Using oocytes for Wnt signaling assays: Paracrine assays and Wnt-conditioned medium

Methods

Volumn 51, Issue 1, 2010, Pages 52-55

  Cha, Sang wook ^a   Heasman, Janet ^a  

^a CINCINNATI CHILDREN S HOSPITAL RESEARCH FOUNDATION   (United States)

Author keywords

Conditioned medium; Oocyte; Paracrine assay; Wnt; Xenopus

Indexed keywords

GREEN FLUORESCENT PROTEIN; MESSENGER RNA; RECOMBINANT PROTEIN; WNT PROTEIN; WNT11 PROTEIN; WNT5A PROTEIN; CULTURE MEDIUM; LIGAND;

CELL FUNCTION; CELL ISOLATION; CULTURE MEDIUM; EXPLANT; IMMUNOHISTOCHEMISTRY; MEDIATOR RELEASE; NONHUMAN; OOCYTE; PARACRINE SIGNALING; PRIORITY JOURNAL; PROTEIN LOCALIZATION; REVERSE TRANSCRIPTION POLYMERASE CHAIN REACTION; REVIEW; RNA TRANSLATION; SIGNAL TRANSDUCTION; TRANSCRIPTION REGULATION; XENOPUS; ALGORITHM; ANIMAL; CELL LINE; FEMALE; HUMAN; METABOLISM; MOUSE; XENOPUS LAEVIS;

ALGORITHMS; ANIMALS; CELL LINE; CULTURE MEDIA, CONDITIONED; FEMALE; GREEN FLUORESCENT PROTEINS; HUMANS; LIGANDS; MICE; OOCYTES; RECOMBINANT PROTEINS; REVERSE TRANSCRIPTASE POLYMERASE CHAIN REACTION; SIGNAL TRANSDUCTION; WNT PROTEINS; XENOPUS LAEVIS;

ANIMALIA;

EID: 77951879438     PISSN: 10462023     EISSN: 10959130     Source Type: Journal    
DOI: 10.1016/j.ymeth.2010.01.029     Document Type: Review

References (16)

1. Schier A.F., and Shen M.M. Nature 403 (2000) 385-389
2. Reversade B., Kuroda H., Lee H., Mays A., and De Robertis E.M. Development 132 (2005) 3381-3392
3. Reissmann E., Jörnvall H., Blokzijl A., Andersson O., Chang C., Minchiotti G., Persico M.G., Ibáñez C.F., and Brivanlou A.H. Genes Dev. 15 (2001) 2010-2022
4. Cha S., Tadjuidje E., White J., Wells J., Mayhew C., Wylie C., and Heasman J. Curr. Biol. 19 (2009) 1573-1580
5. Schwarz-Romond T., Metcalfe C., and Bienz M. J. Cell Sci. 120 (2007) 2402-2412
6. Yamanaka H., and Nishida E. Genes Cells 12 (2007) 961-967
7. Borchert K.M., Galvin R.J., Frolik C.A., Hale L.V., Halladay D.L., Gonyier R.J., Trask O.J., Nickischer D.R., and Houck K.A. Assay Drug Dev. Technol. 3 (2005) 133-141
8. Bilic J., Huang Y.L., Davidson G., Zimmermann T., Cruciat C.M., Bienz M., and Niehrs C. Science 316 (2007) 1619-1622
9. Lustig K.D., and Kirschner M.W. Proc. Natl. Acad. Sci. USA 92 (1995) 6234-6238
10. Birsoy B., Berg L., Williams P.H., Smith J.C., Wylie C.C., Christian J.L., and Heasman J. Development 132 (2005) 591-602
11. Hausen P., and Riebesell M. The Early Development of Xenopus Laevis: An Atlas of the Histology (2001), Springer-Verlag Telo
12. Wardle F.C., Welch J.V., and Dale L. Mech. Dev. 86 (1999) 75-85
13. Ryan K., Garrett N., Bourillot P., Stennard F., and Gurdon J.B. Mech. Dev. 94 (2000) 133-146
14. Sive H.L., Grainger R.M., and Harland R.M. Early Development of Xenopus laevis: A Laboratory Manual (2000), Cold Spring Harbor Laboratory Press
15. Lee C., Kieserman E., Gray R.S., Park T., and Wallingford J. CSH protocols (2008) 46-49
16. Lavery D.L., and Hoppler S. Methods Mol. Biol. 469 (2008) 401-415