M., Gast K., Zirwer D., Welfle H., Mehlis B. Insulin aggregation in solution. Int. J. Pept. Protein Res. 1990, 36:344-349.
8. Hvidt S. Insulin association in neutral solutions studied by light scattering. Biophys. Chem. 1991, 39:205-213.
9. Bohidar H.B., Geissler E. Static and dynamic light scattering from dilute insulin solutions. Biopolymers 1984, 23:2407-2417.
10. Kadima W., Ogendal L., Bauer R., Kaarsholm N., Brodersen K., Hansen J.F., Porting P. The influence of ionic strength and pH on the aggregation properties of zinc-free insulin studied by static and dynamic laser light scattering. Biopolymers 1993, 33:1643-1657.
11. Pedersen J.S., Hansen S., Bauer R. The aggregation behavior of zinc-free insulin studied by small-angle neutron scattering. Eur. Biophys. J. 1994, 22:379-389.
12. Nielsen L., Khurana R., Coats A., Frokjaer S., Brange J., Vyas S., Uversky V.N., Fink A.L. Effect of environmental factors on kinetics of insulin fibril formation: elucidation of molecular mechanism. Biochemistry 2001, 40:6036-6046.
13. Uversky V.N., Garriques L.N., Millett I.S., Frokjaer S., Brange J., Doniach S., Fink A.L. Prediction of the association state of insulin using spectral parameters. J. Pharm. Sci. 2003, 92:847-858.
14. Hansen J.F. The self-association of zinc-free human insulin and insulin analogue B13-glutamine. Biophys. Chem. 1991, 39:107-110.
15. Nettleton E.J., Tito P., Sunde M., Bouchard M., Dobson C.M., Robinson C.V. Characterization of the oligomeric states of insulin in self-assembly and amyloid fibril formation by mass spectrometry. Biophys. J. 2000, 79:1053-1065.
16. Bocian W., Sitkowski J., Tarnowska A., Bednarek E., Kawecki R., Koźmiński W., Kozerski L. Direct insight into insulin aggregation by 2D NMR complemented by PFGSE NMR. Proteins 2008, 71:1057-1065.
17. Pocker Y., Biswas S.B. Self-association of insulin and the role of hydrophobic bonding: a thermodynamic model of insulin dimerization. Biochemistry 1981, 20:4354-4361.
18. Mark A.E., Jeffrey P.D. The self-association of zinc-free bovine insulin: four model pattern and their significance. Biol. Chem. 1990, 371:1165-1174.
19. Attri A.K., Minton A.P. New methods for measuring macromolecular interactions in solution via static light scattering: basic methodology and application to nonassociating and self-associating proteins. Anal. Biochem. 2005, 337:103-110.
20. Attri A.K., Minton A.P. Composition gradient static light scattering: a new technique for rapid detection and quantitative characterization of reversible macromolecular hetero-associations in solution. Anal. Biochem. 2005, 346:132-138.
21. Kameyama K., Minton A.P. Rapid quantitative characterization of protein interactions by composition gradient static light scattering. Biophys. J. 2006, 90:2164-2169.
22. Some D., Hanlon A., Sokolov K. Characterizing protein-protein interactions via static light scattering: reversible heteroassociation. Am. Biotechnol. Lab. 2008, 26:18-20.
23. Feingold V., Jenkins A.B., Kraegen E.W. Effect of contact material on vibration-induced insulin aggregation. Diabetologia 1984, 27:373-378.
24. Sluzky V., Tamada J.A., Klibanov A.M., Langer R. Kinetics of insulin aggregation in aqueous solutions upon agitation in the presence of hydrophobic surfaces. Proc. Natl. Acad. Sci. U. S. A. 1991, 88:9377-9381.
25. Markussen J. Structural changes involved in the folding of proinsulin. Int. J. Protein Res. 1971, 3:201-207.
26. Perlmann G.E., Longsworth L.G. The specific refractive increment of some purified proteins. J. Am. Chem. Soc. 1948, 70:2719-2724.
27. Stacey K.A. Light Scattering in Physical Chemistry (Chapter 2) 1956, Academic Press, New York.
28. Weiss M.A., Hua Q.X., Lynch C.S., Frank B.H., Shoelson S.E. Heteronuclear 2D NMR studies of an engineered insulin monomer: assignment and characterization of the receptor-binding surface by selective 2H and 13C labeling with application to protein design. Biochemistry 1991, 30:7373-7389.
29. Dunn M.F. Zinc-ligand interactions modulate assembly and stability of insulin hexamer-a review. Biometals 2005, 18:295-303.
30. Howlett G.J., Minton A.P., Rivas G. Analytical ultracentrifugation for the study of protein association and assembly. Curr. Opin. Chem. Biol. 2006, 10:430-436.
31. A.K. Attri, C. Fernandez, A.P. Minton, Biophys. Chem. (in press).