Purification and characterization of a thermostable intra-cellular β-glucosidase with transglycosylation properties from filamentous fungus Termitomyces clypeatus

Bioresource Technology

Volumn 101, Issue 7, 2010, Pages 2412-2420

  Pal, Swagata ^a   Banik, Samudra Prosad ^a   Ghorai, Shakuntala ^a   Chowdhury, Sudeshna ^a   Khowala, Suman ^a  

^a INDIAN INSTITUTE OF CHEMICAL BIOLOGY   (India)

Author keywords

2 Deoxy D glucose; Co aggregation; Intra cellular glucosidase; Termitomyces clypeatus; Transglycosylation

Indexed keywords

BIOHAZARDS; CHEMICAL HAZARDS; CHROMATOGRAPHIC ANALYSIS; CHROMATOGRAPHY; CIRCULAR DICHROISM SPECTROSCOPY; ENZYMES; GLYCOSYLATION; ION CHROMATOGRAPHY; ION EXCHANGE; PURIFICATION;

2-DEOXY-D-GLUCOSE; CELLO-OLIGOSACCHARIDES; GLUCOSIDASE; HELICAL CONFORMATION; ION EXCHANGE CHROMATOGRAPHY; TERMITOMYCES CLYPEATUS; TRANS GLYCOSYLATION ACTIVITY; TRANSGLYCOSYLATION;

ENZYME ACTIVITY;

4 NITROPHENYLGLUCOPYRANOSIDE; BETA GLUCOSIDASE; CELLOBIOSE; DEOXYGLUCOSE; GLUCOPYRANOSIDE; GLUCOSE; GLYCOSIDE; OLIGOSACCHARIDE; SUCRASE; UNCLASSIFIED DRUG; AMINO ACID; METAL;

BIOACTIVITY; ENZYME ACTIVITY; FUNGUS; HOMOGENEITY; ION EXCHANGE; LIQUID CHROMATOGRAPHY; PH; TEMPERATURE EFFECT;

ALPHA HELIX; ARTICLE; BIOSAFETY; CIRCULAR DICHROISM; ENZYME ACTIVITY; ENZYME ANALYSIS; ENZYME CONFORMATION; ENZYME GLYCOSYLATION; ENZYME PURIFICATION; ENZYME SPECIFICITY; ENZYME STABILITY; ENZYME SYNTHESIS; FUNGAL STRAIN; GEL FILTRATION CHROMATOGRAPHY; HIGH PERFORMANCE LIQUID CHROMATOGRAPHY; IMMUNOBLOTTING; ION EXCHANGE CHROMATOGRAPHY; MATRIX ASSISTED LASER DESORPTION IONIZATION TIME OF FLIGHT MASS SPECTROMETRY; MOLECULAR WEIGHT; MYCELIUM; NONHUMAN; PH; POLYACRYLAMIDE GEL ELECTROPHORESIS; PRIORITY JOURNAL; TERMITOMYCES CLYPEATUS; THERMOSTABILITY; BIOSYNTHESIS; CHEMISTRY; DRUG EFFECT; ENZYMOLOGY; GLYCOSYLATION; INTRACELLULAR SPACE; ISOLATION AND PURIFICATION; KINETICS; METABOLISM; SEQUENCE ANALYSIS; TEMPERATURE; TERMITOMYCES;

FUNGI; TERMITOMYCES CLYPEATUS;

AMINO ACIDS; BETA-GLUCOSIDASE; CIRCULAR DICHROISM; ELECTROPHORESIS, POLYACRYLAMIDE GEL; ENZYME STABILITY; GLYCOSYLATION; HYDROGEN-ION CONCENTRATION; INTRACELLULAR SPACE; KINETICS; METALS; MOLECULAR WEIGHT; SEQUENCE ANALYSIS, PROTEIN; SUBSTRATE SPECIFICITY; TEMPERATURE; TERMITOMYCES;

EID: 77951243280     PISSN: 09608524     EISSN: None     Source Type: Journal    
DOI: 10.1016/j.biortech.2009.11.064     Document Type: Article

References (35)

