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Volumn 10, Issue 2, 2010, Pages 130-138

Characterization and improvement of cell line performance via flow cytometry and cell sorting

Author keywords

Cell surface affinity matrix; FACS; Hybridoma; Productivity; Protein microarray

Indexed keywords

AFFINITY MATRIX; BIOPROCESSES; CELL LINES; CELL SORTING; CELL SURFACE AFFINITY MATRIX; CELL SURFACES; CRITICAL STEPS; FLOW-CYTOMETRIC CELL SORTING; HIGH-THROUGHPUT METHOD; HYBRIDOMA CELLS; INITIAL POPULATION; LOW-COST STRATEGIES; MAMMALIAN CELLS; PRODUCTIVITY IMPROVEMENTS; PROTEIN MICROARRAY; SPECIFIC PRODUCTIVITY; STAINING METHOD;

EID: 77950837308     PISSN: 16180240     EISSN: 16182863     Source Type: Journal    
DOI: 10.1002/elsc.200900076     Document Type: Article
Times cited : (6)

References (42)
  • 1
    • 33644846608 scopus 로고    scopus 로고
    • Biotech drug market steadily expands
    • S. Lawrence, Biotech drug market steadily expands, Nat. Biotechnol. 2005, 23, 1466-1466
    • (2005) Nat. Biotechnol. , vol.23 , pp. 1466-1466
    • Lawrence, S.1
  • 2
    • 33746163862 scopus 로고    scopus 로고
    • Biopharmaceutical benchmarks 2006
    • G. Walsh, Biopharmaceutical benchmarks 2006, Nat. Biotechnol. 2006, 24, 769-776.
    • (2006) Nat. Biotechnol. , vol.24 , pp. 769-776
    • Walsh, G.1
  • 3
    • 8344271026 scopus 로고    scopus 로고
    • Production of recombinant protein therapeutics in cultivated mammalian cells
    • F. M. Wurm, Production of recombinant protein therapeutics in cultivated mammalian cells, Nat. Biotechnol. 2004, 22, 1393-1398.
    • (2004) Nat. Biotechnol. , vol.22 , pp. 1393-1398
    • Wurm, F.M.1
  • 4
    • 23844433927 scopus 로고    scopus 로고
    • Animal cell cultures: Recent achievements and perspectives in the production of biopharmaceuticals
    • M. Butler, Animal cell cultures: Recent achievements and perspectives in the production of biopharmaceuticals, Appl. Microbiol. Biotechnol. 2005, 68, 283-291.
    • (2005) Appl. Microbiol. Biotechnol. , vol.68 , pp. 283-291
    • Butler, M.1
  • 5
    • 1642325279 scopus 로고    scopus 로고
    • Life and death in mammalian cell culture: Strategies for apoptosis inhibition
    • N. Arden, M. J. Betenbaugh, Life and death in mammalian cell culture: Strategies for apoptosis inhibition, Trends Biotechnol. 2004, 22, 174-180.
    • (2004) Trends Biotechnol. , vol.22 , pp. 174-180
    • Arden, N.1    Betenbaugh, M.J.2
  • 6
    • 0028765762 scopus 로고
    • Induction of apoptosis in nutrient-deprived cultures of hybridoma and myeloma cells
    • S. Mercille, B. Massie, Induction of apoptosis in nutrient-deprived cultures of hybridoma and myeloma cells, Biotechnol. Bioeng. 1994, 44, 1140-1154.
    • (1994) Biotechnol. Bioeng. , vol.44 , pp. 1140-1154
    • Mercille, S.1    Massie, B.2
  • 7
    • 0032486578 scopus 로고    scopus 로고
    • In hybridoma cultures, deprivation of any single amino acid leads to apoptotic death, which is suppressed by the expression of the bcl-2 gene
    • N. H. Simpson, R. P. Singh, A. Perani, C. Goldenzon, M. Al-Rubeai, In hybridoma cultures, deprivation of any single amino acid leads to apoptotic death, which is suppressed by the expression of the bcl-2 gene, Biotechnol. Bioeng. 1998, 59, 90-98.
    • (1998) Biotechnol. Bioeng. , vol.59 , pp. 90-98
    • Simpson, N.H.1    Singh, R.P.2    Perani, A.3    Goldenzon, C.4    Al-Rubeai, M.5
  • 9
    • 0037161615 scopus 로고    scopus 로고
    • The protection of hybridoma cells from apoptosis by caspase inhibition allows culture recovery when exposed to non-inducing conditions
    • A. Tinto, C. Gabernet, J. Vives, E. Prats et al., The protection of hybridoma cells from apoptosis by caspase inhibition allows culture recovery when exposed to non-inducing conditions, J. Biotechnol. 2002, 95, 205-214.
    • (2002) J. Biotechnol. , vol.95 , pp. 205-214
    • Tinto, A.1    Gabernet, C.2    Vives, J.3    Prats, E.4
  • 11
