Activity of immobilised rat hepatic microsomal CYP2E1 using alumina membrane as a support

New Biotechnology

Volumn 26, Issue 5, 2009, Pages 222-228

  Tanvir, Shazia ^a   Morandat, Sandrine ^a   Frederic, Nadaud ^a   Adenier, Hervé ^a   Pulvin, Sylviane ^a  

^a UNIVERSITÉ DE TECHNOLOGIE DE COMPIÈGNE   (France)

Author keywords

[No Author keywords available]

Indexed keywords

ALUMINA MEMBRANES; AMINE GROUPS; BIO-CATALYTIC REACTORS; HYDROXYLASE ACTIVITY; IMMOBILISED ENZYMES; MICROSOMAL MEMBRANES; PARA-NITROPHENOL; POROUS ALUMINA MEMBRANES; RAT LIVERS; RESIDENCE TIME; TISSUE SOURCES;

ACTIVATED ALUMINA; BIOREACTORS; BIOSENSORS; FILM GROWTH; RATS; TISSUE;

MEMBRANES;

4 NITROCATECHOL; 4 NITROPHENOL; 4-NITROCATECHOL; 4-NITROPHENOL; ALUMINUM OXIDE; CATECHOL DERIVATIVE; CYTOCHROME P450 2E1; GOLD; IMMOBILIZED ENZYME; NITROPHENOL;

ADSORPTION; ANIMAL; ARTICLE; ARTIFICIAL MEMBRANE; BIOREACTOR; CHEMISTRY; ENZYMOLOGY; HYDROXYLATION; LIVER MICROSOME; MALE; METABOLISM; RAT; SPRAGUE DAWLEY RAT; TIME; ULTRASTRUCTURE;

ADSORPTION; ALUMINUM OXIDE; ANIMALS; BIOREACTORS; CATECHOLS; CYTOCHROME P-450 CYP2E1; ENZYMES, IMMOBILIZED; GOLD; HYDROXYLATION; MALE; MEMBRANES, ARTIFICIAL; MICROSOMES, LIVER; NITROPHENOLS; RATS; RATS, SPRAGUE-DAWLEY; TIME FACTORS;

EID: 77950349404     PISSN: 18716784     EISSN: 18764347     Source Type: Journal    
DOI: 10.1016/j.nbt.2009.08.004     Document Type: Article

References (33)

