Endophytic bacteria in long-term in vitro cultivated "axenic" pineapple microplants revealed by PCR-DGGE

World Journal of Microbiology and Biotechnology

Volumn 26, Issue 3, 2010, Pages 555-560

  Abreu Tarazi, Monita F ^a   Navarrete, Acácio A ^a   Andreote, Fernando D ^c   Almeida, Cristina V ^b   Tsai, Siu M ^a   Almeida, Marcílio ^a  

^a UNIVERSITY OF SÃO PAULO   (Brazil)

^b In vitro Palm Consultoria Ltda   (Brazil)

^c EMBRAPA CERRADOS   (Brazil)

Author keywords

Ananas comosus; Endophyte; Micropropagation; PCR DGGE

Indexed keywords

16S RRNA GENE; ACTINOBACTERIA; ANANAS COMOSUS; BACTERIAL COMMUNITY; BETAPROTEOBACTERIA; DENATURING GRADIENT GEL ELECTROPHORESIS; ENDOPHYTIC BACTERIA; IN-VITRO; MATURE LEAVES; MICROBIAL CONTAMINATION; MICROPLANTS; MICROPROPAGATION; MOLECULAR APPROACH; PCR-DGGE; PLANT CHLOROPLASTS; PLANT DEVELOPMENT; PLANT ORGANS;

AMPLIFICATION; BACTERIOLOGY; CHLOROPHYLL; DNA; ELECTROPHORESIS; GENES; NUCLEIC ACIDS; PLANTS (BOTANY); POLYMERASE CHAIN REACTION; RNA;

BACTERIA;

ACTINOBACTERIA; ALPHAPROTEOBACTERIA; ANANAS COMOSUS; BACTERIA (MICROORGANISMS); BETAPROTEOBACTERIA;

EID: 77950461845     PISSN: 09593993     EISSN: None     Source Type: Journal    
DOI: 10.1007/s11274-009-0191-3     Document Type: Article

References (21)

