The feasibility of using lithium-heparin plasma from a gel separator tube as a substitute for serum in clinical biochemical tests

Laboratory Medicine

Volumn 41, Issue 4, 2010, Pages 215-219

  Wei, Yuan Hua ^a   Zhang, Chun Bing ^a   Yang, Xue Wen ^a   Ji, Ming De ^a  

^a THE FIRST AFFILIATED HOSPITAL OF NANJING MEDICAL UNIVERSITY   (China)

Author keywords

Analytes; Gel separator; Plasma; Serum; Specimen types

Indexed keywords

HEPARIN; LITHIUM;

ARTICLE; CENTRIFUGATION; CONTROLLED STUDY; GEL SEPARATOR TUBE; HUMAN; INTERMETHOD COMPARISON; LABORATORY TEST; LITHIUM HEPARIN PLASMA; PLASMA; SEPARATION TECHNIQUE; SERUM; TIME; TUBE;

EID: 77951942988     PISSN: 00075027     EISSN: None     Source Type: Journal    
DOI: 10.1309/LMIXVAI70KS0UWQI     Document Type: Article

References (25)

1. Anderson NR, Chatha K, Holland MR, et al. Effect of sample tube type and time to separation on in vitro levels of C-reactive protein. Br J Biomed Sci. 2003;60:164-165. (Pubitemid 37204578)
2. Banfi G, Bauer K, Brand W, et al. Use of Anticoagulants in Diagnostic Laboratory Investigations. Geneva, Switzerland: World Health Organization; 2002. WHO document WHO/DIL/LAB/99.1 Rev 2.
3. Stokol T, Nydam DV. Effect of anticoagulant and storage conditions on bovine nonesterified fatty acid and beta-hydroxybutyrate concentrations in blood. J Dairy Sci. 2005;88:3139-3144. (Pubitemid 41542447)
4. Ernst DJ, Calam R. NCCLS simplifies the order of draw: A brief history. MLO Med Lab Obs. 2004;36:26-27.
5. Gerlach RF, Demacq C, Jung K, et al. Rapid separation of serum does not avoid artificially higher matrix metalloproteinase (MMP)-9 levels in serum versus plasma. Clin Biochem. 2007;40:119-123. (Pubitemid 46014168)
6. Lum G, Gambino SR. A comparison of serum versus heparinized plasma for routine chemistry tests. Am J Clin Pathol. 1974;61:108-113.
7. Banfi G. State of the art of preanalysis in laboratories in Italy performing endocrinological tests. Eur J Clin Chem Clin Biochem. 1995;33:99-101.
8. Collinson PO, Thomas S, Siu L, et al. Rapid troponin T measurement in whole blood for detection of myocardial damage. Ann Clin Biochem. 1995;32:454-458.
9. Siebers RW, Chen CT, Ferguson RI, et al. Effect of blood sample tubes on amiodarone and desethylamiodarone concentrations. Ther Drug Monit. 1988;10:349-351.
10. Gennaro MC, Calvino R, Abrigo C. Ion interaction reagent reversed-phase high-performance liquid chromatography determination of anti-tuberculosis drugs and metabolites in biological fluids. J Chromatogr B Biomed Sci Appl. 2001;754:477-486. (Pubitemid 32261043)
11. Develter M, Blanckaert N, Komarek A, et al. Can heparin plasma be used instead of serum for nephelometric analysis of serum proteins? Clin Chem. 2006;52:1609-1610. (Pubitemid 44148330)
12. Boyanton BL, Jr, Blick KE. Stability studies of twenty-four analytes in human plasma and serum. Clin Chem. 2002;48:2242-2247.
13. O'Keane MP, Cunningham SK. Evaluation of three different specimen types (serum, plasma lithium heparin and serum gel separator) for analysis of certain analytes: Clinical significance of differences in results and efficiency in use. Clin Chem Lab Med. 2006;44:662-668.
14. Karppi J, Akerman KK, Parviainen M. Suitability of collection tubes with separator gels for collecting and storing blood samples for therapeutic drug monitoring (TDM). Clin Chem Lab Med. 2000;38:313-320. (Pubitemid 30444958)
15. Jones PG, McClelland A, McNeil A, et al. Agreement between troponin T levels from plain and heparinized tubes. Emerg Med (Fremantle). 2001;13:451-455. (Pubitemid 34042157)
16. Wallace AM. Measurement of leptin and leptin binding in the human circulation. Ann Clin Biochem. 2000;37:244-252.
17. Numata Y, Dohi K, Furukawa A, et al. Immunoradiometric assay for the N-terminal fragment of proatrial natriuretic peptide in human plasma. Clin Chem. 1998;44:1008-1013.
18. Calam RR, Mansoor I, Blaga J. Homocysteine stability in heparinized plasma stored in a gel separator tube. Clin Chem. 2005;51:1554-1555.
19. Spiritus T, Zaman Z, Desmet W. Iodinated contrast media interfere with gel barrier formation in plasma and serum separator tubes. Clin Chem. 2003;49:1187-1189.
20. Hegstad-Davies RL. A review of sample handling considerations for reproductive and thyroid hormone measurement in serum or plasma. Theriogenology. 2006;66:592-598.
21. Bowen RA, Vu C, Remaley AT, et al. Differential effect of blood collection tubes on total free fatty acids (FFA) and total triiodothyronine (TT3) concentration: A model for studying interference from tube constituents. Clin Chim Acta. 2007;378:181-193.
22. Hira K, Ohtani Y, Rahman M, et al. Pseudohyperkalaemia caused by recentrifugation of blood samples after storage in gel separator tubes. Ann Clin Biochem. 2001;38:386-390.
23. Doumas BT, Hause LL, Simuncak DM, et al. Differences between values for plasma and serum in tests performed in the Ektachem 700 XR Analyzer, and evaluation of plasma separator tubes (PST). Clin Chem. 1989;35:151-153.
24. Zhang DJ, Elswick RK, Miller WG, et al. Effect of serum-clot contact time on clinical chemistry laboratory results. Clin Chem. 1998;44:1325-1333.
25. Lowbeer C, Wallinder H. Stability of IMMULITE 2000 plasma NT-proBNP in tubes with separator gel stored at room temperature. Clin Chim Acta. 2007;377:283-284.