Ruminal fermentation, microbial growth and amino acid flow in single-flow continuous culture fermenters fed a diet containing olive leaves

Journal of Animal Physiology and Animal Nutrition

Volumn 94, Issue 2, 2010, Pages 227-236

  Molina Alcaide, E ^a   Martin Garcia A I ^a   Moumen, A ^a   Carro, M D ^b  

^a CSIC   (Spain)

^b UNIVERSITY OF LEÓN   (Spain)

Author keywords

Amino acids flow; Goats; Olive leaves; Ruminal fermentation; Sheep; Single flow continuous culture fermenters

Indexed keywords

AMINO ACID; NITROGEN;

ANIMAL; ARTICLE; BIOREACTOR; CHEMISTRY; FERMENTATION; GOAT; MALE; METABOLISM; MICROBIOLOGY; OLIVE TREE; PLANT LEAF; RUMINANT STOMACH; SHEEP;

AMINO ACIDS; ANIMALS; BIOREACTORS; FERMENTATION; GOATS; MALE; NITROGEN; OLEA; PLANT LEAVES; RUMEN; SHEEP;

BACTERIA (MICROORGANISMS); CAPRA HIRCUS; HORDEUM; MEDICAGO SATIVA; OVIS ARIES; VICIA FABA;

EID: 77951971948     PISSN: 09312439     EISSN: 14390396     Source Type: Journal    
DOI: 10.1111/j.1439-0396.2008.00905.x     Document Type: Article

References (41)

