Hemoglobin variants detected by hemoglobin A1c (HbA1c) analysis and the effects on HbA1c measurements

International Journal of Diabetes in Developing Countries

Volumn 30, Issue 2, 2010, Pages 86-90

  Nasir, Nadzimah Mohd ^a   Thevarajah, M ^a   Yean, Chew Yee ^b  

^a UNIVERSITY OF MALAYA   (Malaysia)

^b UNIVERSITY MALAYA MEDICAL CENTRE   (Malaysia)

Author keywords

HbA1c; Hemoglobin variants; High performance liquid chromatography; Immunoassay

Indexed keywords

EID: 77951718654     PISSN: 09733930     EISSN: 19983832     Source Type: Journal    
DOI: 10.4103/0973-3930.62598     Document Type: Article

References (20)

1. IFCC Standardization of HbA1c. Available from: http://www.ngsp.org/prog/ IFCCstd.pdf [last accessed on 2008 Dec 22]
2. Factors that interfere with GHB (HbA1c) test results. Available from: http://www.ngsp.org/prog/factors.htm [last accessed on 2008 Dec 22]
3. Smaldone A. Glycemic control and hemoglobinopathy: When A1c may not be reliable. Diabetes Spectrum 2008;21:46-49
4. Malekiani CL, Ganesan A, Decker CF. Effect of Hemoglobinopathies on Hemoglobin A1c Measurements. Am J Med 2008;121:e5.
5. Wiwanitkit V. Problem of using hemoglobin A1c measurement in endemic area of hemoglobinopathy. Prim Care Diabetes 2007;1:173-175
6. Sacks DB. Hemoglobin variants and hemoglobin A1c analysis: Problems solved? Clin Chem 2003;49:1245-1247
7. Hb Var: A database of human hemoglobin variants and thalassemias. Available from: http://www.globin.cse.psu.edu/cgi-bin/hbvar/counter [last accessed on 2008 Dec 22]
8. Chu CH, Wang MC, Sun TH, Lee JK, Lam HC. Very low HbA1c values in a patient with clinical silent hemoglobin variant (Hemoglobin J): A case report. J Intern Med Taiwan 2007;18:45-50.
9. PK Nigam, S Sharma, R Sareen, V Paul, A Lal. Lack of precision in HbA1c values on Variant II in cases of Hemoglobin Q India. Indian J Clin Biochem 2006;21:72-75
10. Van den Ouweland JM, Van Daal H, Klaassen CH, Van Aarssen Y, Harteveld CL, Giordano PC. The silent hemoglobin alpha chain variant Hb Riccarton [alpha 51(CE9)Gly -> Ser] may affect HbA1c determination on the HLC-723 G7 analyzer. Clin Chem Lab Med 2008;46:827-830
11. Bry L, Chen PC, Sacks DB. Effects of hemoglobin variants and chemically modified derivatives on assays for glycohemoglobin. Clin Chem 2001;47:153-163
12. Sabath DE. Case study: Artifactually low HbA1c in a patient with high Hemoglobin F. Clin Diabetes 2000;18:179-183
13. Higgins T, Stewart D, Boehr E. Challenges in HbA1c analysis and reporting: An interesting case illustrating many pitfalls. Clin Biochem 2008;41:1104-1106
14. Little RR, Rohlfing CL, Hanson S, Connolly S, Higgins T, Weykamp CW, et al. Effects of Hemoglobin (Hb) E and Hb D traits on measurements of glycated hemoglobin (HbA1c) by 23 methods. Clin Chem 2008;54:1277-1282
15. Tsai LY, Tsai SM, Lin MN, Liu SF. Effect of hemoglobin variants (Hb J, Hb G and Hb E) on HbA1c values as measured by cation-exchange HPLC (Diamat). Clin Chem 2001;47:756-758
16. Pravatmuang P, Sae-Ngow B, Whanpuch T, Leowattana W. Effect of HbE and HbH on HbA1c level by ionicexchange HPLC comparing to immunoturbidimetry. Clin Chim Acta 2001;313:171-178
17. Roberts WL, Safar-Pour S, De BK, Rohlfing CL, Weykamp CW, Little RR. Effects of Hemoglobin C and Hemoglobin S traits on glycohemoglobin measurements by eleven methods. Clin Chem 2005;51:776-778
18. Roberts WL, De BK, Brown D, Hanbury CM, Hoyer JD, John WG, et al. Effects of Hemoglobin C and S traits on eight glycohemoglobin methods. Clin Chem 2002;48:383-385
19. Schnedl WJ, Lahousen T, Wallner SJ, Krause R, Lipp RW. Silent hemoglobin variants and determination of HbA1c with the high-resolution program of the HPLC HA-8160 hemoglobin analyzer. Clin Biochem 2005;38:88-91.
20. Lahousen T, Roller RE, Lipp RW, Schnedl WJ. Silent hemoglobin variants and determination of HbA1c with the HPLC Bio-Rad Variant II. J Clin Pathol 2002;55:699-703.