Quality of cell products: Authenticity, identity, genomic stability and status of differentiation

Transfusion Medicine and Hemotherapy

Volumn 37, Issue 2, 2010, Pages 57-64

  Dittmar, Kurt E J ^a   Simann, Meike ^a   Zghoul, Nadia ^a   Schön, Oliver ^a   Meyring, Wilhelm ^b   Hannig, Horst ^b   MacKe, Lars ^a   Dirks, Wilhelm G ^c   Miller, Konstantin ^d   Garritsen, Henk S P ^b   Lindenmaier, Werner ^a  

^a HELMHOLTZ CENTRE FOR INFECTION RESEARCH   (Germany)

^b KLINIKUM BRAUNSCHWEIG   (Germany)

^c LEIBNIZ INSTITUTE DSMZ GERMAN COLLECTION OF MICROORGANISMS AND CELL CULTURES   (Germany)

^d HANNOVER MEDICAL SCHOOL   (Germany)

Author keywords

Cell identity; Cross contamination; DNA typing; Karyotype; Primary human cells

Indexed keywords

MITOCHONDRIAL DNA;

CELL DIFFERENTIATION; CELL LINE; CELL MATURATION; CELL THERAPY; DNA DETERMINATION; GENOMIC INSTABILITY; GENOTYPE; HUMAN; KARYOTYPING; MICROSATELLITE INSTABILITY; NERVE CELL; OSTEOSARCOMA CELL; PHENOTYPE; POLYMERASE CHAIN REACTION; REVIEW; SHORT TANDEM REPEAT; STEM CELL;

EID: 77951096390     PISSN: 16603796     EISSN: None     Source Type: Journal    
DOI: 10.1159/000284401     Document Type: Review

References (68)