    • 24344498420 scopus 로고    scopus 로고
    • On-line monitoring and control of substrate concentrations in biological processes by flow injection analysis systems
    • I. L. Rhee, A. Ritzka, T. Scheper, On-line monitoring and control of substrate concentrations in biological processes by flow injection analysis systems, Biotechnol. Bioprocess Eng. 2004, 9, 156-165.
    • (2004) Biotechnol. Bioprocess Eng. , vol.9 , pp. 156-165
    • Rhee, I.L.1    Ritzka, A.2    Scheper, T.3
  • 12
    • 0032724272 scopus 로고    scopus 로고
    • Bioanalytics: Detailed insight into bioprocesses
    • T. Scheper, B. Hitzmann, E. Stark, R. Ulber et al., Bioanalytics: detailed insight into bioprocesses, Anal. Chim. Acta 1999, 400, 121-134.
    • (1999) Anal. Chim. Acta , vol.400 , pp. 121-134
    • Scheper, T.1    Hitzmann, B.2    Stark, E.3    Ulber, R.4
  • 14
    • 35548990601 scopus 로고    scopus 로고
    • Selection methods for high-producing mammalian cell lines
    • DOI 10.1016/j.tibtech.2007.07.002, PII S0167779907001825
    • S. M. Browne, M. Al-Rubeai, Selection methods for high-producing mammalian cell lines, Trends Biotechnol. 2007, 25, 425-432. (Pubitemid 350147111)
    • (2007) Trends in Biotechnology , vol.25 , Issue.9 , pp. 425-432
    • Browne, S.M.1    Al-Rubeai, M.2
  • 16
    • 0033969002 scopus 로고    scopus 로고
    • Green fluorescent protein as a second selectable marker for selection of high producing clones from transfected CHO cells
    • Y. G. Meng, J. Liang, W. L. Wong, V. Chisholm, Green fluorescent protein as a second selectable marker for selection of high producing clones from transfected CHO cells, Gene 2000, 242, 201-207.
    • (2000) Gene , vol.242 , pp. 201-207
    • Meng, Y.G.1    Liang, J.2    Wong, W.L.3    Chisholm, V.4
  • 17
    • 34247280567 scopus 로고    scopus 로고
    • Accelerated clone selection for recombinant CHO cells using a FACS-based high-throughput screen
    • C. T. DeMaria, V. Cairns, C. Schwarz, J. Zhang et al., Accelerated clone selection for recombinant CHO cells using a FACS-based high-throughput screen, Biotechnol. Prog. 2007, 23, 465-472.
    • (2007) Biotechnol. Prog. , vol.23 , pp. 465-472
    • DeMaria, C.T.1    Cairns, V.2    Schwarz, C.3    Zhang, J.4
  • 18
    • 0025332641 scopus 로고
    • Selective cloning of hybridoma cells for enhanced immunoglobulin production using flow cytometric cell sorting and automated laser nephelometry
    • P. Marder, R. S. Maciak, R. L. Fouts, R. S. Baker, J. J. Starling, Selective cloning of hybridoma cells for enhanced immunoglobulin production using flow cytometric cell sorting and automated laser nephelometry, Cytometry 1990, 11, 498-505.
    • (1990) Cytometry , vol.11 , pp. 498-505
    • Marder, P.1    Maciak, R.S.2    Fouts, R.L.3    Baker, R.S.4    Starling, J.J.5
  • 19
    • 0028430190 scopus 로고
    • Stability of producer hybridoma cell lines after cell sorting: A case study
    • S. J. Kromenaker, F. Srienc, Stability of producer hybridoma cell lines after cell sorting: a case study, Biotechnol. Prog. 1994, 10, 299-307.
    • (1994) Biotechnol. Prog. , vol.10 , pp. 299-307
    • Kromenaker, S.J.1    Srienc, F.2
  • 20
    • 0025472073 scopus 로고
    • Flow cytometric study of hybridoma cell culture: Correlation between cell surface fluorescence and IgG production rate
    • S. Sen, W. S. Hu, F. Srienc, Flow cytometric study of hybridoma cell culture: Correlation between cell surface fluorescence and IgG production rate, Enzyme Microb. Technol. 1990, 12, 571-576.
    • (1990) Enzyme Microb. Technol. , vol.12 , pp. 571-576
    • Sen, S.1    Hu, W.S.2    Srienc, F.3
  • 22
    • 0034446598 scopus 로고    scopus 로고
    • Efficient selection of high-producing subclones during gene amplification of recombinant Chinese hamster ovary cells by flow cytometry and cell sorting
    • N. Borth, M. Zeyda, R. Kunert, H. Katinger, Efficient selection of high-producing subclones during gene amplification of recombinant Chinese hamster ovary cells by flow cytometry and cell sorting, Biotechnol. Bioeng. 2000, 71, 266-273.
