1H, 13C, and 15N NMR assignments of StnII-Y111N, a highly impaired mutant of the sea anemone actinoporin Sticholysin II

Biomolecular NMR Assignments

Volumn 4, Issue 1, 2010, Pages 69-72

  Pardo Cea, Miguel A ^a   Alegre Cebollada, Jorge ^b,c   Martínez Del Pozo, Álvaro ^b   Gavilanes, José G ^b   Bruix, Marta ^a  

^a INSTITUTO DE QUÍMICA FÍSICA ROCASOLANO   (Spain)

^b UNIVERSIDAD COMPLUTENSE DE MADRID   (Spain)

^c Och Spine at New York Presbyterian Hospitals   (United States)

Author keywords

Actinoporin; Equinatoxin; NMR resonance assignment; Sticholysin

Indexed keywords

ACTINIARIA; HELIANTHUS; STICHODACTYLA HELIANTHUS;

CARBON; COELENTERATE VENOM; HYDROGEN; MUTANT PROTEIN; NITROGEN; STICHOLYSIN II;

AMINO ACID SEQUENCE; ANIMAL; ARTICLE; CHEMISTRY; ESCHERICHIA COLI; GENETIC VARIABILITY; GENETICS; MISSENSE MUTATION; NUCLEAR MAGNETIC RESONANCE; PH; SEA ANEMONE; TEMPERATURE;

AMINO ACID SEQUENCE; ANIMALS; CARBON ISOTOPES; CNIDARIAN VENOMS; ESCHERICHIA COLI; GENETIC VARIATION; HYDROGEN; HYDROGEN-ION CONCENTRATION; MUTANT PROTEINS; MUTATION, MISSENSE; NITROGEN ISOTOPES; NUCLEAR MAGNETIC RESONANCE, BIOMOLECULAR; SEA ANEMONES; TEMPERATURE;

EID: 77951877935     PISSN: 18742718     EISSN: 1874270X     Source Type: Journal    
DOI: 10.1007/s12104-010-9214-0     Document Type: Article

References (12)

1. J Alegre-Cebollada M Oñaderra JG Gavilanes A Martínez-del- Pozo 2007 Sea anemone actinoporins: the transition from a folded soluble state to a functionally active membrane-bound oligomeric pore Curr Protein Pept Sci 8 558 572 10.2174/138920307783018686 (Pubitemid 351266989)
2. J Alegre-Cebollada G Clementi M Cunietti C Porres M Oñaderra JG Gavilanes A Martínez-del-Pozo 2007 Silent mutations at the 5 '-end of the cDNA of actinoporins from the sea anemone Stichodactyla helianthus allow their heterologous overproduction in Escherichia coli J Biotechnol 127 211 221 10.1016/j.jbiotec.2006.07.006 (Pubitemid 46107142)
3. J Alegre-Cebollada M Cunietti E Herrero-Galán JG Gavilanes A Martínez-del-Pozo 2008 Calorimetric scrutiny of lipid binding by sticholysin II toxin mutants J Mol Biol 382 920 930 10.1016/j.jmb.2008.07.053
4. G Anderluh P Macek 2002 Cytolytic peptide and protein toxins from sea anemones (Anthozoa: Actiniaria) Toxicon 40 111 124 10.1016/S0041-0101(01)00191-X (Pubitemid 33016540)
5. A Athanasiadis G Anderluh P Macek D Turk 2001 Crystal structure of the soluble form of equinatoxin II, a pore-forming toxin from the sea anemone Actinia equina Structure 9 341 346 10.1016/S0969-2126(01)00592-5 (Pubitemid 32772579)
6. C Bartels P Güntert M Billeter K Wüthrich 1997 GARANT-a general algorithm for resonance assignment of multidimensional nuclear magnetic resonance spectra J Comput Chem 18 139 149 10.1002/(SICI)1096-987X(19970115)18: 1<139::AID-JCC13>3.0.CO;2-H (Pubitemid 127599839)
7. 15N NMR assignments of StnII-R29Q, a defective lipid binding mutant of the sea anemone actinoporin Sticholysin II Biomol NMR Assign 3 239 241 10.1007/s12104-009-9184-2
8. 15N NMR assignments of the actinoporin Sticholysin I Biomol NMR Assign 3 5 7 10.1007/s12104-008-9127-3
9. Goddard TD, Kneller DG (2005) SPARKY 3. University of California, San Francisco
10. MG Hinds W Zhang G Anderluh PE Hansen RS Norton 2002 Solution structure of the eukaryotic pore-forming cytolysin equinatoxin II: implications for pore formation J Mol Biol 315 1219 1229 10.1006/jmbi.2001.5321 (Pubitemid 34729299)
11. KC Kristan G Viero M Dalla Serra P Macek G Anderluh 2009 Molecular mechanism of pore formation by actinoporins Toxicon 54 1125 1134 10.1016/j.toxicon.2009.02.026
12. JM Mancheño J Martín-Benito M Martínez-Ripoll JG Gavilanes JA Hermoso 2003 Crystal and electron microscopy structures of sticholysin II actinoporin reveal insights into the mechanism of membrane pore formation Structure 11 1319 1328 10.1016/j.str.2003.09.019 (Pubitemid 37412414)