1. Bach A, Calsamiglia S, Stern M D. Nitrogen metabolism in the rumen. Journal of Dairy Science 2005, 88(E. Suppl.):E9-E21.
2. Balcells J, Guada J A, Peiró J M, Parker D S. Simultaneous determination of allantoin and oxypurines in biological fluids by high-performance liquid chromatography. Journal of Chromatography 1992, 575:153-157.
3. Boguhn J, Kluth H, Rodehutscord M. Effect of total mixed ration composition on amino acid profiles of different fractions of ruminal microbes. Journal of Dairy Science 2006, 89:1592-1603.
4. Calsamiglia S, Stern M D, Firkins J L. Effects of protein source on nitrogen metabolism in continuous culture and intestinal digestion in vitro. Journal of Animal Science 1995, 73:1819-1827.
5. Carro M D, Ranilla M J, Martín-García A I, Molina-Alcaide E. Comparison of microbial fermentation of high- and low-forage diets in sheep rumen, Rusitec and single-flow continuous culture fermenters. Animal 2008
6. Clark J H, Klusmeyer T H, Cameron M R. Microbial protein synthesis and flows of nitrogen fractions to the duodenum of dairy cows. Journal of Dairy Science 1992, 75:2304-2323.
7. Czerkawski J W. Chemical composition of microbial matter in the rumen. Journal of the Science of Food and Agriculture 1976, 27:621-632.
8. Dewhurst R J, Davies D R, Ferry R J. Microbial protein supply from the rumen. Animal Feed Science and Technology 2000, 85:1-21.
9. Fegeros K, Zervas G, Apsokardos F, Vastardis J, Apostolaki E. Nutritive evaluation of ammonia treated olive tree leaves for lactating sheep. Small Ruminant Research 1995, 17:9-15.
10. Fernández-Fígares I, Prieto C, Nieto R, Aguilera J F. Free amino acid concentrations in plasma, muscle and liver as indirect measures of protein adequacy in growing chickens. Animal Science 1997, 64:529-539.
11. Hume I D. Synthesis of microbial protein in the rumen. II. A response to higher volatile fatty acids. Australian Journal of Agricultural Research 1970, 21:297-304.
12. Huston J E, Rector B S, Ellis W C, Allen M L. Synamics of digestion in cattle, sheep, goats and deer. Journal of Animal Science 1986, 62:208-215.
13. World olive oil figures: production. 2008, http://www.internationaloliveoil.org, Available at Accessed 30 July 2008
14. Isac M D, García M A, Aguilera J F, Molina-Alcaide E. A comparative study of nutrient digestibility, kinetics of digestion and passage and rumen fermentation pattern in goats and sheep offered medium quality forages at the maintenance level of feeding. Archives of Animal Nutrition 1994, 46:37-50.
15. Korhonen M, Ahvenjärvi S, Vanhatalo A, Huhtanen P. Supplementing barley or rapeseed meal to dairy cows fed grass-red clover silage. II. Amino acid profile of microbial fractions. Journal of Animal Science 2002, 80:2188-2196.
16. Makkar H P S. Effects and fate of tannins in ruminant animals, adaptation to tannins, and strategies to overcome detrimental effects of feeding tannin-rich feeds. Small Ruminant Research 2003, 49:241-256.
17. Martin C, Bernard L, Michalet-Doreau B. Influence of sampling time and diet on amino acid composition of protozoal and bacterial fractions from bovine ruminal contents. Journal of Animal Science 1996, 74:1157-1163.
18. Martín-García A I, Yáñez-Ruiz D R, Moumen A, Molina-Alcaide E. Effect of polyethylene glycol, urea and sunflower meal on olive (Olea europaea var. europaea) leaf fermentation in continuous fermenters. Small Ruminant Research 2006, 61:53-61.
19. McDougall E I. Studies on ruminant saliva. I. The composition and output of sheep's saliva. The Biochemical Journal 1948, 43:99-109.
20. Mehrez A L, Ørskov E R, McDonald I. Rates of rumen fermentation in relation to rumen ammonia concentration. The British Journal of Nutrition 1977, 38:437-443.
21. Michalet-Doreau B, Ould-Bah M. In vitro and in sacco methods for the estimation of dietary nitrogen degradability in the rumen. Animal Feed Science and Technology 1992, 40:57-86.
22. Miettinen H, Setälä J. Design and development of a continuous culture system to study rumen fermentation. Journal of Agricultural Science 1989, 61:463-473.
23. Molina-Alcaide E, Weisbjerg M R, Hvelplund T. Degradation characteristics of shrubs and the effect of supplementation with urea or protein on microbial production using a continuous-culture system. Journal of Animal Physiology and Animal Nutrition 1996, 75:121-132.
24. Molina-Alcaide E, Martín-García I, Aguilera J F. A comparative study of nutrient digestibility, kinetics of degradation and passage and rumen fermentation pattern in goats and sheep offered good quality diets. Livestock Production Science 2000, 64:215-223.
25. Molina-Alcaide E, Yáñez-Ruiz D R, Moumen A, Martín-García I. Chemical composition and nitrogen availability for goats and sheep of some olive by-products. Small Ruminant Research 2003a, 49:329-336.
26. Molina-Alcaide E, Yáñez-Ruiz D R, Moumen A, Martín-García I. Ruminal degradability and in vitro intestinal digestibility of sunflower meal and in vitro digestibility of olive by-products supplemented with urea or sunflower meal. Comparison between goats and sheep. Animal Feed Science and Technology 2003b, 110:3-15.
27. Molina-Alcaide E, Moumen A, Martín-García I, Carro M D. Comparison of bacterial pellets and microbial markers for the estimation of the microbial nitrogen and amino acids flows from single flow continuous culture fermenters fed diets containing two-stage olive cake. Journal of Animal Physiology and Animal Nutrition 2008, 10.1111/j.1439-0396.2008.00834.x, DOI
28. Moumen A, Yáñez-Ruiz D R, Martín-García I, Molina-Alcaide E. Fermentation characteristics and microbial growth promoted by diets including two-phase olive cake in continuous fermenters. Journal of Animal Physiology and Animal Nutrition 2008, 92:9-17.
29. Nefzaoui A. Valorisation des residus lignocellulosiques dans la′limentation des ruminants par les traitments aux a′lcalis. Application aux grignon d'olive. 1985, PhD Dissertation, Universite′ Catholique de Louvain, Louvain-la-Neuve, Belgium
30. Ørskov E R. Protein Nutrition in Ruminants 1988, Academic Press, London, UK
31. de Rancourt M, Fois N, Lavín M, Tchakérian E, Vallerand F. Mediterranean sheep and goats production: an uncertain future. Small Ruminant Research 2006, 62:167-179.
32. Ranilla M J, López S, Giráldez F J, Valdés C, Carro M D. Comparative digestibility and digesta flow kinetics in two breeds of sheep. Animal Science 1998, 66:389-396.
33. Rodríguez-Prado M, Calsamiglia S, Ferret A. Effects of fiber content and particles size of forage on the flow of microbial amino acids from continuous culture fermenters. Journal of Dairy Science 2004, 87:1413-1424.
34. SAS/STAT User's Guide: Statistics (Release 8.02) 2002, SAS Institute, Cary, NC, USA
35. Silanikove N. The physiological basis of adaptation in goats to harsh environments. Small Ruminant Research 2000, 35:181-193.
36. Slyter L L, Putnam P A. In vivo vs. in vitro continuous culture of ruminal microbial populations. Journal of Animal Science 1967, 26:1421-1427.
37. van Soest P J. Nutritional Ecology of the Ruminant 1994, 2nd edn, Cornell University Press, Ithaca, NY, USA
38. Wallace R J, Onodera R, Cotta M A. Metabolism of nitrogen-containing compounds. The Rumen Microbial Ecosystem 1997, 283-328. Hobson P N, Stewart C S, In, ed, Chapman and Hall, London, UK, pp
39. Weatherburn M W. Phenol-hypochlorite reaction for determination of ammonia. Analytical Chemistry 1967, 39:971-974.
40. Yáñez-Ruiz D R, Martín-García A I, Moumen A, Molina-Alcaide E. Ruminal fermentation and degradation patterns, protozoa population and urinary purine derivatives excretion in goats and wethers fed diets based on olive leaves. Journal of Animal Science 2004a, 82:3006-3014.
41. Yáñez-Ruiz D R, Moumen A, Martín-García A I, Molina-Alcaide E. Ruminal fermentation and degradation patterns, protozoa population, and urinary purine derivates excretion in goats and wethers fed diets based on two-stage olive cake: effect of PEG supply. Journal of Animal Science 2004b, 82:2023-2032.