    • (2000) Biotechnol. Bioeng. , vol.71 , pp. 266-273
    • Borth, N.1    Zeyda, M.2    Kunert, R.3    Katinger, H.4
  • 23
    • 0028960201 scopus 로고
    • Analysis and sorting of live cells according to secreted molecules, relocated to a cell-surface affinity matrix
    • R. Manz, M. Assenmacher, E. Pfluger, S. Miltenyi, A. Radbruch, Analysis and sorting of live cells according to secreted molecules, relocated to a cell-surface affinity matrix, Proc. Natl. Acad. Sci. USA 1995, 92, 1921-1925.
    • (1995) Proc. Natl. Acad. Sci. USA , vol.92 , pp. 1921-1925
    • Manz, R.1    Assenmacher, M.2    Pfluger, E.3    Miltenyi, S.4    Radbruch, A.5
  • 24
    • 0025217465 scopus 로고
    • Gel microdroplets and flow cytometry: Rapid determination of antibody secretion by individual cells within a cell population
    • DOI 10.1038/nbt0490-333
    • K. T. Powell, J. C. Weaver, Gel microdroplets and flow cytometry: rapid determination of antibody secretion by individual cells within a cell population, Biotechnology 1990, 8, 333-337. (Pubitemid 20138303)
    • (1990) Bio/Technology , vol.8 , Issue.4 , pp. 333-337
    • Powell, K.T.1    Weaver, J.C.2
  • 25
  • 26
    • 0025700415 scopus 로고
    • The loss of antibody productivity in continuous culture of hybridoma cells
    • K. K. Frame, W. S. Hu, The loss of antibody productivity in continuous culture of hybridoma cells, Biotechnol. Bioeng. 1990, 35, 469-476.
    • (1990) Biotechnol. Bioeng. , vol.35 , pp. 469-476
    • Frame, K.K.1    Hu, W.S.2
  • 27
    • 0022352450 scopus 로고
    • A quantitative stability analysis of human monoclonal-antibody production by heteromyeloma hybridomas, using an immunofluorescent technique
    • S. Gardner, A. L. H. Chiu, N. E. Maki, J. F. Harris, A quantitative stability analysis of human monoclonal-antibody production by heteromyeloma hybridomas, using an immunofluorescent technique, J. Immunol. Methods 1985, 85, 335-346.
    • (1985) J. Immunol. Methods , vol.85 , pp. 335-346
    • Gardner, S.1    Chiu, A.L.H.2    Maki, N.E.3    Harris, J.F.4
  • 28
    • 0027910075 scopus 로고
    • Stability of antibody productivity is improved when hybridoma cells are entrapped in calcium alginate beads
    • G. M. Lee, B. O. Palsson, Stability of antibody productivity is improved when hybridoma cells are entrapped in calcium alginate beads, Biotechnol. Bioeng. 1993, 42, 1131-1135.
    • (1993) Biotechnol. Bioeng. , vol.42 , pp. 1131-1135
    • Lee, G.M.1    Palsson, B.O.2
  • 30
    • 0028107384 scopus 로고
    • Perfusible and non-perfusible supports with monoclonal-antibodies for bioaffinity chromatography of Escherichia-coli penicillin amidase within its pH stability range
    • V. Kasche, N. Gottschlich, A. Lindberg, C. Niebuhrredder, J. Schmieding, Perfusible and non-perfusible supports with monoclonal-antibodies for bioaffinity chromatography of Escherichia-coli penicillin amidase within its pH stability range, J. Chromatogr. A 1994, 660, 137-145.
    • (1994) J. Chromatogr. A , vol.660 , pp. 137-145
    • Kasche, V.1    Gottschlich, N.2    Lindberg, A.3    Niebuhrredder, C.4    Schmieding, J.5
  • 31
    • 0013261473 scopus 로고    scopus 로고
    • Establishment of a human somatic hybrid cell line for recombinant protein production
    • M. S. Cho, H. Yee, S. Chan, Establishment of a human somatic hybrid cell line for recombinant protein production, J. Biomed. Sci. 2002, 9, 631-638.
    • (2002) J. Biomed. Sci. , vol.9 , pp. 631-638
    • Cho, M.S.1    Yee, H.2    Chan, S.3
  • 33
    • 37149032508 scopus 로고    scopus 로고
    • Optimization of a microarray sandwich-ELISA against hINF-gamma on a modified nitrocellulose membrane
    • M. Reck, F. Stahl, J. G. Walter, M. Hollas, D. Melzner, T. Scheper, Optimization of a microarray sandwich-ELISA against hINF-gamma on a modified nitrocellulose membrane, Biotechnol. Prog. 2007, 23, 1498-1505.
