Detecting potential pathogens on hospital surfaces: An assessment of carpet tile flooring in the hospital patient environment

Indoor and Built Environment

Volumn 19, Issue 2, 2010, Pages 239-249

  Harris, Debra D ^a   Pacheco, Adriana ^b   Lindner, Angela S ^a  

^a UNIVERSITY OF FLORIDA   (United States)

^b J Crayton Pruitt Family Department of Biomedical Engineering   (United States)

Author keywords

Contamination; Disinfection; Environmental surfaces; Flooring; Hospital; Infection control

Indexed keywords

DNA 16S;

ARTICLE; BACILLUS; BACTERIUM CONTAMINATION; BACTERIUM DETECTION; BACTERIUM IDENTIFICATION; COMMUNITY HOSPITAL; CONTROLLED STUDY; DENATURING GRADIENT GEL ELECTROPHORESIS; ENVIRONMENTAL MONITORING; HOSPITAL BUILDING; HOSPITAL INFECTION; INFECTION CONTROL; MICROBIAL COMMUNITY; MICROBIAL DIVERSITY; NUCLEOTIDE SEQUENCE; PRIORITY JOURNAL; PSYCHROBACTER; UNINDEXED SEQUENCE; UNITED STATES;

EID: 77951735928     PISSN: 1420326X     EISSN: 14230070     Source Type: Journal    
DOI: 10.1177/1420326X09347050     Document Type: Article

References (35)