1. Gekas V.C. Artificial membranes as carriers for the immobilization of biocatalysts. Enzyme Microb. Technol. 1986, 8:450-460.
2. Tanvir S., et al. Development of immobilization technique for liver microsomes. Colloid. Surf. B Biointerface 2009, 68:178-183.
3. Milka P., et al. Immobilization of alliinase on porous aluminum oxide. Biotechnol. Bioeng. 2000, 69:344-348.
4. Edwards R.J., et al. Orientation of cytochromes P450 in the endoplasmic reticulum. Biochemistry 2002, 30:71-76.
5. Lieber C.S. Cytochrome P-4502E1: its physiological and pathological role. Physiol. Rev. 1997, 77:517-544.
6. Vieira I., et al. Developmental expression of CYP2E1 in the human liver hypermethylation control of gene expression during the neonatal period. Eur. J. Biochem. 1996, 238:476-483.
7. Gonzalez-Jasso E., et al. CYP2E1 regulation by benzene and other small organic chemicals in rat liver and peripheral lymphocytes. Toxicol. Lett. 2003, 144:55-67.
8. Tanaka E., et al. Cytochrome P450 2E1: its clinical and toxicological role. J. Clin. Pharm. Ther. 2000, 25:165-175.
9. Czekaj P., et al. Tobacco smoke-dependent changes in cytochrome P450 1A1, 1A2, and 2E1 protein expressions in fetuses, newborns, pregnant rats, and human placenta. Arch. Toxicol. 2005, 79:13-24.
10. Court M.H., et al. Biotransformation of chlorzoxazone by hepatic microsomes from humans and ten other mammalian species. Biopharm. Drug Dispos. 1997, 18:213-226.
11. Casazza J.P., et al. The metabolism of acetone in rat. J. Biol. Chem. 1984, 259:231-236.
12. Lin H., Hollenberg P.F. N-nitrosodimethylamine-mediated formation of oxidized and methylated DNA bases in a cytochrome P450 2E1 expressing cell line. Chem. Res. Toxicol. 2001, 14:562-566.
13. Pearce R.E., et al. Effects of freezing, thawing and storing human liver microsomes on cytochrome P450 activity. Biochem. Biophys. 1996, 331:145169.
14. Kobayashi K., et al. Substrate specificity for rat cytochrome P450 (CYP) isoforms: screening with cDNA-expressed systems of the rat. Biochem. Pharmacol. 2002, 63:889-896.
15. Tassaneeyakul W., et al. High-performance liquid chromatographic assay for 4-nitrophenol hydroxylation, a putative cytochrome P-4502E1 activity, in human liver microsomes. J. Chromatogr. 1993, 616:73-78.
16. Amato G., et al. Chlorzoxazone 6-hydroxylase and p-nitrophenol hydroxylase as the most suitable activities for assaying cytochrome P450 2E1 in Cynomolgus monkey liver. Drug Metab. Dispos. 1998, 26:483-489.
17. 14C]p-nitrophenol in rats. Arch. Toxicol. 2000, 74:388-396.
18. Duescher R.J., Elfarra A.A. Determination of p-nitrophenol hydroxylase activity of rat liver microsomes by high-pressure liquid chromatography. Anal. Biochem. 1993, 212:311-314.
19. Mi L.J., et al. Attenuation of alcohol-induced apoptosis of hepatocytes in rat livers by polyenylphosphatidylcholine (PPC). Alcohol Clin. Exp. Res. 2000, 24:207-212.
20. Puccini P., et al. Purification and characterization of an acetone-inducible cytochrome P-450 from hamster liver microsomes. Biochem. J. 1992, 287:863-870.
21. Lowry O.H., et al. Protein measurement with the Folin phenol reagent. J. Biol. Chem. 1951, 193:265-275.
22. Omura T., Sato R. The carbon monoxide binding pigment of liver microsomes. J. Biol. Chem. 1964, 239:2370-2385.
23. Mathews J.M., et al. Selective inhibition of cytochrome P450 2E1 in vivo and in vitro with trans-1,2-dichloroethylene. Chem. Res. Toxicol. 1998, 11:778-785.
24. Tassaneeyakul W., et al. Validation of 4-nitrophenol as an in vitro substrate probe for human liver CYP2E1 using cDNA expression and microsomal kinetic techniques. Biochem. Pharmacol. 1993, 46:1975-1981.
25. Draper A., et al. Role of CYP2E1 and CYP2A6 in the hydroxylation of 4-nitrophenol by human liver microsomes. Toxicologist 1996, 30:319-323.
26. Tesoro G., Yulong W. Silane coupling agents: the role of the organofunctional group. J. Adhes. Sci. Technol. 1991, 5:771-784.
27. Weetall H.H. Preparation of immobilized protein coupled through silane coupling agents to inorganic supports. Appl. Biochem. Biotechnol. 1993, 41:157-188.
28. Reinke L.A., Moyer M.J. pNitrophenol hydroxylation: a microsomal oxidation which is highly inducible by ethanol. Drug Metab. Dispos. 1985, 13:548-552.
29. Vorsilak P., et al. Enzyme immobilized in a membrane sandwich reactor. J. Food Sci. 2006, 40:431-432.
30. Li N., Sakaki K. Performance of an emulsion enzyme membrane reactor combined with premix membrane emulsification for lipase-catalyzed resolution of enantiomers. J. Membr. Sci. 2008, 314:183-192.
31. Carpenter J.F., Crowe J.H. An infrared spectroscopic study of the interactions of carbohydrates with dried proteins. Biochemistry 1989, 28:3916-3922.
32. Crowe J.H., et al. Stabilization of dry phospholipid bilayers and proteins by sugars. Biochem. J. 1987, 242:1-10.
33. Chiantia S., et al. Dehydration damage of domain-exhibiting supported bilayers: an AFM study on the protective effects of disaccharides and other stabilizing substances. Langmuir 2005, 21:6317-6323.