The utility and application of real-time PCR and FISH in the detection of single-copy gene targets in Escherichia coli O157:H7 and Salmonella Typhimurium

Canadian Journal of Microbiology

Volumn 56, Issue 3, 2010, Pages 254-262

  Haffar, Merriam ^a   Gilbride, Kimberley A ^a  

^a RYERSON UNIVERSITY   (Canada)

Author keywords

Escherichia coli O157:H7; FISH; qPCR; Salmonella; Single copy gene; Wastewater; Waterborne pathogens

Indexed keywords

DETECTION LIMITS; ENVIRONMENTAL SAMPLE; ESCHERICHIA COLI O157:H7; FLUORESCENT IN SITU HYBRIDIZATION; FLUORESCENT SIGNALS; INHIBITORY EFFECT; INNER MEMBRANES; INTIMIN; PATHOGEN DETECTION; PROTEIN RECEPTORS; REAL-TIME PCR; REAL-TIME PCR (QPCR); SALMONELLA ENTERICA SEROVAR TYPHIMURIUM; SALMONELLA TYPHIMURIUM; SIGNAL AMPLIFICATIONS; SINGLE-COPY GENES; TARGET CELLS; VIRULENCE GENE; WATER-BORNE PATHOGENS;

AMPLIFICATION; ASSAYS; DNA; DNA SEQUENCES; ESCHERICHIA COLI; FISH; FLUORESCENCE; GENES; GENETIC ENGINEERING; MEATS; POLYMERASE CHAIN REACTION; PROBES; PROTEINS; SALMONELLA; SIGNAL DETECTION; SIGNAL PROCESSING; TARGETS; TRACE ANALYSIS; WASTEWATER;

PATHOGENS;

BACTERIAL PROTEIN; INNER MEMBRANE INVASIVE PROTEIN; INTIMIN PROTEIN RECEPTOR; UNCLASSIFIED DRUG;

BACTERIUM; BIOASSAY; BIOENGINEERING; GENE; HYBRIDIZATION; IMMUNOASSAY; POLYMERASE CHAIN REACTION; REAL TIME; VIRULENCE; WASTEWATER;

ARTICLE; BACTERIUM IDENTIFICATION; CAMPYLOBACTER JEJUNI; CONTROLLED STUDY; ESCHERICHIA COLI O157; FLUORESCENCE IN SITU HYBRIDIZATION; GENE AMPLIFICATION; GENE NUMBER; KLEBSIELLA PNEUMONIAE; MOLECULAR PROBE; NONHUMAN; PRIORITY JOURNAL; QUANTITATIVE ANALYSIS; REAL TIME POLYMERASE CHAIN REACTION; SALMONELLA TYPHIMURIUM; SENSITIVITY AND SPECIFICITY; SHIGELLA FLEXNERI; STAPHYLOCOCCUS AUREUS; TARGET CELL; WASTE WATER;

DNA PRIMERS; ESCHERICHIA COLI O157; IN SITU HYBRIDIZATION, FLUORESCENCE; POLYMERASE CHAIN REACTION; SALMONELLA TYPHIMURIUM; SENSITIVITY AND SPECIFICITY; WASTE DISPOSAL, FLUID; WATER MICROBIOLOGY;

ESCHERICHIA COLI; SALMONELLA; SALMONELLA ENTERICA SUBSP. ENTERICA SEROVAR TYPHIMURIUM; SALMONELLA TYPHIMURIUM;

EID: 77950683792     PISSN: 00084166     EISSN: None     Source Type: Journal    
DOI: 10.1139/W09-126     Document Type: Article

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