1. Garner JS, Jarvis WR, Emori TG, Horan TC, Hughes JM: CDC definitions for nosocomial infections: in Olmstead RN (ed.): APIC Infection Control and Applied Epidemiology: Principles and Practice, St. Louis, CDC, 1996, pp. A1 - A20.
2. Boyce JM: Environmental contamination makes an important contribution to hospital infection: J Hosp Infect 2007 ; 65 (Suppl 2). 50-54.
3. Adams-Chapman I., Stoll B.: Prevention of nosocomial infections in the neonatal intensive care unit: Curr Opin Pediatr 2002 ; 14: 157-164. (Pubitemid 34412158)
4. Cohen B., Saiman L., Cimiotti J., Larson E.: Factors associated with hand hygiene practices in two neonatal intensive care units: Pediatr Infect Dis J 2003 ; 22: 494-499. (Pubitemid 36782187)
5. Gelber S., Ratner A.: Hospital-acquired viral pathogens in the neonatal intensive care unit: Semin Perinatol 2002 ; 26: 346-356. (Pubitemid 35291794)
6. Saiman L.: Risk factors for hospital-acquired infections in the neonatal intensive care unit: Semin Perinatol 2002 ; 26: 315-321. (Pubitemid 35291790)
7. Oren I., Haddad N., Finkelstein R., Rowe JM: Invasive pulmonary aspergillosis in neutropenic patients during hospital construction: before and after chemoprophylaxis and institution of HEPA filters: Am J Hematol 2001 ; 66: 257-262. (Pubitemid 32210293)
8. Dharan S., Pittet D.: Environmental controls in operating theatres: J Hosp Infect 2002 ; 51: 79-84. (Pubitemid 37554816)
9. Klevens RM, Edwards JR, Richards CL, Horan TC, Gaynes RP, Pollock DA, Cardo DM: estimating health care-associated infections and deaths in U.S. hospitals, 2002: Publ Health Rep 2007 ; 122: 160-166.
10. Hota B.: Contamination, disinfection, and cross-colonization: are hospital surfaces reservoirs for nosocomial infection? Clin Infect Dis 2004 ; 39: 1182-1189. (Pubitemid 40169318)
11. Dancer SJ: Mopping up hospital infection: J Hosp Infect 1999 ; 43: 85-100.
12. McFarland LV: What's lurking under the bed? Persistance and predominance of particular Clostridium difficile strains in a hospital and the potential role of environmental contamination: Infect Contr Hosp Epidemiol 2002 ; 23: 639-640.
13. Skoutelis AT, Westenfelder GO, Beckerdite M., Phair JP: Hospital carpeting and epidemiology of Clostridium difficile: Am J Infect Contr 1994 ; 22: 212-217.
14. Mekonen ET, Gerding DN, Sambol SP, Pottinger JM, Pulvirenti JJ, Marsh D., Kocka FE, Johnson S.: Predominance of a single restriction endonuclease analysis group with intrahospital subgroup diversity among Clostridium difficile isolates at two Chicago hospitals: Infect Contr Hosp Epidemiol 2002 ; 23: 648-652.
15. Pulvirenti JJ, Gerding DN, Nathan C., Hafiz I., Mehra T., Marsh D., Kocka F., Rice T., Fischer SA, Sergreti J., Weinstein RA: Difference in the incidence of Clostridium difficile among patients infected with human immunodeficiency virus admitted to a public hospital and a private hospital: Infect Contr Hosp Epidemiol 2002 ; 23: 641-647.
16. Anderson RL, Mackel DC, Stoler BS, Mallison GF: Carpeting in hospitals: an epidemiological evaluation: J Clin Microbiol 1982 ; 15: 408-415.
17. Boyce JM, Potter-Bynoe G., Chenevert C., King T.: Environmental contamination due to methicillin-resistant Staphylococcus aureus: possible infection control implications: Infect Contr Hosp Epidemiol 1997 ; 18: 622-627.
18. Martínez JA, Ruthazer R., Hansjosten K., Barefoot L., Snydman DR: Role of environmental contamination as a risk factor for acquisition of vancomycin-resistant enterococci in patients treated in a medical intensive care unit: Arch Intern Med 2003 ; 163: 1905-1912.
19. Peterson LR, Noskin GA: New technology for detecting multidrug-resistant pathogens in the clinical microbiology laboratory: Emerg Infect Dis 2001 ; 7: 306-311.
20. Muyzer G., Dewaal EC, Uitterlinden AG: Profiling of complex microbial populations by denaturing gradient gel electrophoresis analysis of polymerase chain reaction amplified genes coding for 16S ribosomal-RNA: Appl Environ Microbiol 1993 ; 59: 695-700.
21. Khan NN, Wilson BL: An environmental assessment of mold contaminations and potential mycotoxin exposures in the greater Southeast Texas area: J Environ Sci Health A Tox Hazard Subst Environ Eng 2003 ; A38: 2759-2772.
22. Buttner MP, Cruz-Perez P., Stetzenbach LD: Enhanced detection of surface-associated bacteria in indoor environments by quantitative PCR: Appl Environ Microbiol 2001 ; 67: 2564-2570.
23. Henckel T., Friedrich M., Conrad R.: Molecular analyses of the methane-oxidizing microbial community in rice field soil by targeting the genes of the 16S rRNA, particulate methane mono-oxygenase, and methanol dehydrogenase: Appl Environ Microbiol 1999 ; 65: 1980-1990.
24. Chory J., Pollard JD: Separation of small DNA fragments by conventional gel electrophoresis: in Ausubel FM, Brent R, Kingston RE, Moore DD, Seidman JG, Smith JA, Struhl K (eds): Current Protocols in Molecular Biology, Ch. 2: Unit 2.7, New York, John Wiley & Sons, 2001.
25. Altschul SF, Gish W., Miller EW, Lipman DJ: Basic local alignment search tool: J Mol Biol 1990 ; 215: 403-410.
26. Thompson JD, Gibson TJ, Plewniak F., Jeanmougin F., Higgins DG: The CLUSTAL-X windows interface: flexible strategies for multiple sequence alignment aided by quality analysis tools: Nucleic Acids Res 1997 ; 25: 4876-4882.
27. Page RDM: TREEVIEW: an application to display phylogenetic trees on personal computers: Comput Appl Biosci 1996 ; 12: 357-358.
28. Drancourt M., Bollet C., Carlioz A., Martelin R., Gayral J-P., Raoult D.: 16S ribosomal DNA sequence analysis of a large collection of environmental and clinical unidentifiable bacterial isolates: J Clin Microbiol 2000 ; 38: 3623-3630.
29. Lloyd-Puryear M., Wallace D., Baldwin T., Hollis DG: Meningitis caused by Psychrobacter immobilis in an infant: J Clin Microbiol 1991 ; 29: 2041-2042.
30. Bowman J.: The genus Psychrobacter: in Dworkin M (ed.): The Prokaryotes: An Evolving Electronic Resource for the Microbiological Community, 3 rd edn, Vol. 6, New York, Springer-Verlag, 2006, pp. 920-930.
31. Bozal N., Montes MJ, Tudela E., Guinea J.: Characterization of several Psychrobacter strains isolated from Antarctic environments and description of Psychrobacter luti sp. nov. and Psychrobacter fozii sp. Nov: Int J Syst Evol Microbiol 2003 ; 53: 1093-1100.
32. Farrar WE, Reboli AC: The genus Bacillus: medical: in Dworkin M (ed.): The Prokaryotes: An Evolving Electronic Resource for the Microbiological Community, 3 rd edn, Vol. 4, New York, Springer-Verlag, 2006, pp. 609-630.
33. Goodfellow M., Maldonado LA: The families Dietziaceae, Gordoniaceae, Nocardiaceae and Tsukamurellaceae: in Dworkin M (ed.): The Prokaryotes: An Evolving Electronic Resource for the Microbiological Community, 3 rd edn, Vol. 3, New York, Springer-Verlag, 2006, pp. 843-888.
34. Mitchell J.: Impact on carpet tile in a hospital patient unit corridor: an observational case study: Master Thesis, University of Florida, Gainesville, 2006. Available at: http://etd.fcla.edu/UF/UFE0014763/mitchell-j.pdf
35. Sehulster L., Chinn RYW: Guideline for environmental infection control in health-care facilities: recommendations of CDC and the Healthcare Infection Control Practices Advisory Committee (HICPAC). Centers for Disease Control and Prevention, MMWR 52 (No. RR-10 Pt II), 2003. Available at: http://www.cdc.gov/ ncidod/dhqp/gl-environinfection.html ( 8 / 09 )