Following enzyme activity with infrared spectroscopy

Sensors

Volumn 10, Issue 4, 2010, Pages 2626-2637

  Kumar, Saroj ^a   Barth, Andreas ^a  

^a STOCKHOLM UNIVERSITY   (Sweden)

Author keywords

ATR; Enzyme activity; FTIR; Infrared spectroscopy; Vibrational spectroscopy

Indexed keywords

ACTIVITY ASSAYS; ALCOHOL DEHYDROGENASE; ATR; ENZYMATIC ACTIVITIES; ENZYMATIC REACTION; FTIR; INFRARED METHODS; INFRARED SPECTRUM;

FOURIER TRANSFORM INFRARED SPECTROSCOPY; INFRARED SPECTROSCOPY; VIBRATIONAL SPECTROSCOPY;

ENZYME ACTIVITY;

ALCOHOL DEHYDROGENASE; FUMARATE HYDRATASE; PYRUVATE KINASE;

ARTICLE; BIOASSAY; ENZYME ACTIVATION; INFRARED SPECTROSCOPY; METABOLISM; METHODOLOGY;

ALCOHOL DEHYDROGENASE; BIOLOGICAL ASSAY; ENZYME ACTIVATION; FUMARATE HYDRATASE; PYRUVATE KINASE; SPECTROSCOPY, FOURIER TRANSFORM INFRARED;

EID: 77951723990     PISSN: 14248220     EISSN: None     Source Type: Journal    
DOI: 10.3390/s100402626     Document Type: Article

References (32)