1. Almeida CV, Yara R, Almeida M (2005) Fungos endofíticos isolados de ápices caulinares de pupunheira cultivada in vivo e in vitro. Pesq agropec bras 40: 467-470. doi: 10. 1590/S0100-204X2005000500007.
2. Almeida CV, Andreote F, Yara R, Tanaka FAO, Azevedo JL, Almeida M (2009) Bacteriosomes in axenic plants: endophytes as stable endosymbionts. World. J Microbiol Biotechnol (in press).
3. Andreote FD, Lacava PT, Gai CS, Araujo WL, Maccheroni W, van Overbeek LS, van Elsas JD, Azevedo JL (2006) Model plants for studying the interaction between Methylobacterium mesophilicum and Xylella fastidiosa. Can J Microbiol 52: 419-426. doi: 10. 1139/W05-142.
4. Andreote FD, Rossetto PB, Mendes R, Avila LA, Labate CA, Pizzirani-Kleiner AA, Azevedo JL, Araújo WL (2009) Bacterial community in the rhizosphere and rhizoplane of wild type and transgenic eucalyptus. World J Microbiol Biotechnol. doi: 10. 1007/s11274-009-9990-9.
5. Araújo WL, Marcon J, Maccheroni W, van Elsas JD, van Vuurde JWL, Azevedo JL (2002) Diversity of endophytic bacterial populations and their interaction with Xylella fastidiosa in citrus plants. Appl Environ Microbiol 68: 4906-4914. doi: 10. 1128/AEM. 68. 10. 4906-4914. 2002.
6. Baudoin E, Benizri E, Guckert A (2003) Impact of artificial root exudates on the bacterial community structure in bulk soil and maize rhizosphere. Soil Biol Biochem 35: 1183-1192. doi:#10. 1016/S0038-0717(03)00179-2.
7. Bethlenfalvay GJ, Brown MS, Pacovsky RS (1982) Relationship between host and endophyte development in mycorrhizal soybean. New Phytol 90: 537-543. doi: 0028-646X/82/030537-I-07.
8. Chelius MK, Triplett EW (2001) The diversity of archaea and bacteria in association with the roots of Zea mays L. Microb Ecol 41: 252-263. doi: 10. 1007/s002480000087.
9. Dias ACF, Costa FEC, Andreote FD, Lacava PT, Teixeira MA, Assumpção LC, Araujo WL, Azevedo JL, Melo IS (2009) Isolation of micropropagated strawberry endophytic bacteria and assessment of their potential for plant growth promotion. World J Microbiol Biotechnol 25: 189-195. doi: 10. 1007/s11274-008-9878-0.
10. George EF, Hall MA, De Klerk GJ (2008) Plant propagation by tissue culture. Springer, Dordrecht.
11. Gomes NCM, Heuer H, Schonfeld J, Costa R, Mendonca-Hagler L, Smalla K (2001) Bacterial diversity of the rhizosphere of maize (Zea mays) grown in tropical soil studied by temperature gradient gel electrophoresis. Plant Soil 232: 167-180. doi: 10. 1023/A: 1010350406708.
12. Heuer H, Krsek M, Baker P, Smalla K, Wellington EMH (1997) Analysis of actinomycete communities by specific amplification of genes encoding 16S rRNA and gel-electrophoretic separation in denaturing gradients. Appl Environ Microbiol 63: 3233-3241. doi: 0099-2240/97.
13. Lodewyckx C, Vangronsveld J, Porteous F, Moore ERB, Taghavi S, Mesgeay M, van Der Lelie D (2002) Endophytic bacteria and their potential applications. Crit Rev Plant Sci 21: 583-606. doi: 10. 1080/0735-260291044377.
14. Murashige T, Skoog F (1962) A revised medium for rapid growth and bioassays with tobacco tissue cultures. Physiol Plant 15: 473-497. doi: 10. 1111/j. 1399-3054. 1962. tb08052. x.
15. Muyzer G, Dewaal EC, Uitterlinden AG (1993) Profiling of complex microbial populations by denaturing gradient gel electrophoresis analysis of polymerase chain reaction amplified genes coding for 16S ribosomal RNA. Appl Environ Microbiol 59: 695-700. doi: 0099-2240/93/030695-06.
16. Pirttilä AM, Laukkanen H, Pospiech H, Myllyla R, Hohtola A (2000) Detection of intracellular bacteria in the buds of Scotch fine (Pinus sylvestris L.) by in situ hybridization. Appl Environ Microbiol 66: 3073-3077. doi: 0099-2240/00.
17. Pospíšilová J, Čatský J, Solárová J, Tichá I (1987) Photosynthesis of plant regenerants. Specificity of in vitro conditions and plantlet response. Biol Plant 29: 415-421. doi: 10. 1007/BF02882209.
18. Roberts IM, Boevink P, Roberts AG, Sauer N, Reichel C, Oparka KJ (2001) Dynamic changes in the frequency and architecture of plasmodesmata during the sink-source transition in tobacco leaves. Protoplasma 218: 31-44. doi: 10. 1007/BF01288358.
19. Ryan RP, Germaine K, Franks A, Ryan DJ, Dowling DN (2008) Bacterial endophytes: recent developments and applications. FEMS Microbiol Lett 278: 1-9. doi: 10. 1111/j. 1574-6968. 2007. 00918. x.
20. Weber OB, Baldani VLD, Teixeira KRS, Kirchhof G, Baldani JI, Dobereiner J (1999) Isolation and characterization of diazotrophic bacteria from banana and pineapple plants. Plant Soil 210: 103-113. doi: 10. 1023/A: 1004623523179.
21. Weber OB, Lima RN, Crisóstomo LA, Freitas JAD, Carvalho ACPP, Maia AHN (2009) Effect of diazotrophic bacterium inoculation and organic fertilization on yield of Champaka pineapple intercropped with irrigated sapota. Plant Soil. doi: 10. 1007/s11104-009-0059-1.