Mutational analysis of the 5′-OH oligonucleotide phosphate acceptor site of T4 polynucleotide kinase

Nucleic Acids Research

Volumn 38, Issue 4, 2009, Pages 1304-1311

  Wang, Li Kai ^a   Shuman, Stewart ^a  

^a MEMORIAL SLOAN KETTERING CANCER CENTER   (United States)

Author keywords

[No Author keywords available]

Indexed keywords

ARGININE; ASPARTIC ACID; GLUTAMIC ACID; GLYCINE; OLIGONUCLEOTIDE; PHENYLALANINE; POLYNUCLEOTIDE 5' HYDROXYL KINASE; PROLINE; VALINE; PHOSPHATE; VIRUS PROTEIN;

AMINO ACID SUBSTITUTION; ARTICLE; BACTERIOPHAGE T4; BINDING SITE; CATALYSIS; CONTROLLED STUDY; ENZYME ACTIVITY; MUTAGENESIS; MUTATIONAL ANALYSIS; NONHUMAN; PRIORITY JOURNAL; BIOCATALYSIS; CHEMICAL STRUCTURE; CHEMISTRY; ENZYMOLOGY; GENETICS; METABOLISM; MUTATION;

BACTERIOPHAGE T4; BIOCATALYSIS; GLUTAMIC ACID; MODELS, MOLECULAR; MUTAGENESIS; MUTATION; OLIGONUCLEOTIDES; PHOSPHATES; POLYNUCLEOTIDE 5'-HYDROXYL-KINASE; VIRAL PROTEINS;

EID: 77950360500     PISSN: 03051048     EISSN: 13624962     Source Type: Journal    
DOI: 10.1093/nar/gkp1096     Document Type: Article

References (18)

1. Amitsur, M., Levitz, R. and Kaufman, G. (1987) Bacteriophage T4 anticodon nuclease, polynucleotide kinase, and RNA ligase reprocess the host lysine tRNA. EMBO J., 6, 2499-2503.
2. Richardson, C.C. (1965) Phosphorylation of nucleic acid by an enzyme from T4 bacteriophage-infected Escherichia coli. Proc. Natl Acad. Sci. USA, 54, 158-165.
3. Novogrodsky, A. and Hurwitz, J. (1966) The enzymatic phosphorylation of ribonucleic acid and deoxyribonucleic acid: phosphorylation at 5′-hydroxyl termini. J. Biol. Chem., 241, 2923-2932.
4. Novogrodsky, A., Tal, M., Traub, A. and Hurwitz, J. (1966) The enzymatic phosphorylation of ribonucleic acid and deoxyribonucleic acid: further properties of the 5′-hydroxyl polynucleotide kinase. J. Biol. Chem., 241, 2933-2943.
5. Becker, A. and Hurwitz, J. (1967) The enzymatic cleavage of phosphate termini from polynucleotides. J. Biol. Chem., 242, 936-950.
6. Cameron, V. and Uhlenbeck, O.C. (1977) 3′-Phosphatase activity in T4 polynucleotide kinase. Biochemistry, 16, 5120-5126.
7. Wang, L.K., Lima, C.D. and Shuman, S. (2002) Structure and mechanism of T4 polynucleotide kinase - an RNA repair enzyme. EMBO J., 21, 3873-3880.
8. Galburt, E.A., Pelletier, J., Wilson, G. and Stoddard, B.L. (2002) Structure of a tRNA repair enzyme and molecular biology workhorse: T4 polynucleotide kinase. Structure, 10, 1249-1260.
9. Eastberg, J.H., Pelletier, J. and Stoddard, B.L. (2004) Recognition of DNA substrates by bacteriophage T4 polynucleotide kinase. Nucleic Acids Res., 32, 653-660.
10. Zhu, H., Smith, P., Wang, L.K. and Shuman, S. (2007) Structure-function analysis of the 3′-phosphatase component of T4 polynucleotide kinase/phosphatase. Virology, 366, 126-136.
11. Wang, L.K. and Shuman, S. (2001) Domain structure and mutational analysis of T4 polynucleotide kinase. J. Biol. Chem., 276, 26868-26874.
12. Wang, L.K. and Shuman, S. (2002) Mutational analysis defines the 5′-kinase and 3′-phosphatase active sites of T4 polynucleotide kinase. Nucleic Acids Res., 30, 1073-1080.
13. Karimi-Busheri, F. and Weinfeld, M. (1997) Purification and substrate specificity of polydeoxynucleotide kinases isolated from calf thymus and rat liver. J. Cell. Biochem., 64, 258-272.
14. Zhu, H., Yin, S. and Shuman, S. (2004) Characterization of polynucleotide kinase/phosphatase enzymes from mycobacteriophages Omega and Cjw1 and vibriophage KVP40. J. Biol. Chem., 279, 26358-26369.
15. Schwer, B., Sawaya, R., Ho, C.K. and Shuman, S. (2004) Portability and fidelity of RNA-repair systems. Proc. Natl Acad. Sci. USA, 101, 2788-2793.
16. Bernstein, N.K., Williams, R.S., Rakovszky, M.L., Cui, D., Green, R., Galicia, S., Koch, C.A., Cass, C.E., Durocher, D., Weinfeld, M. et al. (2005) The molecular architecture of the mammalian DNA repair enzyme, polynucleotide kinase. Mol. Cell, 17, 657-670.
17. Bernstein, N.K., Hammel, M., Mani, R.S., Weinfeld, M., Pelikan, M., Tainer, J.A. and Glover, J.N.M. (2005) Mechanism of DNA substrate recognition by the mammalian DNA repair enzyme, polynucleotide kinase. Nucleic Acids Res., 37, 6161-6173.
18. Jilani, A., Slack, C., Matheos, D., Zannis-Hadjopoulos, M. and Lasko, D.D. (1999) Purification of a polynucleotide kinase from calf thymus, comparison of its 3′-phosphatase domain with T4 polynucleotide kinase, and investigation of its effect on DNA replication in vitro. J. Cell. Biochem., 73, 188-203.