1. Cheng L, Xiao L, Zeng F, Zhang YA: Stem cells shine in Shanghai. Cell Stem Cell 2008;2:34-37.
2. Hay RJ: Cell Quantitation and characterization; in Atala A, Lanza RP (eds): Methods of Tissue Engineering. San Diego, Academic Press, 2002, pp 55-84.
3. EU: Regulation (EC) No 1394/2007 of the European Parliament and of the Council of 13 November 2007 on Advanced Therapy Medicinal Products and Amending Directive 2001/83/EC and Regulation (EC) No 726/2004. Official Journal of the European Union 2007;121-137.
4. Stacey GN: Cell contamination leads to inaccurate data: we must take action now. Nature 2000; 403:356.
5. Stacey GN: Standardisation of cell lines. Dev Biol (Basel) 2002;111:259-272
6. Hughes P, Marshall D, Reid Y, Parkes H, Gelber C: The costs of using unauthenticated, over-passaged cell lines: how much more data do we need? Biotechniques 2007;43:575, 577-778, 581-582.
7. MacLeod RA, Dirks WG, Matsuo Y, Kaufmann M, Milch H, Drexler HG: Widespread intraspecies cross-contamination of human tumor cell lines arising at source. Int J Cancer 1999;83:555-563.
8. Markovic O, Markovic N: Cell cross-contamination in cell cultures: the silent and neglected danger. In Vitro Cell Dev Biol Anim 1998;34:1-8.
9. Takahashi K, Sawasaki Y, Hata J, Mukai K, Goto T: Spontaneous transformation and immortalization of human endothelial cells. In Vitro Cell Dev Biol 1990;26:265-274.
10. Dirks WG, MacLeod RA, Drexler HG: ECV304 (endothelial) is really T24 (bladder carcinoma): cell line cross- contamination at source. In Vitro Cell Dev Biol Anim 1999;35:558-559.
11. Editorial: Identity crisis. Nature 2009;457:935-936.
12. Chatterjee R: Cell biology. Cases of mistaken identity. Science 2007;315:928-931.
13. Drexler HG, Dirks WG, MacLeod RA: False human hematopoietic cell lines: cross-contaminations and misinterpretations. Leukemia 1999;13: 1601-1607.
14. Nims RW, Shoemaker AP, Bauernschub MA, Rec LJ, Harbell JW: Sensitivity of isoenzyme analysis for the detection of interspecies cell line cross-contamination. In Vitro Cell Dev Biol Anim 1998;34: 35-39.
15. Drexler HG, Matsuo AY, MacLeod RA: Continuous hematopoietic cell lines as model systems for leukemia-lymphoma research. Leuk Res 2000;24:881-911.
16. Bartlett SE, Davidson WS: FINS (forensically informative nucleotide sequencing): a procedure for identifying the animal origin of biological specimens. Biotechniques 1992;12:408-411.
17. Unseld M, Beyermann B, Brandt P, Hiesel R: Identification of the species origin of highly processed meat products by mitochondrial DNA sequences. PCR Methods Appl 1995;4:241-243.
18. Cann RL, Stoneking M, Wilson AC: Mitochondrial DNA and human evolution. Nature 1987;325:31-36.
19. Di Rienzo A, Wilson AC: Branching pattern in the evolutionary tree for human mitochondrial DNA. Proc Natl Acad Sci U S A 1991;88:1597-1601.
20. Vigilant L, Stoneking M, Harpending H, Hawkes K, Wilson AC: African populations and the evolution of human mitochondrial DNA. Science 1991; 253:1503-1507.
21. Bonne-Tamir B, Johnson MJ, Natali A, Wallace DC, Cavalli-Sforza LL: Human mitochondrial DNA types in two Israeli populations - a comparative study at the DNA level. Am J Hum Genet 1986;38:341-351.
22. Harihara S, Hirai M, Suutou Y, Shimizu K, Omoto K: Frequency of a 9-bp deletion in the mitochondrial DNA among Asian populations. Hum Biol 1992;64:161-166.
23. Torroni A, Brown MD, Lott MT, Newman NJ, Wallace DC: African, Native American, and European mitochondrial DNAs in Cubans from Pinar del Rio Province and implications for the recent epidemic neuropathy in Cuba. Cuba Neuropathy Field Investigation Team. Hum Mutat 1995;5:310-317.
24. Gill P, Ivanov PL, Kimpton C, Piercy R, Benson N, Tully G, Evett I, Hagelberg E, Sullivan K: Identification of the remains of the Romanov family by DNA analysis. Nat Genet 1994;6:130-135.
25. Holland MM, Fisher DL, Mitchell LG, Rodriquez WC, Canik JJ, Merril CR, Weedn VW: Mitochondrial DNA sequence analysis of human skeletal remains: identification of remains from the Vietnam War. J Forensic Sci 1993;38:542-553.
26. Parsons TJ, Muniec DS, Sullivan K, Woodyatt N, Alliston-Greiner R, Wilson MR, Berry DL, Holland KA, Weedn VW, Gill P, Holland MM: A high observed substitution rate in the human mitochondrial DNA control region. Nat Genet 1997;15:363-368.
27. Garritsen HS, Szuflad P, Sibrowski W, Dzik WH: A sequence-specific polymerase chain reaction assay for mitochondrial DNA polymorphisms in human platelets and white cells. Transfusion 1997;37: 1012-1019.
