Picomole-scale characterization of protein stability and function by quantitative cysteine reactivity

Proceedings of the National Academy of Sciences of the United States of America

Volumn 107, Issue 11, 2010, Pages 4908-4913

  Isom, Daniel G ^a   Vardy, Eyal ^a   Oas, Terrence G ^a   Hellinga, Homme W ^a  

^a DUKE UNIVERSITY MEDICAL CENTER   (United States)

Author keywords

Conformational stability; Ligand binding affinity; Linkage analysis; Thermal stability; Thiol protection

Indexed keywords

BINDING PROTEIN; MALTOSE BINDING PROTEIN; NUCLEASE; RIBOSE BINDING PROTEIN; UNCLASSIFIED DRUG;

ACCURACY; ARTICLE; BINDING AFFINITY; CONTROLLED STUDY; ESCHERICHIA COLI; FUNCTIONAL GENOMICS; LABORATORY APPARATUS, EQUIPMENT AND SUPPLIES; LIGAND BINDING; NONHUMAN; PRIORITY JOURNAL; PROTEIN ANALYSIS; PROTEIN CONFORMATION; PROTEIN ENGINEERING; PROTEIN FUNCTION; PROTEIN STABILITY; QUANTITATIVE ANALYSIS; QUANTITATIVE CYSTEINE REACTIVITY; STAPHYLOCOCCUS; TECHNIQUE;

CYSTEINE; ESCHERICHIA COLI PROTEINS; LIGANDS; MICROCOCCAL NUCLEASE; NANOTECHNOLOGY; PROTEIN CONFORMATION; PROTEIN FOLDING; PROTEIN STABILITY; PROTEINS; TEMPERATURE;

EID: 77950440622     PISSN: 00278424     EISSN: 10916490     Source Type: Journal    
DOI: 10.1073/pnas.0910421107     Document Type: Article

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