Caspase-3 knock-down reverses contractile dysfunction induced by sepsis in adult rat ventricular myocytes

British Journal of Pharmacology

Volumn 160, Issue 1, 2010, Pages 93-100

  Chopra, Mani ^a   Das, Padmalaya ^b   Sharma, Avadhesh C ^a,b  

^a TEXAS A AND M HEALTH SCIENCE CENTER   (United States)

^b PHILADELPHIA COLLEGE OF OSTEOPATHIC MEDICINE   (United States)

Author keywords

Cardiomyocyte; Caspase 3 siRNA; Confocal microscopy; Contractility; Polymicrobial sepsis; TUNEL

Indexed keywords

ALPHA ACTIN; CASPASE 3; MYOSIN LIGHT CHAIN; NORADRENALIN; SMALL INTERFERING RNA; TROPOMYOSIN;

ANIMAL CELL; ANIMAL EXPERIMENT; ANIMAL MODEL; ANIMAL TISSUE; ARTICLE; CONTROLLED STUDY; DISEASE EXACERBATION; DNA FRAGMENTATION; DOWN REGULATION; ENZYME ACTIVITY; GENE FUNCTION; GENETIC TRANSFECTION; HEART ARRHYTHMIA; HEART MUSCLE CELL; HEART MUSCLE CONTRACTILITY; HEART VENTRICLE; IMMUNOBLOTTING; IMMUNOCYTOCHEMISTRY; ISOLATED HEART; MALE; NONHUMAN; PRIORITY JOURNAL; RAT; SEPSIS; SHAM PROCEDURE; SPRAGUE DAWLEY RAT; UPREGULATION;

ANIMALS; CASPASE 3; CELL SIZE; CONTRACTILE PROTEINS; DNA FRAGMENTATION; GENE EXPRESSION REGULATION; GENE KNOCKDOWN TECHNIQUES; MALE; MYOCARDIAL CONTRACTION; MYOCYTES, CARDIAC; NOREPINEPHRINE; RATS; RATS, SPRAGUE-DAWLEY; RNA, SMALL INTERFERING; SEPSIS; TRANSFECTION;

EID: 77950955272     PISSN: 00071188     EISSN: 14765381     Source Type: Journal    
DOI: 10.1111/j.1476-5381.2010.00686.x     Document Type: Article

References (37)

