Plasmid DNA recovery using size-exclusion and perfusion chromatography

BioPharm International

Volumn 21, Issue 9, 2008, Pages 38-48

  Limonta, Miladys ^a   Márquez, Gabriel ^a   Rey, Isabel ^a   Pupo, Martha ^a   Ruiz, Odalys ^a   Amador Cañizares, Yalena ^a   Dueñas Carrera, Santiago ^a  

^a CENTER FOR GENETIC ENGINEERING AND BIOTECHNOLOGY   (Cuba)

Author keywords

[No Author keywords available]

Indexed keywords

HEPATITIS C ANTIBODY; HEPATITIS C ANTIGEN; ORGANIC SOLVENT; PLASMID DNA; POLYPROTEIN; RNA;

ALKALINITY; ANALYTIC METHOD; ANIMAL EXPERIMENT; ANIMAL MODEL; ANTIBODY DETECTION; ANTIBODY RESPONSE; ARTICLE; BACTERIAL CELL; CELL LYSATE; CENTRIFUGATION; CONTROLLED STUDY; CULTURE MEDIUM; CYTOLYSIS; DNA DETERMINATION; DNA PURIFICATION; ELUTION; ENZYME DEGRADATION; ESCHERICHIA COLI; FEMALE; GEL PERMEATION CHROMATOGRAPHY; HEPATITIS C; HEPATITIS C VIRUS; IMMUNOGENICITY; IN VIVO STUDY; MOLECULAR WEIGHT; MOUSE; NONHUMAN; RNA ISOLATION;

EID: 54949141519     PISSN: 1542166X     EISSN: None     Source Type: Journal    
DOI: None     Document Type: Article

References (16)

1. Sandberg L, Bjurling A, Busson P, Vasi J, Lemmens R. Thiophilic interaction chromatography for supercoiled plasmid DNA purification. J Biotechnol. 2004;109:193-199.
2. US Food and Drug Administration. Center for Biologics Evaluation and Research. Guidance for industry. Considerations for plasmid DNA vaccines for infectious disease indications. Rockville, MD: 2005 Feb.
3. Diogo M, Queiroz J, Monteiro G, Martins S, Ferreira G, Prazeres D. Purification of a cystic fibrosis plasmid vector for gene therapy using hydrophobic interaction chromatography. Biotechnol Bioeng. 2000;68:576-583.
4. Dueñas-Carrera S, Morales J, Acosta-Rivero N, Lorenzo LJ, García C, Ramos T, et al. Variable level expression of hepatitis C virus core protein in a prokaryotic system. Analysis of the humoral response in rabbit. Biotecnología Aplicada. 1999;16(4):226-231.
5. Lorenzo LJ, Garcia O, Acosta-Rivero N, Dueñas-Carrera S, Martinez G, Alvarez-Obregon J, et al. Expression and immunological evaluation of the Escherichia coli-derived hepatitis C virus envelope E1 protein. Biotechnol Appl Biochem. 2000;32:137-143.
6. Martínez-Donato G, Capdesuñer Y, Acosta-Rivero N, Rodríguez A, Morales-Grillo J, Martínez E, González M, et al. Multimeric HCV E2 protein obtained from Pichia pastoris cells induces a strong immune response in mice. Mol Biotechnol. 2007;35:225-35.
7. Dueñas-Carrera S. Alvarez-Lajonchere L, Alvarez-Obregón JC, Pérez A, Acosta-Rivero N, Vázquez DM, et al. Enhancement of the immune response generated against hepatitis C virus envelope proteins after DNA vaccination with polyprotein-encoding plasmids. Biotechnol Appl Biochem. 2002;35:205-212.
8. Lis JT, Schleif R. Size fractionation of double stranded DNA by precipitation with polyethylene glycol. Nucleic Acids Res. 1975;2:383-389.
9. Horn NA, Meek JA, Budahazi G, Marquet M. Cancer gene therapy using plasmid DNA: purification of DNA for human clinical trials. Human Gene Ther. 1995;6:565-573.
10. Ayazy P. Scaleable processes for the manufacture of therapeutic quantities of plasmid DNA. Biotech Appl Biochem. 2003;37:207-218.
11. Ferreira GNM, Cabral JMS, Prazeres DMF. Development of process flow sheets for the purification of supercoiled plasmids for gene therapy applications. Biotechnol Prog. 1999;15:725-731.
12. Lemmens R, Olsson U, Nyhammar T, Stadler J. Supercoiled plasmid DNA: selective purification by thiophilic/aromatic adsorption. J Chromatogr B. 2003;784:291-300.
13. Afeyan NB. Gordon NF, Mazsaroff I, Varady L, Pulton SP. Flow-through particles for the high performance liquid chromatographic separation of biomolecules: perfusion chromatography. J Chromatogr. 1990;519:1-29.
14. Sambrook J, Fritsch EF, Maniatis M. Cloning: a laboratory manual. 2nd ed. Cold Spring Harbor Laboratory, ed. Cold Spring Harbor, New York; 1989.
15. Stadler J, Lemmens R, Nyhammar T. Plasmid DNA purification. J Gene Med. 2004;6:S54-S66.
16. Eon-Duval A, Burke G. Purification of pharmaceutical-grade plasmid DNA by anion-exchange chromatography in an RNase-free process. J Chromatogr B. 2004;804:327-335.