    • (2007) Biotechnol. Prog. , vol.23 , pp. 1498-1505
    • Reck, M.1    Stahl, F.2    Walter, J.G.3    Hollas, M.4    Melzner, D.5    Scheper, T.6
  • 34
    • 0025756428 scopus 로고
    • Nucleosomes occurring in protein-free hybridoma cell culture. Evidence for programmed cell death
    • F. Franek, J. Dolnikova, Nucleosomes occurring in protein-free hybridoma cell culture. Evidence for programmed cell death, FEBS Lett. 1991, 284, 285-287.
    • (1991) FEBS Lett. , vol.284 , pp. 285-287
    • Franek, F.1    Dolnikova, J.2
  • 35
    • 0026254349 scopus 로고
    • Growth, metabolic, and antibody production kinetics of hybridoma cell culture. 2. Effects of serum concentration, dissolved oxygen concentration, and medium pH in a batch reactor
    • S. S. Ozturk, B. O. Palsson, Growth, metabolic, and antibody production kinetics of hybridoma cell culture. 2. Effects of serum concentration, dissolved oxygen concentration, and medium pH in a batch reactor, Biotechnol. Prog. 1991, 7, 481-494.
    • (1991) Biotechnol. Prog. , vol.7 , pp. 481-494
    • Ozturk, S.S.1    Palsson, B.O.2
  • 36
    • 0027024341 scopus 로고
    • Specific monoclonal antibody productivity and the cell cycle-comparisons of batch, continuous and perfusion cultures
    • M. Al-Rubeai, A. N. Emery, S. Chalder, D. C. Jan, Specific monoclonal antibody productivity and the cell cycle-comparisons of batch, continuous and perfusion cultures, Cytotechnology 1992, 9, 85-97.
    • (1992) Cytotechnology , vol.9 , pp. 85-97
    • Al-Rubeai, M.1    Emery, A.N.2    Chalder, S.3    Jan, D.C.4
  • 37
    • 0026878379 scopus 로고
    • Relationship between hybridoma growth and monoclonal antibody production
    • P. M. Hayter, N. F. Kirkby, R. E. Spier, Relationship between hybridoma growth and monoclonal antibody production, Enzyme Microb. Technol. 1992, 14, 454-461.
    • (1992) Enzyme Microb. Technol. , vol.14 , pp. 454-461
    • Hayter, P.M.1    Kirkby, N.F.2    Spier, R.E.3
  • 39
    • 0026467684 scopus 로고
    • The potential of flow cytometric analysis for the characterization of hybridoma cells in suspension cultures
    • J. M. Coco-Martin, J. W. Oberink, T. A. van der Velden-de Groot, E. C. Beuvery, The potential of flow cytometric analysis for the characterization of hybridoma cells in suspension cultures, Cytotechnology 1992, 8, 65-74.
    • (1992) Cytotechnology , vol.8 , pp. 65-74
    • Coco-Martin, J.M.1    Oberink, J.W.2    Van Der Velden-de Groot, T.A.3    Beuvery, E.C.4
  • 40
    • 0026219288 scopus 로고
    • Cell-cycle-dependent protein accumulation by producer and nonproducer murine hybridoma cell-lines-a population analysis
    • S. J. Kromenaker, F. Srienc, Cell-cycle-dependent protein accumulation by producer and nonproducer murine hybridoma cell-lines-a population analysis, Biotechnol. Bioeng. 1991, 38, 665-677.
    • (1991) Biotechnol. Bioeng. , vol.38 , pp. 665-677
    • Kromenaker, S.J.1    Srienc, F.2
  • 41
    • 0028029024 scopus 로고
    • Cell cycle kinetics of the accumulation of heavy and light chain immunoglobulin proteins in a mouse hybridoma cell line
    • S. J. Kromenaker, F. Srienc, Cell cycle kinetics of the accumulation of heavy and light chain immunoglobulin proteins in a mouse hybridoma cell line, Cytotechnology 1994, 14, 205-218.
    • (1994) Cytotechnology , vol.14 , pp. 205-218
    • Kromenaker, S.J.1    Srienc, F.2
  • 42
    • 0028231367 scopus 로고
    • Effect of lactic acid on the kinetics of growth and antibody production in a murine hybridoma: Secretion patterns during the cell cycle
    • S. J. Kromenaker, F. Srienc, Effect of lactic acid on the kinetics of growth and antibody production in a murine hybridoma: Secretion patterns during the cell cycle, J. Biotechnol. 1994, 34, 13-34.
    • (1994) J. Biotechnol. , vol.34 , pp. 13-34
    • Kromenaker, S.J.1    Srienc, F.2


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