The purification of plasmid DNA for clinical trials using membrane chromatography

BioPharm International

Volumn 23, Issue 2, 2010, Pages 46-54

  Limonta, Miladys ^a   Marquez, Gabriel ^a   Pupo, Martha ^a   Ruiz, Odalys ^a  

^a CENTER FOR GENETIC ENGINEERING AND BIOTECHNOLOGY   (Cuba)

Author keywords

[No Author keywords available]

Indexed keywords

PLASMID DNA; RECOMBINANT PROTEIN; STRUCTURAL PROTEIN; VIRUS PROTEIN;

ANIMAL EXPERIMENT; ANION EXCHANGE CHROMATOGRAPHY; ARTICLE; BIOLOGICAL ACTIVITY; CELL LYSATE; CHROMATOGRAPHY; DNA IMMUNIZATION; DNA PURIFICATION; FEMALE; FLOW RATE; ION EXCLUSION CHROMATOGRAPHY; MEMBRANE CHROMATOGRAPHY; MOUSE; NONHUMAN;

EID: 77951490805     PISSN: 1542166X     EISSN: None     Source Type: Journal    
DOI: None     Document Type: Article

References (17)

1. Urthaler J, Schleg R, PogdornikA, Strancar A, Jungbauer A, Necina R. Application of Monoliths for plasmid DNA purification development and transfer to production. J Chrom A. 2005;1065: 93-106. (Pubitemid 40247841)
2. Ayazy P. Scaleable processes for the manufacture of therapeutic quantities of plasmid DNA. Biotech Appl Biochem. 2003;37:207-218
3. Stadler J, Lemmens R, NyhammarT. Plasmid DNA purification. J Gene Med. 2004;6:S54-S66. (Pubitemid 40228017)
4. Limonta M, MSrquez G, Rey I, Pupo M, Rufz 0, Amador-Canizares Y. Plasmid DNA recovery using size exclusion and perfusion chromatography. BioPharm Int. 2008;21:38-48.
5. Lemmens R, Olsson U, NyhammarT, Stadler J. Supercoiled plasmid DNA: selective purification by thiophilic/aromatic adsorption. J Chromatogr B. 2003;784:291-300. (Pubitemid 36044012)
6. US Food and Drug Administration. Guidance for Industry: Considerations for Plasmid DNA vaccines for Infectious Disease Indications. Rockville, MD: 2005 Feb.
7. Duenas-Carrera S, Morales J, Acosta-Rivero N, Lorenzo U, Garcfa C, Ramos T. Variable level expression of hepatitis C virus core protein in a prokaryotic system. Analysis of the humoral response in rabbit. Biotecnologfa Aplicada. 1999;16: 226-231 (Pubitemid 30020183)
8. Lorenzo U, Garcia O, Acosta-Rivero N, Duenas-Carrera S, Martinez G, Alvarez-Obregon J. Expression and immunological evaluation of the Escherichia coli-derived hepatitis C virus envelope El protein. Biotechnol Appl Biochem. 2000;32:137-143 (Pubitemid 32835806)
9. Martfnez-Donato G, CapdesunerY, Acosta-Rivero N, Rodrfguez A, Morales-Grillo J, Martinez E. Multimeric HCV E2 protein obtained from Pichia pastoris cells induces a strong immune response in mice. Mol Biotechnol. 2007;35:225-235 (Pubitemid 47233921)
10. Duenas-Carrera S, Alvarez-Lajonchere L, Alvarez- Obregón JC, Pérez A, Acosta-Rivero N, Vazquez DM. Enhancement of the immune response generated against hepatitis C virus enveloped proteins after DNA vaccination with polyprotein-encoding plasmids. Biotechnol Appl Biochem. 2002;35:205-212 (Pubitemid 34729115)
11. Acosta-Rivero N, Aguilera Y, Falcon V, Poutou J, Musacchio A, Alvarez-Lajonchere A. Uftrastructural and immunological characterization of hepatitis C core protein-DNA plasmid complexes. Am J Immunol. 2006;2:67-72.
12. Birnboim HC. A rapid alkaline extraction method for the isolation of plasmid DNA. Methods Enzymology. 1983;100:243-255 (Pubitemid 14001237)
13. Duval E, Burke G. Purification of pharmaceutical-grade plasmid DNA by anion-exchange chromatography in an RNase-free process. J Chromatogr B.2004;804:327-335 (Pubitemid 38481708)
14. Meacle FJ, Lander R, Ayazi Shamlou P, Titchener-Hooker NJ. Impact of Engineering Flow Conditions on Plasmid DNA Yield and Purity in Chemical Cell Lysis Operations. Biotechnol Bioeng. 2004;87:293-302. (Pubitemid 39078278)
15. Endres HN, Johnson JAC, Ross CA, Welp JK, Etzel MR. Evaluation of an ion-exchange membrane for the purification of plasmid DNA, Biotechnol Appl Biochem. 2003;37:259-266 (Pubitemid 36713033)
16. Zhang S, Krivosheyeva A, Nochumson S. Large scale capture and partial purification of plasmid DNA using anion-exchange membrane capsules. Biotechnol Appl Biochem. 2003;37:245-249 (Pubitemid 36713031)
17. Gottschalk U, Fischer-Fruehholz S, Reif 0. Membrane adsorbers-A cutting edge process technology at the threshold. Bioprocess Int. 2004 May;(5):56-65.