Inhibition of orientia tsutsugamushi infection by a truncated recombinant 56-kDa outer membrane protein

Letters in Applied Microbiology

Volumn 50, Issue 5, 2010, Pages 445-451

  Park, S ^a   Hwang, K J ^a   Chu, H ^a   Park, S H ^a   Shim, S K ^a   Choi, Y S ^a   Kim, J S ^a   Park, M Y ^a  

^a Korea National Institute of Health   (South Korea)

Author keywords

Orientia tsutsugamushi infection; Outer membrane protein; Recombinant 56 kDa protein

Indexed keywords

ADHESION; AMINO ACIDS; BIOLOGICAL MEMBRANES; CELL ADHESION; CELLS; ESCHERICHIA COLI; ORGANIC ACIDS; TARGETS;

AMINO ACID RESIDUES; ANTIBODY ASSAYS; CELL-ADHESION ASSAYS; HELA CELL; IN-VITRO; INDIRECT IMMUNOFLUORESCENCE; ORIENTIA TSUTSUGAMUSHI INFECTION; OUTER MEMBRANE PROTEIN; POTENTIAL INHIBITORS; TARGET CELLS;

PROTEINS;

ANTIINFECTIVE AGENT; OUTER MEMBRANE PROTEIN; RECOMBINANT 56KDA OUTER MEMBRANE PROTEIN; UNCLASSIFIED DRUG; HYBRID PROTEIN; PROTEIN BINDING;

BACTERIAL DISEASE; COLIFORM BACTERIUM; INHIBITION; PROTEIN; RECOMBINATION;

ARTICLE; BACTERIAL INFECTION; BACTERIAL STRAIN; BACTERIUM ADHERENCE; CONTROLLED STUDY; DRUG SCREENING; ESCHERICHIA COLI; HELA CELL; IMMUNOFLUORESCENCE; NONHUMAN; NUCLEOTIDE SEQUENCE; ORIENTIA TSUTSUGAMUSHI; PROTEIN EXPRESSION; SCRUB TYPHUS; TARGET CELL; ANIMAL; CELL ADHESION; DOWN REGULATION; GENETICS; HELA CELL LINE; HUMAN; METABOLISM; MICROBIOLOGY; MOUSE; PATHOPHYSIOLOGY; PHYSIOLOGY;

ESCHERICHIA COLI; ORIENTIA TSUTSUGAMUSHI;

ANIMALS; BACTERIAL OUTER MEMBRANE PROTEINS; CELL ADHESION; DOWN-REGULATION; HELA CELLS; HUMANS; MICE; ORIENTIA TSUTSUGAMUSHI; PROTEIN BINDING; RECOMBINANT FUSION PROTEINS; SCRUB TYPHUS;

EID: 77950496681     PISSN: 02668254     EISSN: 1472765X     Source Type: Journal    
DOI: 10.1111/j.1472-765X.2010.02814.x     Document Type: Article

References (21)

