Time-course of spontaneous transformation of CD-1 mouse embryonic fibroblasts

Anticancer Research

Volumn 23, Issue 2 B, 2003, Pages 1373-1377

  Rossi, Ottavio ^a   Barbieri, Ottavia ^b   Frosina, Guido ^a  

^a UNIVERSITY OF GENOA   (Italy)

^b UNIVERSITY OF GENOA   (Italy)

Author keywords

DNA repair; Enbryonic fibroblasts; Immortalization; Oxidative damage

Indexed keywords

ANIMAL CELL; ANIMAL TISSUE; ARTICLE; BLAST TRANSFORMATION; CELL ACTIVITY; CELL CLONE; CELL DENSITY; CELL GROWTH; CELL LOSS; CELL PROLIFERATION; CELL PROTECTION; COLONY FORMING CELL; CONTROLLED STUDY; EMBRYO; EMBRYO CELL; FIBROBLAST; FIBROBLAST CULTURE; MOUSE; MOUSE STRAIN; NONHUMAN; PRIORITY JOURNAL;

ANIMALS; CELL ADHESION; CELL DIVISION; CELL LINE, TRANSFORMED; CELL TRANSFORMATION, NEOPLASTIC; CELLS, CULTURED; CLONE CELLS; COLONY-FORMING UNITS ASSAY; EMBRYO; FIBROBLASTS; MICE; MICE, INBRED STRAINS; TIME FACTORS;

EID: 0038238274     PISSN: 02507005     EISSN: None     Source Type: Journal    
DOI: None     Document Type: Article

References (16)

1. Frosina G: Counteracting spontaneous transformation via overexpression of rate-limiting DNA base excision repair enzymes. Carcinogenesis 22: 1335-1341, 2001.
2. Sanford KK and Evans VJ: A quest for the mechanism of "spontaneous" malignant transformation in culture with associated advances in culture technology. J Natl Cancer Inst 68: 895-913, 1982.
3. Wright WE and Shay JW: Telomere dynamics in cancer progression and prevention: fundamental differences in human and mouse telomere biology. Nature Med 6: 849-851, 2000.
4. Beckman KB and Ames B.N: Oxidative decay of DNA. J Biol Chem 272: 19633-19636, 1997.
5. Marnett LJ: Oxyradicals and DNA damage. Carcinogenesis 21: 361-370, 2000.
6. Obata M, Lee GH, Kanda H, Kitagawa T and Ogawa K: Loss of heterozygosity at loci on chromosome 4, a common genetic event during the spontaneous immortalization of mouse embryonic fibroblasts. Mol Carcinogenesis 19: 17-24, 1997.
7. Tolstonog GV, Shoeman RL, Traub U and Traub P: Role of the intermediate filament protein vimentin in delaying senescence and in the spontaneous immortalization of mouse embryo fibroblasts. DNA Cell Biol 20: 509-529, 2001.
8. Spector DL, Goldman RD and Leinwand LA: Cells. A Laboratory Manual. Volume 1: Culture and Biochemical Analysis of Cells. New York, Cold Spring Harbor Laboratory Press, Cold Spring Harbor, 1998, pp. 8.4-8.6.
9. Schaeffer WI and Friend K: Efficient detection of soft agar grown colonies using a tetrazolium salt. Cancer Letters 1: 259-262, 1976.
10. Rubin H: The role of selection in progressive neoplastic transformation. Adv Cancer Res 83: 159-207, 2001.
11. Sanford KK, Parshad R, Jones G, Handleman S, Garrison C and Price F: Role of photosensitization and oxygen in chromosome stability and "spontaneous" malignant transformation in culture. J Natl Cancer Inst 63: 1245-1255, 1979.
12. Parshad R, Sanford KK, Jones GM and Tarone RE: Fluorescent-light induced chromosome damage and its prevention in mouse cells in culture. Proc Natl Acad Sci USA 75: 1830-1833, 1978.
13. Harvey DM and Levine AJ: p53 alteration is a common event in the spontaneous immortalization of primary BALB/c murine embryo fibroblasts. Genes and Development 5: 2375-2385, 1991.
14. Noble JR, Rogan EM, Neumann AA, Maclean K, Bryan TM and Reddel RR: Association of extended in vitro proliferative potential with loss of p16INK4 expression. Oncogene 13: 1259-1268, 1996.
15. Hainaut P, Soussi T, Shomer B, Hollstein M, Greenblatt M, Hovig E, Harris CC and Montesano R: Database of p53 gene somatic mutations in human tumors and cell lines: updated compilation and future prospects. Nucleic Acids Res 25: 151-157, 1997.
16. Rubin H and Xu K: Evidence for the progressive and adaptive nature of spontaneous transformation in the NIH 3T3 cell line. Proc Natl Acad Sci USA 86: 1860-1864, 1989.