Cloning and functional characterization of pig CMP- N-acetylneuraminic acid hydroxylase for the synthesis of N-glycolylneuraminic acid as the xenoantigenic determinant in pig-human xenotransplantation

Biochemical Journal

Volumn 427, Issue 1, 2010, Pages 179-188

  Song, Kwon Ho ^a   Kang, Yun Jeong ^a   Jin, Un Ho ^a   Park, Yong Il ^b   Kim, Sung Min ^b   Seong, Hwan Hoo ^c   Hwang, Seongsoo ^c   Yang, Boh Suk ^c   Im, Gi Sun ^c   Min, Kwan Sik ^d,e   Kim, Jin Hoi ^d,e   Chang, Young Chae ^f   Kim, Nam Hyung ^g   Lee, Young Choon ^h   Kim, Cheorl Ho ^a  

^a Sungkyunkwan University   (South Korea)

^b The Catholic University of Korea   (South Korea)

^c National Institute of Animal Science   (South Korea)

^d Hankyong National University   (South Korea)

^e Konkuk University   (South Korea)

^f Catholic University of Daegu   (South Korea)

^g Chungbuk National University   (South Korea)

^h Dong A University   (South Korea)

Author keywords

CMP N acetylneuraminic acid hydroxylase; Hanganutziu Deicher; Human serum mediated cytotoxicity; Hyperacute rejection; N glycolylneuraminic acid

Indexed keywords

COLON TISSUES; ECTOPIC EXPRESSIONS; EXPRESSION PATTERNS; FUNCTIONAL CHARACTERIZATION; HANGANUTZIU-DEICHER; HYDROXYLASES; KEY ENZYMES; N-ACETYLNEURAMINIC ACID; N-GLYCOLYLNEURAMINIC ACID; OPEN READING FRAME; PIG KIDNEY; SHORT HAIRPIN RNA; SMALL INTESTINE; TRANSFECTANTS; TRANSFECTED CELLS; XENOTRANSPLANTATION;

AMINO ACIDS; BODY FLUIDS; CLONING; CYTOTOXICITY; ORGANIC ACIDS; RNA;

ENDOTHELIAL CELLS;

AMINO ACID; CYTIDINE PHOSPHATE N ACYLNEURAMINATE PHOSPHODIESTERASE; IMMUNOGLOBULIN M; MESSENGER RNA; N GLYCOLOYLNEURAMINIC ACID; SHORT HAIRPIN RNA; CMPACETYLNEURAMINATE MONOOXYGENASE; HETEROPHILE ANTIGEN; LACTATE DEHYDROGENASE; MIXED FUNCTION OXIDASE; N-GLYCOLYLNEURAMINIC ACID; NEURAMINIC ACID DERIVATIVE; SMALL INTERFERING RNA;

ANIMAL EXPERIMENT; ANIMAL TISSUE; ANTIGENICITY; ARTICLE; CARBOHYDRATE SYNTHESIS; COLON; CONTROLLED STUDY; CYTOTOXICITY; ENZYME SYNTHESIS; GENE EXPRESSION; GENE VECTOR; GENETIC TRANSFECTION; GRAFT REJECTION; HUMAN; HUMAN CELL; MOLECULAR CLONING; NONHUMAN; NUCLEOTIDE SEQUENCE; OPEN READING FRAME; PRIORITY JOURNAL; PROTEIN FUNCTION; RECTUM; SPLEEN; SWINE; TONGUE; XENOTRANSPLANTATION; AMINO ACID SEQUENCE; ANIMAL; CELL CULTURE; CYTOLOGY; DRUG ANTAGONISM; ENZYMOLOGY; GENETICS; HIGH PERFORMANCE LIQUID CHROMATOGRAPHY; KIDNEY; METABOLISM; MOLECULAR GENETICS; REVERSE TRANSCRIPTION POLYMERASE CHAIN REACTION; SEQUENCE HOMOLOGY; SMALL INTESTINE; VASCULAR ENDOTHELIUM; WESTERN BLOTTING; XENOGRAFT;

SUIDAE;

AMINO ACID SEQUENCE; ANIMALS; ANTIGENS, HETEROPHILE; BASE SEQUENCE; BLOTTING, WESTERN; CELLS, CULTURED; CHROMATOGRAPHY, HIGH PRESSURE LIQUID; CLONING, MOLECULAR; ENDOTHELIUM, VASCULAR; HUMANS; INTESTINE, SMALL; KIDNEY; L-LACTATE DEHYDROGENASE; MIXED FUNCTION OXYGENASES; MOLECULAR SEQUENCE DATA; NEURAMINIC ACIDS; REVERSE TRANSCRIPTASE POLYMERASE CHAIN REACTION; RNA, MESSENGER; RNA, SMALL INTERFERING; SEQUENCE HOMOLOGY, AMINO ACID; SEQUENCE HOMOLOGY, NUCLEIC ACID; SWINE; TRANSPLANTATION, HETEROLOGOUS;

EID: 77950874526     PISSN: 02646021     EISSN: 14708728     Source Type: Journal    
DOI: 10.1042/BJ20090835     Document Type: Article

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