Silent mutations at the 5′-end of the cDNA of actinoporins from the sea anemone Stichodactyla helianthus allow their heterologous overproduction in Escherichia coli

Journal of Biotechnology

Volumn 127, Issue 2, 2007, Pages 211-221

  Alegre Cebollada, Jorge ^a   Clementi, Giorgia ^a   Cunietti, Michela ^a   Porres, Christian ^a   Oñaderra, Mercedes ^a   Gavilanes, José G ^a   Pozo, Álvaro Martínez del ^a  

^a UNIVERSIDAD COMPLUTENSE DE MADRID   (Spain)

Author keywords

Actinoporin; Equinatoxin; Protein production; Ribosome binding site; Silent mutation; Sticholysin

Indexed keywords

DNA; ESCHERICHIA COLI; MUTAGENESIS; RNA; SPECTROSCOPIC ANALYSIS;

ACTINOPORIN; CDNA; EQUINATOXIN; LYTIC ACTIVITY; PROTEIN PRODUCTION; RIBOSOME BINDING SITE; SEA ANEMONE; STICHODACTYLA HELIANTHUS; STICHOLYSIN;

PROTEINS;

ACTINOPORIN; COMPLEMENTARY DNA; MUTANT PROTEIN; PROTEIN; RECOMBINANT PROTEIN; RNA; UNCLASSIFIED DRUG;

ARTICLE; BINDING SITE; CODON; CONTROLLED STUDY; DNA FLANKING REGION; DNA SEQUENCE; ENZYME ACTIVITY; ESCHERICHIA COLI; GENE EXPRESSION SYSTEM; GENETIC RECOMBINATION; HETEROLOGOUS EXPRESSION; MUTAGENESIS; NONHUMAN; PRIORITY JOURNAL; PROTEIN SYNTHESIS; PROTEIN VARIANT; RIBOSOME; RNA STRUCTURE; SEA ANEMONE; SPECTROSCOPY; STICHODACTYLA HELIANTHUS;

ANIMALS; BASE SEQUENCE; CIRCULAR DICHROISM; DNA, COMPLEMENTARY; ELECTROPHORESIS, POLYACRYLAMIDE GEL; ESCHERICHIA COLI; MOLECULAR SEQUENCE DATA; MUTANT PROTEINS; MUTATION; NUCLEIC ACID CONFORMATION; PLASMIDS; PROTEIN DENATURATION; PROTEIN ISOFORMS; RECOMBINANT PROTEINS; SEA ANEMONES;

ACTINIARIA; ESCHERICHIA COLI; STICHODACTYLA HELIANTHUS;

EID: 33846197988     PISSN: 01681656     EISSN: None     Source Type: Journal    
DOI: 10.1016/j.jbiotec.2006.07.006     Document Type: Article

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