Click-iT proliferation assay with improved DNA histograms

Current Protocols in Cytometry

Volumn , Issue SUPPL. 52, 2010, Pages

  Krishan, Awtar ^a   Hamelik, Ronald M ^a  

^a UNIVERSITY OF MIAMI MILLER SCHOOL OF MEDICINE   (United States)

Author keywords

Cell cycle; Click iT assay; DNA content; Flow cytometry; Proliferation assay

Indexed keywords

5 ETHYNYLDEOXYURIDINE; 7 AMINODACTINOMYCIN; PARAFORMALDEHYDE; PROPIDIUM IODIDE; THYMIDINE DERIVATIVE; UNCLASSIFIED DRUG;

CELL ASSAY; CELL DIVISION; CELL LABELING; CELL PERMEABILIZATION; CELL PROLIFERATION; CONTROLLED STUDY; CYTOLYSIS; DNA CONTENT; DNA DETERMINATION; DNA SYNTHESIS; FLOW CYTOMETRY; HISTOGRAM; INTERMETHOD COMPARISON; PRIORITY JOURNAL; REVIEW;

ANIMALS; CELL NUCLEUS; CELL PROLIFERATION; DACTINOMYCIN; DNA; FLOW CYTOMETRY; HUMANS; HYDRAZINES; PROPIDIUM;

ALEXA;

EID: 77951848734     PISSN: 19349297     EISSN: 19349300     Source Type: Journal    
DOI: 10.1002/0471142956.cy0736s52     Document Type: Review

References (15)

1. Cappella, P., Gasparri, F., Pulici, M., and, Moll, J., 2008. A novel method based on click chemistry, which overcomes limitations of cell cycle analysis by classical determination of BrdU incorporation, allowing multiplex antibody staining. Cytom. Part A 73: 626 - 636.
2. Darzynkiewicz, Z., Traganos, F., Kapuscinski, J., Staiano-Coico, L., and, Melamed, M.R., 1984. Accessibility of DNA in situ to various fluorochromes: Relationship to chromatin changes during erythroid differentiation of Friend leukemia cells. Cytom. Part A 5: 355 - 363.
3. Diermeier-Daucher, S., Clarke, S.T., Hill, D., Vollmann-Zwerenz, A., Bradford, J.A., and, Brockhoff, G., 2009. Cell type specific applicability of 5-ethynyl-2-deoxyuridine (EdU) for dynamic proliferation assessment in flow cytometry. Cytom. Part A 75: 535 - 546.
4. Dolbeare, F., Kuo, W.L., Beisker, W., Vanderlaan, M., and, Gray, J.W., 1990. Using monoclonal antibodies in bromodeoxyuridine-DNA analysis. Methods Cell Biol. 33: 207 - 216.
5. Gratzner, H.G., 1882. Monoclonal antibody to 5-bromo- and 5-iododeoxyuridine: A new reagent for detection of DNA replication. Science 218: 474 - 475.
6. Hamelik, R.M., and, Krishan, A., 2009. Click-iT assay with improved DNA distribution histograms. Cytom. Part A 75: 862 - 865.
7. Krishan, A., 1990. Rapid DNA content analysis by the propidium iodide-hypotonic citrate method. Methods Cell Biol. 33: 121 - 125.
8. Leif, R.C., Stein, J.H., and, Zucker, R.M., 2004. A short history of the initial application of anti-5-BrdU to the detection and measurement of S phase. Cytom. Part A 58: 45 - 52.
9. Li, X., Melamed, M.R., and, Darzynkiewicz, Z., 1996. Detection of apoptosis and DNA replication by differential labeling of DNA strand breaks with fluorochromes of different color. Exp. Cell Res. 222: 28 - 37.
10. Moran, R., Darzynkiewicz, Z., Staiano-Coico, L., and, Melamed, M.R., 1985. Detection of BrdUrd incorporation by monoclonal antibodies: Role of the DNA denaturation step. J. Histochem. Cytochem. 33: 821 - 827.
11. Rousselle, C., Robert-Nicoud, M., and, Ronot, X., 1998. Flow cytometric analysis of DNA content of living and fixed cells: A comparative study using various fixatives. Histochem. J. 30: 773 - 781.
12. Salic, A., and, Mitchison, T.J., 2008. A chemical method for fast and sensitive detection of DNA synthesis in vivo. Proc. Natl. Acad. Sci. U.S.A. 105: 2415 - 2420.
13. Schimenti,.K.J., and, Jacobberger, J.W., 1992. Fixation of mammalian cells for flow cytometric evaluation of DNA content and nuclear immunofluorescence. Cytom. Part A 13: 48 - 59.
14. Terry, N.H., and, White, R.A., 2001. Cell cycle kinetics estimated by analysis of bromodeoxyuridine incorporation. Methods Cell Biol. 63: 355 - 374.
15. Toba, K., Winton, E.F., and, Bray, R.A., 1992. Improved staining method for the simultaneous flow cytofluorometric analysis of DNA content, S-phase fraction, and surface phenotype using single laser instrumentation. Cytom. Part A 13: 60 - 67.