28. Garritsen HS, Hoerning A, Hellenkamp F, Cassens U, Mittmann K, Sibrowski W: Polymorphisms in the non-coding region of the human mitochondrial genome in unrelated plateletapheresis donors. Br J Haematol 2001;112:995-1003.
29. Shadel GS, Clayton DA: Mitochondrial DNA maintenance in vertebrates. Annu Rev Biochem 1997;066:409-435.
30. Clayton DA, Doda JN, Friedberg EC: The absence of a pyrimidine dimer repair mechanism in mammalian mitochondria. Proc Natl Acad Sci U S A 1974;71:2777-2781.
31. Enzmann H, Kuhlem C, Loser E, Bannasch P: Damage to mitochondrial DNA induced by the hepatocarcinogen diethylnitrosamine in ovo. Mutat Res 1995;329:113-120.
32. Shen CC, Wertelecki W, Driggers WJ, LeDoux SP, Wilson GL: Repair of mitochondrial DNA damage induced by bleomycin in human cells. Mutat Res 1995;337:19-23.
33. Wallace DC, Shoffner JM, Trounce I, Brown MD, Ballinger SW, Corral-Debrinski M, Horton T, Jun AS, Lott MT: Mitochondrial DNA mutations in human degenerative diseases and aging. Biochim Biophys Acta 1995;1271:141-151.
34. Anderson S, Bankier AT, Barrell BG, de Bruijn MH, Coulson AR, Drouin J, Eperon IC, Nierlich DP, Roe BA, Sanger F, Schreier PH, Smith AJ, Staden R, Young IG: Sequence and organization of the human mitochondrial genome. Nature 1981;290:457-465.
35. Andrews RM, Kubacka I, Chinnery PF, Lightowlers RN, Turnbull DM, Howell N: Reanalysis and revision of the Cambridge reference sequence for human mitochondrial DNA. Nat Genet 1999; 23:147.
36. Hutter G, Nickenig C, Garritsen H, Hellenkamp F, Hoerning A, Hiddemann W, Dreyling M: Use of polymorphisms in the noncoding region of the human mitochondrial genome to identify potential contamination of human leukemia-lymphoma cell lines. Hematol J 2004;5:61-68.
37. Dirks WG, Faehnrich S, Estella IA, Drexler HG: Short tandem repeat DNA typing provides an international reference standard for authentication of human cell lines. ALTEX 2005;22:103-109.
38. Masters JR, Thomson JA, Daly-Burns B, Reid YA, Dirks WG, Packer P, Toji LH, Ohno T, Tanabe H, Arlett CF, Kelland LR, Harrison M, Virmani A, Ward TH, Ayres KL, Debenham PG: Short tandem repeat profiling provides an international reference standard for human cell lines. Proc Natl Acad Sci U S A 2001;98:8012-8017.
39. Shaffer L, Tommerup N: ISCN 2005: An International System for Human Cytogenetic Nomenclature. Basel: Karger, 2009.
40. Brimble SN, Zeng X, Weiler DA, Luo Y, Liu Y, Lyons IG, Freed WJ, Robins AJ, Rao MS, Schulz TC: Karyotypic stability, genotyping, differentiation, feeder-free maintenance, and gene expression sampling in three human embryonic stem cell lines derived prior to August 9, 2001. Stem Cells Dev 2004;13:585-597.
41. Caisander G, Park H, Frej K, Lindqvist J, Bergh C, Lundin K, Hanson C: Chromosomal integrity maintained in five human embryonic stem cell lines after prolonged in vitro culture. Chromosome Res 2006;14:131-137.
42. Grimes BR, Steiner CM, Merfeld-Clauss S, Traktuev DO, Smith D, Reese A, Breman AM, Thurston VC, Vance GH, Johnstone BH, Slee RB, March KL: Interphase FISH demonstrates that human adipose stromal cells maintain a high level of genomic stability in long-term culture. Stem Cells Dev 2009;18:717-724.
43. Lange C, Cakiroglu F, Spiess AN, Cappallo-Obermann H, Dierlamm J, Zander AR: Accelerated and safe expansion of human mesenchymal stromal cells in animal serum-free medium for transplantation and regenerative medicine. J Cell Physiol 2007;213:18-26.
44. Meza-Zepeda LA, Noer A, Dahl JA, Micci F, Myklebost O, Collas P: High-resolution analysis of genetic stability of human adipose tissue stem cells cultured to senescence. J Cell Mol Med 2008; 12:553-563.
45. Mitalipova MM, Rao RR, Hoyer DM, Johnson JA, Meisner LF, Jones KL, Dalton S, Stice SL: Preserving the genetic integrity of human embryonic stem cells. Nat Biotechnol 2005;23:19-20. (Pubitemid 41724618)
46. Loring JF, Rao MS: Establishing standards for the characterization of human embryonic stem cell lines. Stem Cells 2006;24:145-150.
47. Spits C, Mateizel I, Geens M, Mertzanidou A, Staessen C, Vandeskelde Y, Van der EJ, Liebaers I, Sermon K: Recurrent chromosomal abnormalities in human embryonic stem cells. Nat Biotechnol 2008;26:1361-1363.
48. Schrock E, du MS, Veldman T, Schoell B, Wienberg J, Ferguson-Smith MA, Ning Y, Ledbetter DH, Bar-Am I, Soenksen D, Garini Y, Ried T: Multicolor spectral karyotyping of human chromosomes. Science 1996;273:494-497.
