Determination of enzyme activity inhibition by FTIR spectroscopy on the example of fructose bisphosphatase

Analytical and Bioanalytical Chemistry

Volumn 394, Issue 8, 2009, Pages 2137-2144

  Lopez Sanchez M ^a   Ayora Canada M J ^a   Molina Diaz A ^a   Siam, M ^b   Huber, W ^b   Quintas G ^c   Armenta, S ^c   Lendl, B ^c  

^a UNIVERSITY OF JAÉN   (Spain)

^b F HOFFMANN LA ROCHE LTD   (Switzerland)

^c VIENNA UNIVERSITY OF TECHNOLOGY   (Austria)

Author keywords

Enzyme; Fourier transform infrared spectroscopy; Fructose 1,6 bisphosphatase; Inhibition kinetics; Sequential injection analysis

Indexed keywords

BISPHOSPHATES; COMPETITIVE INHIBITION; CONTROLLED FLOW; ENZYMATIC ASSAY; ENZYMATIC REACTION; FOURIER TRANSFORM INFRARED; FTIR SPECTROSCOPY; INHIBITION KINETICS; LABEL-FREE MONITORING; MICHAELIS-MENTEN CONSTANT; MIDINFRARED; MONOPHOSPHATES; SEQUENTIAL INJECTION; SEQUENTIAL INJECTION ANALYSIS; STOPPED FLOW MODE;

CELL MEMBRANES; ENZYME ACTIVITY; ENZYMES; FOURIER TRANSFORM INFRARED SPECTROSCOPY; FOURIER TRANSFORMS; FRUCTOSE; LABELS; SPECTROSCOPIC ANALYSIS; SPECTROSCOPY; SPECTRUM ANALYSIS; SULFUR COMPOUNDS;

ENZYME INHIBITION;

ADENOSINE PHOSPHATE; FRUCTOSE 1,6 BISPHOSPHATE; FRUCTOSE BISPHOSPHATASE; FRUCTOSE BISPHOSPHATE; FRUCTOSE-1,6-DIPHOSPHATE;

ARTICLE; DRUG ANTAGONISM; ENZYMOLOGY; EQUIPMENT DESIGN; EVALUATION; FLOW INJECTION ANALYSIS; HUMAN; INFRARED SPECTROSCOPY; INSTRUMENTATION; ISOLATION AND PURIFICATION; KINETICS; LIVER; METABOLISM; METHODOLOGY;

ADENOSINE MONOPHOSPHATE; EQUIPMENT DESIGN; FLOW INJECTION ANALYSIS; FRUCTOSE-BISPHOSPHATASE; FRUCTOSEDIPHOSPHATES; HUMANS; KINETICS; LIVER; SPECTROSCOPY, FOURIER TRANSFORM INFRARED;

EID: 68549101522     PISSN: 16182642     EISSN: 16182650     Source Type: Journal    
DOI: 10.1007/s00216-009-2904-8     Document Type: Article

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