A filtration-based real-time PCR method for the quantitative detection of viable Salmonella enterica and Listeria monocytogenes in food samples

Food Microbiology

Volumn 26, Issue 3, 2009, Pages 311-316

  D'Urso, Oscar Fernando ^a,b   Poltronieri, Palmiro ^c   Marsigliante, Santo ^a   Storelli, Carlo ^a   Hernández, Marta ^b   Rodríguez Lázaro, David ^b  

^a UNIVERSITY OF SALENTO   (Italy)

^b INSTITUTO TECNOLÓGICO AGRARIO DE CASTILLA Y LEÓN   (Spain)

^c CNR   (Italy)

Author keywords

Filtration; Foodborne pathogens; Listeria; Molecular detection methods; Real time PCR; Salmonella; Viable detection

Indexed keywords

BACTERIAL DNA; YOGHURT;

ARTICLE; BACTERIAL COUNT; CHEMISTRY; FILTRATION; FOOD CONTAMINATION; FOOD CONTROL; GENETICS; ISOLATION AND PURIFICATION; LISTERIA MONOCYTOGENES; METHODOLOGY; MICROBIOLOGY; POLYMERASE CHAIN REACTION; REPRODUCIBILITY; SALMONELLA ENTERICA; SENSITIVITY AND SPECIFICITY; SPECIES DIFFERENCE; STANDARD;

COLONY COUNT, MICROBIAL; DNA, BACTERIAL; FILTRATION; FOOD CONTAMINATION; FOOD MICROBIOLOGY; LISTERIA MONOCYTOGENES; POLYMERASE CHAIN REACTION; REPRODUCIBILITY OF RESULTS; SALMONELLA ENTERICA; SENSITIVITY AND SPECIFICITY; SPECIES SPECIFICITY; YOGURT;

BACTERIA (MICROORGANISMS); LISTERIA; LISTERIA MONOCYTOGENES; SALMONELLA; SALMONELLA ENTERICA;

EID: 61449145431     PISSN: 07400020     EISSN: 10959998     Source Type: Journal    
DOI: 10.1016/j.fm.2008.12.006     Document Type: Article

References (24)