1. Jencks, W.P. Infrared measurements in aqueous media. Methods Enzymol. 1963, 6, 914-928.
2. Karmali, K.; Karmali, A.; Teixeira, A.; Curto, M.J.M. The use of Fourier transform infrared spectroscopy to assay for urease from Pseudomonas aeruginosa and Canavalia ensiformis. Anal. Biochem. 2004, 331, 115-121.
3. Fisher, J.; Belasco, J.G.; Khosla, S.; Knowles, J.R. ß-Lactamase proceeds via an acyl-enzyme intermediate. Interaction of the Escherichia coli RTEM enzyme with cefoxitin. Biochemistry 1980, 19, 2895-2901.
4. White, A.J.; Drabble, K.; Ward, S.; Wharton, C.W. Analysis and elimination of protein perturbation in infrared difference spectra of acyl-chymotrypsin ester carbonyl groups by using 13C isotopic substitution. Biochem. J. 1992, 287, 317-323.
5. 2+-ATPase. Biochim. Biophys. Acta 1991, 1057, 115-123.
6. 2+ ATPase during catalytic activity. A Fourier transform infrared (FTIR) study using photolysis of caged ATP to trigger the reaction cycle. FEBS Lett. 1990, 277, 147-150.
7. Thoenges, D.; Barth, A. Direct measurement of enzyme activity with infrared spectroscopy. J. Biomol. Screen. 2002, 7, 353-357.
8. López-Sánchez, M.; Ayora-Cañada, M.J.; Molina-Díaz, A.; Siam, M.; Huber, W.; Quintás, G.; Armenta, S.; Lendl, B. Determination of enzyme activity inhibition by FTIR spectroscopy on the example of fructose bisphosphatase. Anal. Bioanal. Chem. 2009, 394, 2137-2144.
9. Lendl, B.; Krieg, P.; Kellner, R. Determination of alkaline phosphatase activity in human sera by mid-FTIR spectroscopy. Fresenius J. Anal. Chem. 1998, 360, 717-720.
10. Karmali, K.; Karmali, A.; Teixeira, A.; Curto, M.J.M. Assay for glucose oxidase from Aspergillus niger and Penicillium amagasakiense by Fourier transform infrared spectroscopy. Anal. Biochem. 2004, 333, 320-327.
11. Cadet, F.; Pin, F.W.; Rouch, C.; Robert, C.; Baret, P. Enzyme kinetics by mid-infrared spectroscopy: ß-fructosidase study by a one-step assay. Biochim. Biophys. Acta 1995, 1246, 142-150.
12. Schindler, R.; Le Thannh, H.; Lendl, B.; Kellner, R. Determination of enzyme kinetics and chemometric evaluation of reaction products by FTIR spectroscopy on the example of ß-fructofuranosidase. Vib. Spectrosc. 1998, 16, 127-135.
13. Schindler, R.; Lendl, B. Simultaneous determination of enzyme activities by FTIR-spectroscopy in an one-step assay. Anal. Chim. Acta 1999, 391, 19-28.
14. Schindler, R.; Lendl, B.; Kellner, R. Determination of amyloglucosidase activity using flow injection analysis with Fourier transform infrared spectrometric detection. Analyst 1997, 122, 531-534.
15. Schindler, R.; Lendl, B.; Kellner, R. Simultaneous determination of α-amylase and amylogucosidase activities using flow injection analysis with Fourier transform infrared spectroscopic detection and partial least-squares data treatment. Anal. Chim. Acta. 1998, 366, 35-43.
16. Krieg, P.; Lendl, B.; Vonach, R.; Kellner, R. Determination of α-amylase activity using Fourier transform infrared spectroscopy. Fresenius J. Anal. Chem. 1996, 356, 504-507.
17. Pacheco, R.; Serralheiro, M.L.M.; Karmali, A.; Haris, P.I. Measuring enzymatic activity of a recombinant amidase using Fourier transform infrared spectroscopy. Anal. Biochem. 2003, 322, 208-214.
18. Pacheco, R.; Karmali, A.; Serralheiro, M.L.M.; Haris, P.I. Application of Fourier transform infrared spectroscopy for monitoring hydrolysis and synthesis reactions catalysed by a recombinant amidase. Anal. Biochem. 2005, 346, 49-58.
19. Gäb, J.; Melzer, M.; Kehe, K.; Richardt, A.; Blum, M.M. Quantification of hydrolysis of toxic organophosphates and organophosphonates by diisopropyl fluorophosphatasse from Loligo vulgaris by in situ Fourier transform infrared spectroscopy. Anal. Biochem. 2009, 385, 187-193.
20. Wright, W.; Vanderkooi, J.M. Use of IR absorption of the carboxyl group of amino acids and their metabolites to determine pKs, to study proteins, and to monitor enzymatic activity. Biospectroscopy 1997, 3, 457-467.
21. Muthusamy, M.; Burrell, M.R.; Thorneley, R.N.F.; Bornemann, S. Real-time monitoring of the oxalate decarboxylase reaction and probing hydron exchange in the product, formate, using Fourier transform infrared spectroscopy. Biochemistry 2006, 45, 10667-10673.
22. Kansiz, M.; Gapes, J.R.; McNaughton, D.; Lendl, B.; Schuster, K.C. Mid-infrared spectroscopy coupled to sequential injection analysis for the online monitoring of the acetone-butanol fermentation process. Anal. Chim. Acta 2001, 438, 175-186.
23. Seeholzer, S.H.; Jaworowski, A.; Rose, I.A. Enolpyruvate: chemical determination as a pyruvate kinase intermediate. Biochemistry 1991, 30, 727-732.
24. Rose, I.A. Stereochemistry of pyruvate kinase, pyruvate carboxylase, and malate enzyme reactions. J. Biol. Chem. 1970, 245, 6052-6056.
25. Ocha, S. The Enzyme; Summer, B., Mybrack, K., Eds.; Academic press, Inc.: New York, NY, USA, 1951; Volume I, part 2, chapter 40.
26. 2+ binding to pyruvate kinase monitored by infrared spectroscopy. Biophys. J. 2010, in press.
27. Rudbeck, M.E.; Kumar, S.; Mroginski, M.A.; Lill, S.O.N.; Blomberg, M.R.A; Barth, A. Infrared spectrum of phosphoenolpyruvate: computational and experimental studies. J. Phys. Chem. A. 2009, 113, 2935-2942.
28. 2+ ATPase: molecular interpretation of infrared difference spectra. Biophys. J. 1998, 75, 538-544.
29. 18O-labeled triphosphates. J. Am. Chem. Soc. 1988, 110, 392-397.
30. Colthup, N.B.; Daly, L.H.; Wiberley, S.E. Introduction to Infrared and Raman Spectroscopy; 2nd Ed.; Academic Press: New York, NY, USA, 1975.
31. Wang, J.H.; Xiao, D.G.; Deng, H.; Callender, R.; Webb, M.R. Vibrational study of phosphate modes in GDP and GTP and their interaction with magnesium in aqueous solution. Biospectroscopy 1998, 4, 219-227.
32. Mikawa, Y; Brasch, J.W.; Jakobsen, R.J. Polarized infrared spectra of single crystal of ethyl alcohol. Spectrochim. Acta 1971, 27, 529-539.