1. Alexander SPH, Mathie A, Peters JA (2009). Guide to Receptors and Channels (GRAC), 4th edn. Br J Pharmacol 158 (Suppl. 1 S1 S254.
2. Bertolotto C, Ricci JE, Luciano F, Mari B, Chambard JC, Auberger P (2000). Cleavage of the serum response factor during death receptor-induced apoptosis results in an inhibition of the c-FOS promoter transcriptional activity. J Biol Chem 275 : 12941 12947. (Pubitemid 30241451)
3. Black SC, Huang JQ, Rezaiefar P, Radinovic S, Eberhart A, Nicholson DW et al. (1998). Colocalization of the cysteine protease caspase-3 with apoptotic myocytes after in vivo myocardial ischemia and reperfusion in the rat. J Mol Cell Cardiol 30 : 733 742. (Pubitemid 28190717)
4. Carlson DL, Maass DL, White J, Sikes P, Horton JW (2007). Caspase inhibition reduces cardiac myocyte dyshomeostasis and improves cardiac contractile function after major burn injury. J Appl Physiol 103 : 323 330. (Pubitemid 47092307)
5. Chang J, Wei L, Otani T, Keith AY, Entman M, Schwartz R (2003). Inhibitory cardiac transcription factor, SRF-N, is generated by caspase-3 cleavage in human heart failure and attenuated by ventricular unloading. Circulation 108 : r29 r35.
6. Cheng EH, Kirsch DG, Clem RJ, Ravi R, Kastan MB, Bedi A et al. (1997). Conversion of BCl-2 to a Bax-like death effector by caspases. Science 278 : 1966 1968.
7. Chopra M, Sharma AC (2007a). Caspase-3 inhibition augments norepinephrine-mediated contractile response in adult rat ventricular myocytes (ARVM) during sepsis. FASEB J 21 : 726.
8. Chopra M, Sharma AC (2007b). Distinct cardiodynamic and molecular characteristics during early and late stages of sepsis-induced myocardial dysfunction. Life Sci 81 : 306 316. (Pubitemid 47030576)
9. Chopra M, Sharma AC (2009). Contractile response of norepinephrine is modulated by caspase-3 in adult rat ventricular myocytes isolated from septic rat heart. Pharmacol Res 60 : 303 313.
10. Communal C, Sumandea M, de Tombe P, Narula J, Solaro RJ, Hajjar RJ (2002). Functional consequences of caspase activation in cardiac myocytes. Proc Natl Acad Sci USA 99 : 6252 6256. (Pubitemid 34493298)
11. Drewett V, Devitt A, Saxton J, Portman N, Greaney P, Cheong NE et al. (2001). Serum response factor cleavage by caspases 3 and 7 linked to apoptosis in human BJAB cells. J Biol Chem 276 : 33444 33451. (Pubitemid 37384553)
12. Fernandes CJ Jr., Akamine N, Knobel E (2008). Myocardial depression in sepsis. Shock 30 (Suppl. 1 14 17.
13. Fraser ID, Marston SB (1995). In vitro motility analysis of smooth muscle caldesmon control of actin-tropomyosin filament movement. J Biol Chem 270 : 19688 19693.
14. Geeves MA, Lehrer SS (1994). Dynamics of the muscle thin filament regulatory switch: the size of the cooperative unit. Biophys J 67 : 273 282.
15. Gordon AM, LaMadrid MA, Chen Y, Luo Z, Chase PB (1997). Calcium regulation of skeletal muscle thin filament motility in vitro. Biophys J 72 : 1295 1307. (Pubitemid 27113652)
16. Gupta A, Aberle NS 2nd., Ren J, Sharma AC (2005). Endothelin-converting enzyme-1-mediated signaling in adult rat ventricular myocyte contractility and apoptosis during sepsis. J Mol Cell Cardiol 38 : 527 537. (Pubitemid 40331943)
17. Hitchcock-DeGregori SE, Varnell TA (1990). Tropomyosin has discrete actin-binding sites with sevenfold and fourteen-fold periodicities. J Mol Biol 214 : 885 896. (Pubitemid 20263953)
18. Hotchkiss RS, Karl IE (2003). The pathophysiology and treatment of sepsis. N Engl J Med 348 : 138 150.
19. Krown KA, Page MT, Nguyen C, Zechner D, Gutierrez V, Comstock KL et al. (1996). Tumor necrosis factor α-induced apoptosis in cardiac myocytes. Involvement of the sphingolipid signaling cascade in cardiac cell death. J Clin Invest 98 : 2854 2865.
20. Lancel S, Joulin O, Favory R, Goossens JF, Kluza J, Chopin C et al. (2005). Ventricular myocyte caspases are directly responsible for endotoxin-induced cardiac dysfunction. Circulation 111 : 2596 2604.
21. Lazarides E (1976). Actin, alpha-actinin, and tropomyosin interaction in the structural organization of actin filaments in non muscle cells. J Cell Biol 68 : 202 219.
22. Lehrer SS, Geeves MA (1998). The muscle thin filament as a classical cooperative/allosteric regulatory system. J Mol Biol 277 : 1081 1089.
23. McLachlan AD, Stewart M (1976). The 14-fold periodicity in alpha-tropomyosin and the interaction with actin. J Mol Biol 103 : 271 298.
24. Maeder M, Fehr T, Rickli H, Ammann P (2006). Sepsis-associated myocardial dysfunction, diagnostic and prognostic impact of cardiac troponins and natriuretic peptides. Chest 129 : 1349 1366.
25. Mann DL, Kent RL, Parsons B, Cooper GIV (1992). Adrenergic effects on the biology of the adult mammalian cardiocyte. Circulation 85 : 790 804.
26. Miano JM, Ramanan N, Georger MA, de Mesy Bentley KL, Emerson RL, Balza RO et al. (2004). Restricted inactivation of serum response factor to the cardiovascular system. Proc Natl Acad Sci USA 101 : 17132 17137.
27. Nicholson DW, Ali A, Thornberry NA, Vaillancourt JP, Ding CK, Gallant M et al. (1995). Identification and inhibition of the ICE/CED-3 protease necessary for mammalian apoptosis. Nature 376 : 37 43.
28. Niu Z, Yu W, Zhang SX, Barron M, Belaguli NS, Schneider MD et al. (2005). Conditional mutagenesis of the murine serum response factor gene blocks cardiogenesis and the transcription of downstream gene targets. J Biol Chem 280 : 32531 32538.
29. Opie LH, Walpoth B, Barsacchi R (1985). Calcium and catecholamines: relevance to cardiomyopathies and significance in therapeutic strategies. J Mol Cell Cardiol 17 : 21 34.
30. Parlakian A, Tuil D, Hamard G, Tavernier G, Hentzen D, Concordet JP et al. (2004). Targeted inactivation of serum response factor in the developing heart results in myocardial defects and embryonic lethality. Mol Cell Biol 24 : 5281 5289.
31. Powers FM, Farias S, Minami H, Martin AF, Solaro RJ, Law WR (1998). Cardiac myofilament protein function is altered during sepsis. J Mol Cell Cardiol 30 : 967 978.
32. Ren J, Ren BH, Sharma AC (2002). Sepsis-induced depressed contractile function of isolated ventricular myocytes is due to altered calcium transient properties. Shock 18 : 285 288.
33. Rona G (1985). Catecholamine cardiotoxicity. J Mol Cell Cardiol 17 : 291 306.
34. Sharma AC (2007). Sepsis-induced myocardial dysfunction. Shock 28 : 265 269.
35. Thornberry NA, Lazebnik Y (1998). Caspases: enemies within. Science 281 : 1312 1316.
36. Tung RH, Garcia C, Morss AM, Pino RM, Fifer MA, Thompson BT et al. (2004). Utility of B-type natriuretic peptide for the evaluation of intensive care unit shock. Crit Care Med 32 : 1643 1647.
37. Weigt C, Wegner A, Koch MH (1991). Rate and mechanism of the assembly of tropomyosin with actin filaments. Biochemistry 30 : 10700 10707.