1. Allmann M., Hofelein C., Koppel E., Luthy J., Meyer R., Niederhauser C., Wegmuller B., and Candrian U. Polymerase chain reaction (PCR) for detection of pathogenic microorganisms in bacteriological monitoring of dairy products. Res. Microbiol. 146 (1995) 85-97
2. Anonymous. Microbiology of Food and Animal Feeding Stuffs - Horizontal Method for the Detection and Enumeration of Listeria monocytogenes - Part 2: Enumeration Method. ISO 11290-2: 1998 (1998), International Organization for Standardization, Geneva, Switzerland
3. Anonymous. Microbiology of Food and Animal Feeding Stuffs-Protocol for the Validation of Alternative Method ISO/DIS 16140 (2003), International Organization for Standardization, Geneva, Switzerland
4. Del Mar Lleo M., Pierobon S., Tafi M.C., Signoretto C., and Canepari P. mRNA detection by reverse transcription-PCR for monitoring viability over time in an Enterococcus faecalis viable but nonculturable population maintained in a laboratory microcosm. Appl. Environ. Microbiol. 66 (2000) 4564-4567
5. Ingle C.A., and Kushner S.R. Development of an in vitro mRNA decay system for Escherichia coli: poly(A) polymerase I is necessary to trigger degradation. Proc. Natl. Acad. Sci. U.S.A. 93 (1996) 12926-12931
6. Jofré A., Martin B., Garriga M., Hugas M., Pla M., Rodriguez-Lazaro D., and Aymerich T. Simultaneous detection of Listeria monocytogenes and Salmonella by multiplex PCR in cooked ham. Food Microbiol. 22 (2005) 109-115
7. Knutsson R., Lofstrom C., Grage H., Hoorfar J., and Radstrom P. Modeling of 5′ nuclease real-time responses for optimization of a high-throughput enrichment PCR procedure for Salmonella enterica. J. Clin. Microbiol. 40 (2002) 52-60
8. López-Enríquez L., Rodríguez-Lázaro D., and Hernández M. Quantitative detection of Clostridium tyrobutyricum in milk by real-time PCR. Appl. Environ. Microbiol. 73 (2007) 3747-3751
9. Malorny B., Tassios P.T., Rådstrom P., Cook N., Wagner M., and Hoorfar J. Standardization of diagnostic PCR for the detection of foodborne pathogens. Int. J. Food Microbiol. 83 (2003) 39-48
10. Nocker A., and Camper A.K. Selective removal of DNA from dead cells of mixed bacterial communities by use of ethidium monoazide. Appl. Environ. Microbiol. 72 (2006) 1997-2004
11. Nogva H.K., Dromtorp S.M., Nissen H., and Rudi K. Ethidium monoazide for DNA-based differentiation of viable and dead bacteria by 5′-nuclease PCR. Biotechniques 34 (2003) 804-813
12. Rodríguez-Lázaro D., Hernández M., Scortti M., Esteve T., Vázquez-Boland J.A., and Pla M. Quantitative detection of Listeria monocytogenes and Listeria innocua by real-time PCR: assessment of hly, iap, and lin02483 targets and AmpliFluor technology. Appl. Environ. Microbiol. 70 (2004) 1366-1377
13. Rodríguez-Lázaro D., Jofré A., Aymerich T., Hugas M., and Pla M. Rapid quantitative detection of Listeria monocytogenes in meat products by real-time PCR. Appl. Environ. Microbiol. 70 (2004) 6299-6301
14. Rodríguez-Lázaro D., D'Agostino M., Pla M., and Cook N. A construction strategy for an internal amplification control (IAC) for molecular beacon-based real-time nucleic acid sequence-based amplification (NASBA). J. Clin. Microbiol. 42 (2004) 5832-5836
15. Rodríguez-Lázaro D., D'Agostino M., Herrewegh A., Pla M., Cook N., and Ikonomopoulos J. Real-time PCR-based methods for quantitative detection of Mycobacterium avium subsp. paratuberculosis in water and milk. Int. J. Food Microbiol. 101 (2005) 93-104
16. Rodríguez-Lázaro D., Jofré A., Aymerich T., Garriga M., and Pla M. Rapid quantitative detection of Listeria monocytogenes in salmon products: evaluation of pre-real-time PCR strategies. J. Food Prot. 68 (2005) 1467-1471
17. Rodríguez-Lázaro D., Pla M., Scortti M., Monzó H.J., and Vázquez-Boland J.A. A novel real-time PCR for Listeria monocytogenes that monitors analytical performance via an internal amplification control. Appl. Environ. Microbiol. 71 (2005) 9008-9012
18. Rodríguez-Lázaro D., Lewis D.A., Ocampo-Sosa A.A., Fogarty U., Makrai L., Navas J., Scortti M., Hernández M., and Vázquez-Boland J.A. An internally controlled real-time PCR method for quantitative species-specific detection and vapA genotyping of Rhodococcus equi. Appl. Environ. Microbiol. 72 (2006) 4256-4263
19. Rodríguez-Lázaro D., Hernández M., D'Agostino M., and Cook N. Application of nucleic acid sequence based amplification (NASBA) for the detection of viable foodborne pathogens: progress and challenges. J. Rapid Methods Autom. Microbiol. 14 (2006) 218-236
20. Rodríguez-Lázaro D., Lombard B., Smith H., Rzezutka A., D'Agostino M., Helmuth R., Schroeter A., Malorny B., Miko A., Guerra B., Davison J., Kobilinsky A., Hernández M., Bertheau Y., and Cook N. Trends in analytical methodology in food safety and quality: monitoring microorganisms and genetically modified organisms. Trends Food Sci. Technol. 18 (2007) 306-319
21. Rudi K., Naterstad K., Dromtorp S.M., and Holo H. Detection of viable and dead Listeria monocytogenes on gouda-like cheeses by real-time PCR. Lett. Appl. Microbiol. 40 (2005) 301-306
22. Rueckert A., Ronimus R.S., and Morgan H.W. Rapid differentiation and enumeration of the total, viable vegetative cell and spore content of thermophilic bacilli in milk powders with reference to Anoxybacillus flavithermus. J. Appl. Microbiol. 99 (2005) 1246-1255
23. Sheridan G.E.C., Masters C.I., Shallcross J.A., and Mackey B.M. Detection of mRNA by reverse transcription-PCR as an indicator of viability in Escherichia coli cells. Appl. Environ. Microbiol. 64 (1998) 1313-1318
24. Simon M.C., Gray D.I., and Cook N. DNA extraction and PCR methods for the detection of Listeria monocytogenes in cold-smoked salmon. Appl. Environ. Microbiol. 62 (1996) 822-824