1. Andya, J.D., Hsu, C.C., Shire, S.J., 2003. Mechanisms of aggregate formation and carbohydrate excipient stabilization of lyophilized humanized monoclonal antibody formulations. AAPS Pharm. Sci. 5 (2), E10.
2. Bhatia, Y., Mishra, S., Bisaria, V.S., 2002. Microbial β-glucosidases: cloning, properties, and applications. Crit. Rev. Biotechnol. 22, 375-407.
3. Bhiri, F., Chaabouni, S.E., Limam, F., Ghrir, R., Marzouki, N., 2008. Purification and characterization of extracellular β-glucosidase from the hypercellulytic Pol 6 mutant of Penicillium occitanis. Appl. Biochem. Biotechnol. 149, 169- 182.
4. Blake, M.S., Johnston, K.H., Russell-Jones, G.J., Gotschlich, E.C., 1984. A rapid, sensitive method for detection of alkaline phosphatase-conjugated antiantibody on Western blots. Anal. Biochem. 136, 175-179.
5. Chirico, W.J., Brown Jr., R.D., 1987. Purification and characterization of a bglucosidase from Trichoderma reesei. Eur. J. Biochem. 165, 333-341.
6. Chowdhury, S., Ghorai, S., Banik, S.P., Pal, S., Basak, S., Khowala, S., 2009. Characterization of a novel low molecular weight sucrase from filamentous fungus Termitomyces clypeatus. Process. Biochem. 44, 1075-1082.
7. Cromwell, M.E.M., Hilario, E., Jacobson, F., 2006. Protein aggregation and bioprocessing. AAPS J. 8, E572-E579.
8. Datema, R., Schwarz, R.T., Rivas, L.A., Lezica, R.P., 1983. Inhibition of fl-1, 4-glucan biosynthesis by deoxyglucose. The effect on the glucosylation of lipid intermediates. Plant Physiol. 71, 76-81.
9. Dhake, A.B., Patil, M.B., 2005. Production of β-glucosidase by Penicillium purpurogenum. Brazil J. Microbiol. 36, 170-176.
10. Ducret, A., Trani, M., Lortie, R., 2002. Screening of various glycosidases for the synthesis of octyl glucoside. Biotechnol. Bioeng. 77, 752-757.
11. González-Pombo, P., Pérez, G., Carrau, F., Guisán, J.M., Batista-Viera, F., Brena, B.M., 2008. One-step purification and characterization of an intracellular bglucosidase from Metschnikowia pulcherrima. Biotechnol. Lett. 30, 1469- 1475.
12. Goyal, K., Kim, B.J., Kim, J.D., Kim, Y.K., Kitaoka, M., Hayashi, K., 2002. Enhancement of transglycosylation activity by construction of chimeras between mesophilic and thermophilic β-glucosidase. Arch. Biochem. Biophys. 407, 125-134.
13. Gueguen, Y., Chemardin, P., Arnaud, A., Galzy, P., 1995. Purification and characterization of an intracellular β-glucosidase from β-glucosidases Botrytis cinerea. Enzyme Microbiol. Technol. 17, 900-906.
14. Gueguen, Y., Chemardin, P., Labrot, P., Arnaud, A., Galzy, P., 1997. Purification and characterization of an intracellular β-glucosidase from a new strain Leuconostoc mesenteroides isolated from cassava. J. Appl. Microbiol. 82, 469-476.
15. Inglin, M., Feinberg, B.A., Loewenberg, J.R., 1980. Partial purification and characterization of a new intracellular β-glucosidase of Trichoderma reesei. Biochem. J. 185, 515-519.
16. Kaur, J., Chadha, B.S., Kumar, B.A., Kaur, G.S., Saini, H.S., 2007. Purification and characterization of β-glucosidase from Melanocarpus sp. MTCC 3922. Electron. J. Biotechnol. 10, ISSN: 0717-3458.
17. Khowala, S., Ghosh, A.K., Sengupta, S., 1992. Saccharification of xylan by an amyloglucosidase of Termitomyces clypeatus and synergism in the presence of xylanase. Appl. Microbiol. Biotechnol. 37, 287-292.
18. Kuo, S.C., Lampen, J.O., 1972. Inhibition by 2-deoxy-D-glucose of synthesis of glycoprotein enzymes by protoplasts of Saccharomyces: relation to inhibition of sugar uptake and metabolism. J. Bacteriol. 111, 419-429.
