GST-tagged mouse estrogen receptor a-transactivation domain fusion protein is specifically degraded during its over-expression in E. coli and purification

Molecular Biology Reports

Volumn 37, Issue 3, 2010, Pages 1335-1340

  Thakur, M K ^a   Ghosh, Swati ^a  

^a BANARAS HINDU UNIVERSITY   (India)

Author keywords

Estrogen receptor ; Fusion protein; Induction; Lysate; Overproduction; Protein purification

Indexed keywords

ESTROGEN RECEPTOR ALPHA; GLUTATHIONE TRANSFERASE; HYBRID PROTEIN; PROTEINASE; TRANSACTIVATOR PROTEIN; RECOMBINANT PROTEIN;

AFFINITY CHROMATOGRAPHY; ARTICLE; BACTERIAL GROWTH; CONCENTRATION (PARAMETERS); CONTROLLED STUDY; ENZYME INDUCTION; ESCHERICHIA COLI; GENE EXPRESSION SYSTEM; GENE OVEREXPRESSION; MOLECULAR WEIGHT; NONHUMAN; PROTEIN ANALYSIS; PROTEIN DEGRADATION; PROTEIN DOMAIN; PROTEIN EXPRESSION; PROTEIN PURIFICATION; ANIMAL; CHEMISTRY; GENETICS; IMMUNOBLOTTING; ISOLATION AND PURIFICATION; METABOLISM; MOUSE;

ESCHERICHIA COLI;

ANIMALS; CHROMATOGRAPHY, AFFINITY; ESCHERICHIA COLI; ESTROGEN RECEPTOR ALPHA; IMMUNOBLOTTING; MICE; MOLECULAR WEIGHT; RECOMBINANT PROTEINS; TRANS-ACTIVATORS;

EID: 77951900959     PISSN: 03014851     EISSN: 15734978     Source Type: Journal    
DOI: 10.1007/s11033-009-9512-8     Document Type: Article

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