49. Speicher MR, Carter NP: The new cytogenetics: blurring the boundaries with molecular biology. Nat Rev Genet 2005;6:782-792.
50. Urban AE, Korbel JO, Selzer R, Richmond T, Hacker A, Popescu GV, Cubells JF, Green R, Emanuel BS, Gerstein MB, Weissman SM, Snyder M: High-resolution mapping of DNA copy alterations in human chromosome 22 using high-density tiling oligonucleotide arrays. Proc Natl Acad Sci U S A 2006;103:4534-4539.
51. Peltomaki PT: Genetic basis of hereditary nonpolyposis colorectal carcinoma (HNPCC). Ann Med 1994;26:215-219.
52. Soong R, Anuar D, Liu Y, Eu KW, Han HC, Salto- Tellez M, Iacopetta B: Denaturing high performance liquid chromatography for the detection of microsatellite instability using Bethesda and Pentaplex marker panels. Diagn Mol Pathol 2008;17:127-133.
53. Darnfors C, Flodin A, Andersson K, Caisander G, Lindqvist J, Hyllner J, Wahlstrom J, Sartipy P: High-resolution analysis of the subtelomeric regions of human embryonic stem cells. Stem Cells 2005;23:483-488. (Pubitemid 40478487)
54. Gordeeva OF, Mitalipov S: Pluripotent stem cells: maintenance of genetic and epigenetic stability and prospects of cell technologies (in Russian). Ontogenez 2008;39:405-419.
55. Cowan CA, Klimanskaya I, McMahon J, Atienza J, Witmyer J, Zucker JP, Wang S, Morton CC, McMahon AP, Powers D, Melton DA: Derivation of embryonic stem-cell lines from human blastocysts. N Engl J Med 2004;350:1353-1356.
56. Draper JS, Smith K, Gokhale P, Moore HD, Maltby E, Johnson J, Meisner L, Zwaka TP, Thomson JA, Andrews PW: Recurrent gain of chromosomes 17q and 12 in cultured human embryonic stem cells. Nat Biotechnol 2004;22:53-54.
57. Inzunza J, Sahlen S, Holmberg K, Stromberg AM, Teerijoki H, Blennow E, Hovatta O, Malmgren H: Comparative genomic hybridization and karyotyping of human embryonic stem cells reveals the occurrence of an isodicentric X chromosome after long-term cultivation. Mol Hum Reprod 2004;10: 461-466.
58. Lefort N, Feyeux M, Bas C, Feraud O, Naceur-Griscelli A, Tachdjian G, Peschanski M, Perrier AL: Human embryonic stem cells reveal recurrent genomic instability at 20q11.21. Nat Biotechnol 2008; 26:1364-1366.
59. Maitra A, Arking DE, Shivapurkar N, Ikeda M, Stastny V, Kassauei K, Sui G, Cutler DJ, Liu Y, Brimble SN, Noaksson K, Hyllner J, Schulz TC, Zeng X, Freed WJ, Crook J, Abraham S, Colman A, Sartipy P, Matsui S, Carpenter M, Gazdar AF, Rao M, Chakravarti A: Genomic alterations in cultured human embryonic stem cells. Nat Genet 2005;37:1099-1103. (Pubitemid 41486662)
60. Rosler ES, Fisk GJ, Ares X, Irving J, Miura T, Rao MS, Carpenter MK: Long-term culture of human embryonic stem cells in feeder-free conditions. Dev Dyn 2004;229:259-274. (Pubitemid 38140465)
61. Dahl JA, Duggal S, Coulston N, Millar D, Melki J, Shahdadfar A, Brinchmann JE, Collas P: Genetic and epigenetic instability of human bone marrow mesenchymal stem cells expanded in autologous serum or fetal bovine serum. Int J Dev Biol 2008;52: 1033-1042.
62. Rubio D, Garcia-Castro J, Martin MC, de la FR, Cigudosa JC, Lloyd AC, Bernad A: Spontaneous human adult stem cell transformation. Cancer Res 2005;65:3035-3039.
63. Rubio D, Garcia S, Paz MF, De la Cueva T, Lopez- Fernandez LA, Lloyd AC, Garcia-Castro J, Bernad A: Molecular characterization of spontaneous mesenchymal stem cell transformation. PLoS One 2008;3:e1398.
64. Zhang ZX, Guan LX, Zhang K, Wang S, Cao PC, Wang YH, Wang Z, Dai LJ: Cytogenetic analysis of human bone marrow-derived mesenchymal stem cells passaged in vitro. Cell Biol Int 2007;31:645-648.
65. Bernardo ME, Zaffaroni N, Novara F, Cometa AM, Avanzini MA, Moretta A, Montagna D, Maccario R, Villa R, Daidone MG, Zuffardi O, Locatelli F: Human bone marrow derived mesenchymal stem cells do not undergo transformation after long-term in vitro culture and do not exhibit telomere maintenance mechanisms. Cancer Res 2007;67:9142-9149.
66. Miura Y, Gao Z, Miura M, Seo BM, Sonoyama W, Chen W, Gronthos S, Zhang L, Shi S: Mesenchymal stem cell-organized bone marrow elements: an alternative hematopoietic progenitor resource. Stem Cells 2006;24:2428-2436.
67. Buwe A, Steinlein C, Koehler MR, Bar-Am I, Katzin N, Schmid M: Multicolor spectral karyotyping of rat chromosomes. Cytogenet Genome Res 2003;103:163-168.
68. Beresford JN: Osteogenic stem cells and the stromal system of bone and marrow. Clin Orthop Relat Res 1989;240:270-280.