19. Laemmli, U.K., 1970. Cleavage of structural proteins during the assembly of the head of bacteriophage T4. Nature 227, 680-685.
20. Meyer, H.P., Canevascini, G., 1981. Separation and some properties of two intracellular β-glucosidases of Sporotrichum (Chrysosporium) thermophile. Appl. Environ. Microbiol. 41, 924-931.
21. Miller, G.L., 1959. Use of dinitrosalicylic acid regent for determination of reducing sugars. Anal. Chem. 31, 426-428.
22. Mukherjee, S., Basak, S., Khowala, S., 2001. Hetero-aggregation with sucrase affects the activity, stability and conformation of extra-and intra-cellular cellobiase in the filamentous fungus Termitomyces clypeatus. Enzyme Microbiol. Technol. 29, 213-224.
23. Mukherjee, S., Chowdhury, S., Ghorai, S., Pal, S., Khowala, S., 2006. Cellobiase from Termitomyces clypeatus: activity and secretion in presence of glycosylation inhibitors. Biotechnol. Lett. 28, 1773-1778.
24. Mukherjee, S., Khowala, S., 2002. Secretion of cellobiase is mediated via vacuoles in Termitomyces clypeatus. Biotechnol. Prog. 18, 1195-1200.
25. Nakkharat, P., Haltrich, D., 2006. Purification and characterisation of an intracellular enzyme with β-glucosidase and β-galactosidase activity from the thermophilic fungus Talaromyces thermophilus CBS 236.58. J. Biotechnol. 123, 304-313.
26. Park, T.H., Choi, K.W., Park, C.S., Lee, S.B., Kang, H.Y., Shon, K.J., Park, J.S., Cha, J., 2005. Substrate specificity and transglycosylation catalyzed by a thermostable bglucosidase from marine hyperthermophile Thermotoga neapolitana. Appl. Microbiol. Biotechnol. 69, 411-422.
27. Parry, N.J., Beever, D.E., Owen, E., Vandenberghe, I., Beeumen, J.V., Bhat, M.K., 2001. Biochemical characterization and mechanism of action of a thermostable bglucosidase purified from Thermoascus aurantiacus. Biochem. J. 353, 117- 127.
28. Rajoka, M., Khan, S., 2005. Hyper-production of a thermotolerant β-xylosidase by a deoxy-D-glucose and cycloheximide resistant mutant derivative of Kluyveromyces marxianus. Electron. J. Biotechnol. 8, 0717-3458.
29. Ralsera, M., Wamelinkb, M.M., Struysb, E.A., Joppicha, C., Krobitscha, S., Jakobsb, C., Lehracha, H., 2008. A catabolic block does not sufficiently explain how 2-deoxy-D-glucose inhibits cell growth. PNAS 105, 17807-17811.
30. Skory, C.D., Freer, S.N., Bothast, R.J., 1996. Properties of an intracellular β-glucosidase purified from the cellobiose-fermenting yeast Candida wickerhamii. Appl. Microbiol. Biotechnol. 46, 353-359.
31. 3H dual labelling and isopycnic centrifugation in caesium trifluoroacetate. Biochem. J. 327, 699-708.
32. Umekawa, M., Huang, W., Li, B., Kiyotaka, F., Ashida, H., Wang, L.X., Yamamoto, K., 2008. Mutants of Mucor hiemalis endo-N-acetylglucosaminidase show enhanced tansglycosylation and glycosynthase-like activities. J. Biol. Chem. 283, 4469-4479.
33. Umile, C., Kubicek, C.P., 1986. A constitutive, plasma membrane-bound β-glucosidase in Trichoderma reesei. FEMS Microbiol. Lett. 34, 291-295.
34. Yang, S., Jiang, Z., Yan, Q., Zhu, H., Yang, S., Jiang, Z., Yan, Q., Zhu, M.H., 2007. Characterization of a thermostable extracellular β-glucosidase with activities of exoglucanase and transglycosylation from Paecilomyces thermophila. J. Agric. Food Chem. 56, 602-608.
35. Yazdi, M.T., Khosravi, A.A., Nemati, M., Motlagh, M.D.V., 2003. Purification and characterization of two intracellular β-glucosidases from the Neurospora crassa mutant cell-1. World. J. Microbiol. Biotechnol. 19, 79-84.