1. Chao, C.C., Zhang, Z., Wang, H., Alkhalil, A. Ching, W.M. (2008) Serological reactivity and biochemical characterization of methylated and unmethylated forms of a recombinant protein fragment derived from outer membrane protein B of Rickettsia typhi. Clin Vaccine Immunol 4, 684 690. (Pubitemid 351967053)
2. Chattopadhyay, S. Richards, A.L. (2007) Scrub typhus vaccines: past history and recent developments. Hum Vaccin 3, 73 80. (Pubitemid 46717371)
3. Choi, M.S., Seong, S.Y., Kang, J.S., Kim, Y.W., Huh, M.S. Kim, I.S. (1999) Homotypic and heterotypic antibody responses to a 56-kilodalton proteins of Orientia tsutsugamushi. Infect Immun 11, 6194 6197.
4. Kim, I.S., Seong, S.Y., Woo, S.G., Choi, M.S. Chang, W.H. (1993) High-level expression of a 56-kilodalton protein gene (bor56) of Rickettsia tsutsugamushi Boryong and its application to enzyme-linked immunosorbent assays. ortRapid diagnosis of scrub typhus by a passive hemagglutination assay using recombinant 56-kilodalton polypeptide. J Clin Microbiol 31, 598 605.
5. Landegren, U. (1984) Measurement of cell numbers by means of the endogenous enzyme hexosaminidase. J Immunol Methods 67, 379 388.
6. Min, C.K., Yang, J.S., Kim, S., Choi, M.S., Kim, I.S. Cho, N.H. (2008) Genome-based construction of the metabolic pathways of Orientia tsutsugamushi and comparative analysis within the Rickettsiales order. Comp Funct Genomics 623145, 1 14. (Pubitemid 351917472)
7. Ni, Y.S., Chan, T.C., Chao, C.C., Richards, A.L., Dasch, G.A. Ching, W.M. (2005) Protection against scrub typhus by a plasmid vaccine encoding the 56-KD outer membrane protein antigen gene. Am J Trop Med Hyg 73, 936 941.
8. Ohashi, N., Nashimoto, H., Ikeda, H. Tamura, A. (1992) Diversity of immunodominant 56-kDa type-specific antigen (TSA) of Rickettsia tsutsugamushi. J Biol Chem 25, 12728 12735.
9. Pizarro-Cerdá, J. Cossart, P. (2006) Bacterial adhesion and entry into host cells. Cell 124, 715 727. (Pubitemid 43261447)
10. Ree, H.I., Kim, T.E., Lee, I.Y., Jeon, S.H., Hwang, U.W. Chang, W.H. (2001) Determination and geographical distribution of Orientia tsutsugamushi serotypes in Korea by nested polymerase chain reaction. Am J Trop Med Hyg 65, 528 534.
11. Sambrook, J., Fritxch, E. Maniatis, T. (1989) Molecular Cloning: A Laboratory Manual, 2nd edn ed. Nolan, C.
12. Seong, S.Y., Huh, M.S., Jang, W.J., Park, S.G., Kim, J.G., Woo, S.G., Choi, M.S., Kim, I.S. et al. (1997a) Induction of homologous immune response to Rickettsia tsutsugamushi Boryong with a partial 56-kilodalton recombinant antigen fused with the maltose-binding protein MBP-Bor56. Infect Immun 65, 1541 1545.
13. Seong, S.Y., Park, S.G., Huh, M.S., Jang, W.J., Kim, H.R., Han, T.H., Choi, M.S., Chang, W.H. et al. (1997b) Mapping of antigenic determinant regions of the Bor56 protein of Orientia tsutsugamushi. Infect Immun 65, 5250 5256.
14. Seong, S.Y., Kim, M.K., Lee, S.M., Odgerel, Z., Choi, M.S., Han, T.H., Kim, I.S., Kang, J.S. et al. (2001a) Neutralization epitopes on the antigenic domain II of the Orientia tsutsugamushi 56-kDa protein revealed by monoclonal antibodies. Vaccine 19, 2 9. (Pubitemid 30618948)
15. Seong, S.Y., Choi, M.S. Kim, I.S. (2001b) Orientia tsutsugamushi infection: overview and immune responses. Microbes Infect 3, 11 21. (Pubitemid 32172036)
16. Stover, C.K., Marana, D.P., Carter, J.M., Roe, B.A., Mardis, E. Oaks, E.V. (1990) The 56-kilodalton major protein antigen of Rickettsia tsutsugamushi:molecular cloning and sequence analysis of the sta56 gene and precise identification of a strain-specific epitope. Infect Immun 58, 2076 2084. (Pubitemid 20212278)
17. Tamura, A., Urakami, H. Tsuruhara, T. (1982) Purification of Rickettsia tsutsugamushi by percoll density gradient centrifugation. Microbiol Immunol 26, 321 328. (Pubitemid 12063625)
18. Tamura, A., Ohashi, N., Urakami, H., Takahashi, K. Oyanagi, M. (1985) Analysis of polypeptide composition and antigenic components of Rickettsia tsutsugamushi by polyacrylamide gel electrophoresis and immunoblotting. Infect Immun 48, 671 675. (Pubitemid 15021313)
19. Vezzulli, L., Pezzati, E., Repetto, B., Stauder, M., Giusto, G. Pruzzo, C. (2008) A general role for surface membrane proteins in attachment to chitin particles and copepods of environmental and clinical vibrios. Lett Appl Microbiol 46, 119 125.
20. Wang, S.S., Zhan, D.C., Peng, G.F., Pan, H., Xiao, H., Zeng, N.H., Huang, J.L. Wang, Z.B. (2003) Development of a recombinant protein-based enzyme-linked immunosorbent assay and its applications in field surveillance of rodent mice for presence of immunoglobulin G against Orientia tsutsugamushi. Clin Diagn Lab Immunol 10, 451 458.
21. Yu, Y., Wen, B., Wen, B., Niu, D., Chen, M. Qiu, L. (2005) Induction of protective immunity against scrub typhus with a 56-kilodalton recombinant antigen fused with a 47-kilodalton antigen of Orientia tsutsugamushi Karp. Am J Trop Med Hyg 72, 458 464. (Pubitemid 41278858)